Mutation in HvCBP20 (Cap Binding Protein 20) Adapts Barley to Drought Stress at Phenotypic and Transcriptomic Levels

Daszkowska‐Golec, Agata; Skubacz, Anna; Marzec, Marek; Slota, Michal; Kurowska, Marzena; Gajecka, Monika; Gajewska, Patrycja; Płociniczak, Tomasz; Sitko, Krzysztof; Pacak, Andrzej; Szweykowska-Kulińska, Zofia; Szarejko, Iwona

doi:10.3389/fpls.2017.00942

Cited by 25 publications

(21 citation statements)

References 112 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The Hor TILLUS platform has already proven its usefulness in functional genomics studies. The platform has been utilized to reveal function of genes involved in brassinosteroid and strigolactone metabolism (Gruszka et al, 2016 ; Marzec et al, 2016 ), DNA repair (Stolarek et al, 2015a , b ), tolerance to abiotic stresses (Mendiondo et al, 2016 ; Daszkowska-Golec et al, 2017 ). In addition, the individual collection of ca.…”

Section: Discussionmentioning

confidence: 99%

“…It can serve as a platform for functional genomics and pre-breeding programs of barley. The Hor TILLUS population has proved its utility as a reverse genetics tool in many barley studies concerning e.g., brassinosteroid metabolism (Gruszka et al, 2016 ), DNA repair (Stolarek et al, 2015a , b ), strigolactone signaling (Marzec et al, 2016 ), waterlogging tolerance (Mendiondo et al, 2016 ), or drought and ABA response (Daszkowska-Golec et al, 2017 ). This population is being constantly renewed to make it functional for many years.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

HorTILLUS—A Rich and Renewable Source of Induced Mutations for Forward/Reverse Genetics and Pre-breeding Programs in Barley (Hordeum vulgare L.)

et al. 2018

Self Cite

View full text Add to dashboard Cite

TILLING (Targeting Induced Local Lesions IN Genomes) is a strategy used for functional analysis of genes that combines the classical mutagenesis and a rapid, high-throughput identification of mutations within a gene of interest. TILLING has been initially developed as a discovery platform for functional genomics, but soon it has become a valuable tool in development of desired alleles for crop breeding, alternative to transgenic approach. Here we present the HorTILLUS (Hordeum—TILLING—University of Silesia) population created for spring barley cultivar “Sebastian” after double-treatment of seeds with two chemical mutagens: sodium azide (NaN3) and N-methyl-N-nitrosourea (MNU). The population comprises more than 9,600 M2 plants from which DNA was isolated, seeds harvested, vacuum-packed, and deposited in seed bank. M3 progeny of 3,481 M2 individuals was grown in the field and phenotyped. The screening for mutations was performed for 32 genes related to different aspects of plant growth and development. For each gene fragment, 3,072–6,912 M2 plants were used for mutation identification using LI-COR sequencer. In total, 382 mutations were found in 182.2 Mb screened. The average mutation density in the HorTILLUS, estimated as 1 mutation per 477 kb, is among the highest mutation densities reported for barley. The majority of mutations were G/C to A/T transitions, however about 8% transversions were also detected. Sixty-one percent of mutations found in coding regions were missense, 37.5% silent and 1.1% nonsense. In each gene, the missense mutations with a potential effect on protein function were identified. The HorTILLUS platform is the largest of the TILLING populations reported for barley and best characterized. The population proved to be a useful tool, both in functional genomic studies and in forward selection of barley mutants with required phenotypic changes. We are constantly renewing the HorTILLUS population, which makes it a permanent source of new mutations. We offer the usage of this valuable resource to the interested barley researchers on cooperative basis.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

HorTILLUS—A Rich and Renewable Source of Induced Mutations for Forward/Reverse Genetics and Pre-breeding Programs in Barley (Hordeum vulgare L.)

et al. 2018

Self Cite

View full text Add to dashboard Cite

show abstract

“…The authors pointed out the importance of the crosstalk pathways of these and other phytohormones at different molecular targets with synergic or antagonist effects. Thus, the expression of the gene NCED, involved directly in the synthesis of ABA, had an important effect in the response to abiotic stress but also in the seed germination and maturation, highlighting the crosslink between both events [132]. Alterations in the transporters that allow for ABA storage and translocation were also observed under drought [133] or salt and osmotic stress [134,135].…”

