Overexpression of the maize E3 ubiquitin ligase gene ZmAIRP4 enhances drought stress tolerance in Arabidopsis

Yang, Liang; Wu, Lintao; Chang, Wei; Li, Zhi; Miao, Mingjun; YueJian, Li; Yang, Junpin; Liu, Zhibin; Tan, Jun

doi:10.1016/j.plaphy.2017.11.017

Cited by 39 publications

(25 citation statements)

References 35 publications

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“…Others have also reported that the E3 ubiquitin ligase gene ZmAIRP4 plays an active role in enhancing drought stress tolerance (Yang et al, 2018). Further, overexpression of TaSAP5 increases the drought tolerance of Arabidopsis and wheat seedlings .…”

Section: Discussionmentioning

confidence: 95%

“…On the one hand, it has been reported that RZFP34/CHYR1, a RING‐type ubiquitin E3 ligase of Arabidopsis , enhances drought resistance (Ding, Zhang, & Qin, ). Others have also reported that the E3 ubiquitin ligase gene ZmAIRP4 plays an active role in enhancing drought stress tolerance (Yang et al., ). Further, overexpression of TaSAP5 increases the drought tolerance of Arabidopsis and wheat seedlings (Zhang, Yin, et al., ).…”

Section: Discussionmentioning

confidence: 99%

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Identification of plant hormones and candidate hub genes regulating flag leaf senescence in wheat response to water deficit stress at the grain‐filling stage

et al. 2019

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In order to clarify the transcriptional regulatory network and physiological mechanisms governing leaf senescence response to drought stress in wheat, experiments were performed using two wheat varieties with contrasting drought tolerance: Fu287 (F287, a drought‐sensitive genotype) and Shannong20 (SN20, a drought‐resistant genotype). The latter has higher SPAD values, salicylic acid (SA), jasmonic acid (JA), zeatin (Z), zeatin riboside (ZR), and gibberellin (GA3) content as well as higher expression levels of Cu/Zn‐SOD, Mn‐SOD, Fe‐SOD, POD, CAT, and APX under various water deficit conditions. Conjoint analysis of physiological and biochemical indicators and transcriptome data by weighted gene co‐expression network analysis (WGCNA) in the present study provides a useful genomic and molecular resource for studying drought adaptation in wheat. The flag leaf senescence process was changed by altering the concentration of phytohormones. SA, JA, abscisic acid (ABA), Z, ZR, and GA3 coordinate with each other to control leaf senescence and plant adaptation under drought stress. Further, the leaf senescence process was divided into two phases: the persistence phase and the rapid loss phase. Shorter Chltotal (duration of the flag leaf being photosynthetically active), shorter Chlper (persistence phase), reduced M (inflection point cumulative temperature when senescence rate is the maximum), decreased rmax (the maximum senescence rate), larger r0 (the initial senescence rate), and increased raver (the average senescence rate) were slightly associated with low grain mass. We speculated that extending the period of the persistence phase by cultivation or chemical control measures could further increase the drought survivability and productivity of wheat.

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Section: Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 99%

Identification of plant hormones and candidate hub genes regulating flag leaf senescence in wheat response to water deficit stress at the grain‐filling stage

et al. 2019

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“…The first is the protein KZV45975.1, member of the ortholog group "plantannot11681", which had members related to "E3 ubiquitin ligase family of proteins". This family of proteins seems to enhance drought tolerance in Arabidopsis thaliana [38]. Another interesting example is the KZV43328.1 protein, member of "plantannot19415" ortholog group, which have 5 members with the PFAM domain "PF00642 -Zinc finger C-x8-C-x5-C-x3-H type (and similar) (zf-CCCH)".…”

Section: Boea Hygrometrica (Dorcoceras Hygrometricum)mentioning

confidence: 99%

Plant Co-expression Annotation Resource: a webserver for identifying targets for genetically modified crop breeding pipelines

