Overexpression of thaumatin-like protein in transgenic tomato plants confers enhanced resistance to<i>Alternaria solani</i>

Radhajeyalakshmi, R.; Velazhahan, R.; Balasubramanian, P.; Doraiswamy, Sabitha

doi:10.1080/03235400400027382

Cited by 13 publications

(3 citation statements)

References 33 publications

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“…Jayaraj et al 43 demonstrated that the purified 23 kDa rice TLP (TLP‐D34), expressed and purified through an Escherichia coli expression system, inhibited the mycelial growth of many fungi, including Fusarium graminearum and Curvularia lunata , the fungi associated with grain mold of sorghum. Furthermore, it has been demonstrated that transgenic crop plants overexpressing TLP have enhanced resistance against various pathogens 44–47. Vigers et al 33 reported the presence of a 22 kDa TLP (sormatin) in sorghum seeds cross‐reacting with zeamatin antiserum.…”

Section: Discussionmentioning

confidence: 99%

Effect of formulated zimmu (Allium cepa L. × Allium sativum L.) extract in the management of grain mold of sorghum

et al. 2007

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Section: Discussionmentioning

confidence: 99%

Effect of formulated zimmu (Allium cepa L. × Allium sativum L.) extract in the management of grain mold of sorghum

et al. 2007

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“…Plant TLP mainly inhibits pathogenic and non-pathogenic fungi by lysing fungal spores, inhibiting spore germination, and reducing the vitality of young mycelium [ 4 , 5 ]. Recently, TLP genes were found to confer resistance to pathogenic fungi, including sclerotia, powdery mildew, and Pseudomonas syringae [ 6 , 7 ]. TLP transgenic plants can delay the development of a variety of fungal diseases and enhance plant resistance to pathogenic fungi [ 8 , 9 ].…”

Section: Introductionmentioning

confidence: 99%

Tomato-Thaumatin-like Protein Genes Solyc08g080660 and Solyc08g080670 Confer Resistance to Five Soil-Borne Diseases by Enhancing β-1,3-Glucanase Activity

et al. 2023

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Although thaumatin-like proteins (TLPs) are involved in resistance to a variety of fungal diseases, whether the TLP5 and TLP6 genes in tomato plants (Solanum lycopersicum) confer resistance to the pathogenesis of soil-borne diseases has not been demonstrated. In this study, five soil-borne diseases (fungal pathogens: Fusarium solani, Fusarium oxysporum, and Verticillium dahliae; bacterial pathogens: Clavibacter michiganense subsp. michiganense and Ralstonia solanacearum) were used to infect susceptible “No. 5” and disease-resistant “S-55” tomato cultivars. We found that SlTLP5 and SlTLP6 transcript levels were higher in susceptible cultivars treated with the three fungal pathogens than in those treated with the two bacterial pathogens and that transcript levels varied depending on the pathogen. Moreover, the SlTLP5 and SlTLP6 transcript levels were much higher in disease-resistant cultivars than in disease-susceptible cultivars, and the SlTLP5 and SlTLP6 transcript levels were higher in cultivars treated with the same fungal pathogen than in those treated with bacterial pathogens. SlTLP6 transcript levels were higher than SlTLP5. SlTLP5 and SlTLP6 overexpression and gene-edited transgenic mutants were generated in both susceptible and resistant cultivars. Overexpression and knockout increased and decreased resistance to the five diseases, respectively. Transgenic plants overexpressing SlTLP5 and SlTLP6 inhibited the activities of peroxidase (POD), superoxide dismutase (SOD), ascorbate peroxidase (APX), and catalase (CAT) after inoculation with fungal pathogens, and the activities of POD, SOD, and APX were similar to those of fungi after infection with bacterial pathogens. The activities of CAT were increased, and the activity of β-1,3-glucanase was increased in both the fungal and bacterial treatments. Overexpressed plants were more resistant than the control plants. After SlTLP5 and SlTLP6 knockout plants were inoculated, POD, SOD, and APX had no significant changes, but CAT activity increased and decreased significantly after the fungal and bacterial treatments, contrary to overexpression. The activity of β-1,3-glucanase decreased in the treatment of the five pathogens, and the knocked-out plants were more susceptible to disease than the control. In summary, this study contributes to the further understanding of TLP disease resistance mechanisms in tomato plants.

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“…tomato (Lincoln et al ., ). Also, overexpression of a thaumatin‐like protein in transgenic tomato plants has been shown to confer enhanced resistance to A. solani (Radhajeyalakshmi et al ., ) and in a study in which genes coding for a maize β‐glucanase and a Mirabilis jalapa antimicrobial peptide were introduced into tomato, enhanced resistance to early blight disease was observed (Schaefer et al ., ).…”

Section: Introductionmentioning

confidence: 99%

Tomato plants transformed with the inhibitor‐of‐virus‐replication gene are partially resistant to several pathogenic fungi

Elad

Rav‐David

Leibman

et al. 2012

Annals of Applied Biology

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The inhibitor-of-virus replication (IVR) gene associated with the local lesion response to Tobacco mosaic virus in tobacco codes for a putative protein with a molecular mass of 22 kDa. Earlier work revealed that when tomato (Solanum lycopersicum) cv. VF36 plants are transformed with a cDNA clone encoding this gene, they become partially resistant to Botrytis cinerea infection. The transformation of tomatoes with the IVR gene induced partial resistance to in vitro seedling infection by Alternaria alternata, Pythium aphanidermatum and Rhizoctonia solani and faster seedling growth. Resistance to damping-off was observed in transgenic seedlings planted in soil infested with R. solani and P. aphanidermatum. Early blight (Alternaria solani) and powdery mildew (Oidium neolycopersici) were also partially controlled in mature transgenic tomato plants.

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Overexpression of thaumatin-like protein in transgenic tomato plants confers enhanced resistance toAlternaria solani

Cited by 13 publications

References 33 publications

Effect of formulated zimmu (Allium cepa L. × Allium sativum L.) extract in the management of grain mold of sorghum

Effect of formulated zimmu (Allium cepa L. × Allium sativum L.) extract in the management of grain mold of sorghum

Tomato-Thaumatin-like Protein Genes Solyc08g080660 and Solyc08g080670 Confer Resistance to Five Soil-Borne Diseases by Enhancing β-1,3-Glucanase Activity

Tomato plants transformed with the inhibitor‐of‐virus‐replication gene are partially resistant to several pathogenic fungi

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