Section: Seed Genes To Cope With Abiotic Stressmentioning

confidence: 96%

The Importance of Ion Homeostasis and Nutrient Status in Seed Development and Germination

et al. 2020

View full text Add to dashboard Cite

Seed is the dissemination unit of plants initiating an important stage in the life cycle of plants. Seed development, comprising two phases: embryogenesis and seed maturation, may define the quality of sown seed, especially under abiotic stress. In this review we have focused on the recent advances in the molecular mechanisms underlying these complex processes and how they are controlled by distinct environmental factors regulating ion homeostasis into the seed tissues. The role of transporters affecting seed embryogenesis and first stages of germination as imbibition and subsequent radicle protrusion and extension were revised from a molecular point of view. Seed formation depends on the loading of nutrients from the maternal seed coat to the filial endosperm, a process of which the efflux is not clear and where different ions and transporters are involved. The clear interrelation between soil nutrients, presence of heavy metals and the ion capacity of penetration through the seed are discussed in terms of ion effect during different germination stages. Results concerning seed priming techniques used in the improvement of seed vigor and radicle emergence are shown, where the use of nutrients as a novel way of osmopriming to alleviate abiotic stress effects and improve seedlings yield is discussed. Novel approaches to know the re-translocation from source leaves to developing seeds are considered, as an essential mechanism to understand the biofortification process of certain grains in order to cope with nutrient deficiencies, especially in arid and semiarid areas. Finally, the role of new genes involved in hormone-dependent processes, oxidative response and water uptake into the seeds during their development or germination, have been described as plant mechanisms to deal with abiotic stresses.

show abstract

“…"Cam/B1" and "Maresi" are the parents of the mapping population used in our previous studies on mapping the Quantitative Trait Loci (QTLs) that are related to drought-stress tolerance in barley [24] and the cultivar "Sebastian" is the parent of the Targeting Induced Local Lesions in Genomes (TILLING) population (HorTILLUS) developed after chemical mutagenesis at the University of Silesia in Katowice [60]. This population has been used to develop mutants in the genes of interest, including the genes related to abiotic stresses, including drought [22,23].…”

Section: Plant Materialsmentioning

confidence: 99%

“…Drought stress was applied, as described earlier [23]. Briefly, the experiment was carried out in boxes (400 × 140 × 175 mm) filled with soil containing a mixture of sandy loam and sand (7:2) with known physicochemical properties.…”

Section: Drought Stress Experimentsmentioning

confidence: 99%

Genetic and Physiological Dissection of Photosynthesis in Barley Exposed to Drought Stress

Daszkowska‐Golec

Collin

Sitko

et al. 2019

IJMS

Self Cite

View full text Add to dashboard Cite

Balanced photosynthesis under drought is essential for better survival and for agricultural benefits in terms of biomass and yield. Given the current attempts to improve the photosynthetic efficiency for greater crop yield, the explanation of the genetic basis of that process, together with the phenotypic analysis, is significant in terms of both basic studies and potential agricultural application. Therefore, the main objective of this study was to uncover the molecular basis of the photosynthesis process under drought stress in barley. To address that goal, we conducted transcriptomic examination together with detailed photosynthesis analysis using the JIP-test. Using this approach, we indicated that photosynthesis is a process that is very early affected in barley seedlings treated with severe drought stress. Rather than focusing on individual genes, our strategy was pointed to the identification of groups of genes with similar expression patterns. As such, we identified and annotated almost 150 barley genes as crucial core-components of photosystems, electron transport components, and Calvin cycle enzymes. Moreover, we designated 17 possible regulatory interactions between photosynthesis-related genes and transcription factors in barley. Summarizing, our results provide a list of candidate genes for future genetic research and improvement of barley drought tolerance by targeting photosynthesis.

show abstract

Mutation in HvCBP20 (Cap Binding Protein 20) Adapts Barley to Drought Stress at Phenotypic and Transcriptomic Levels

Cited by 25 publications

References 112 publications

HorTILLUS—A Rich and Renewable Source of Induced Mutations for Forward/Reverse Genetics and Pre-breeding Programs in Barley (Hordeum vulgare L.)

HorTILLUS—A Rich and Renewable Source of Induced Mutations for Forward/Reverse Genetics and Pre-breeding Programs in Barley (Hordeum vulgare L.)

The Importance of Ion Homeostasis and Nutrient Status in Seed Development and Germination

Genetic and Physiological Dissection of Photosynthesis in Barley Exposed to Drought Stress

Contact Info

Product

Resources

About