Viana¹,

Zerlotini

Mudadu

2020

Preprint

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BackgroundThe development of genetically modified crops (GM) includes the discovery of candidate genes through bioinformatics analysis using genomics data, gene expression, and others. Proteins of unknown function (PUFs) are interesting targets for GM crops breeding pipelines for the novelty associated to such targets and also to avoid copyright protections. One method of inferring the putative function of PUFs is by relating them to factors of interest such as abiotic stresses using orthology and co-expression networks, in a guilt-by-association manner. ResultsIn this regard, we have downloaded, analyzed, and processed genomics data of 53 angiosperms, totaling 1,862,010 genes and 2,332,974 RNA. Diamond and InterproScan were used to discover 72,266 PUFs for all organisms. RNA-seq datasets related to abiotic stresses were downloaded from NCBI/GEO. The RNA-seq data was used as input to the LSTrAP software to construct co-expression networks. LSTrAP also created clusters of transcripts with correlated expression, whose members are more probably related to the molecular mechanisms associated to abiotic stresses in the plants.Orthologous groups were created (OrhtoMCL) using all 2,332,974 proteins in order to associate PUFs to abiotic stress related clusters of co-expression and therefore infer their function in a guilt-byassociation manner. ConclusionA freely available web resource named "Plant Co-expression Annotation Resource" (https://www.machado.cnptia.embrapa.br/plantannot), Plantannot, was created to provide indexed queries to search for PUF putatively associated to abiotic stresses. The web interface also allows browsing, querying and retrieving of public genomics data from 53 plants. We hope Plantannot to be useful for researchers trying to obtain novel GM crops resistant to climate change hazards. BACKGROUNDIn the last decades, the ability to genetically engineer plants with success showed the potential to create genetically modified (GM) crops with favourable economic outcomes [1]. As well, in the last decades, the main achievements in this area were genetically improved plants tolerant to herbicide and resistant to insects. Others, like nutritional composition improvements are ongoing [2]. Furthermore, new mechanisms for genome editing are improving the accuracy and speed of genome modifications in plants, such as the CRISPR/CAS system [3,4].Regarding climate change and environmental factors, plants are being genetically modified to become resilient to abiotic stresses, such as drought, high temperature, rising atmospheric CO2, to potentially overcome the yield losses due to these factors [5,6].Intellectual property rights (IPR) are vastly used by biotechnology enterprises for their GM plants, to allow exclusive rights and provide better returns for the high investments in research and development [7]. In this way, over the last years many patents applications for genetically improved crops regarding stress tolerance were filled [8].

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“…This suggests that ABA sensitivity is regulated by the expression of different genes involved in ABA perception/signaling. In studies using transgenic lines, overexpression of genes involved in ABA signaling such as the aspartic protease ASPG1, the NADP-malic enzyme or an E3 ubiquitin ligase enhanced tolerance to drought stress [25][26][27]. In another study, the loss of function through antisense regulation or by mutation of the receptor-like kinase1 (RPK1) in Arabidopsis decreased ABA sensitivity, stomatal closure and the expression of several stress-inducible genes such as LEAlike proteins, peroxidase, RD26, DnaJ-like protein, cytochrome P450 and SOD [28].…”

Section: Introductionmentioning

confidence: 99%

The barley stripe mosaic virus expression system reveals the wheat C2H2 zinc finger protein TaZFP1B as a key regulator of drought tolerance

2020

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Background: Drought stress is one of the major factors limiting wheat production globally. Improving drought tolerance is important for agriculture sustainability. Although various morphological, physiological and biochemical responses associated with drought tolerance have been documented, the molecular mechanisms and regulatory genes that are needed to improve drought tolerance in crops require further investigation. We have used a novel 4-component version (for overexpression) and a 3-component version (for underexpression) of a barley stripe mosaic virus-based (BSMV) system for functional characterization of the C2H2-type zinc finger protein TaZFP1B in wheat. These expression systems avoid the need to produce transgenic plant lines and greatly speed up functional gene characterization. Results: We show that overexpression of TaZFP1B stimulates plant growth and up-regulates different oxidative stressresponsive genes under well-watered conditions. Plants that overexpress TaZFP1B are more drought tolerant at critical periods of the plant's life cycle. Furthermore, RNA-Seq analysis revealed that plants overexpressing TaZFP1B reprogram their transcriptome, resulting in physiological and physical modifications that help wheat to grow and survive under drought stress. In contrast, plants transformed to underexpress TaZFP1B are significantly less tolerant to drought and growth is negatively affected. Conclusions: This study clearly shows that the two versions of the BSMV system can be used for fast and efficient functional characterization of genes in crops. The extent of transcriptome reprogramming in plants that overexpress TaZFP1B indicates that the encoded transcription factor is a key regulator of drought tolerance in wheat.

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Overexpression of the maize E3 ubiquitin ligase gene ZmAIRP4 enhances drought stress tolerance in Arabidopsis

Cited by 39 publications

References 35 publications

Identification of plant hormones and candidate hub genes regulating flag leaf senescence in wheat response to water deficit stress at the grain‐filling stage

Identification of plant hormones and candidate hub genes regulating flag leaf senescence in wheat response to water deficit stress at the grain‐filling stage

Plant Co-expression Annotation Resource: a webserver for identifying targets for genetically modified crop breeding pipelines

The barley stripe mosaic virus expression system reveals the wheat C2H2 zinc finger protein TaZFP1B as a key regulator of drought tolerance

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