Overexpression of sucrose transporter gene PbSUT2 from Pyrus bretschneideri , enhances sucrose content in Solanum lycopersicum fruit

Wang, Lifen; Qi, Xiaoxiao; Huang, Xiaosan; Xu, Li; Jin, Can; Jun, Wu; Zhang, Shaoling

doi:10.1016/j.plaphy.2016.04.019

Cited by 40 publications

(20 citation statements)

References 68 publications

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“…). In previous studies, transgenic plants overexpressing sugar transporters showed similar phenotypes, such as with early flowering of PbSUT2 ‐overexpressing tomato plants (Wang et al ). Although many details of the mechanisms that TMTs act on flowering remain unknown, our sugar transport experiments in PbTMT4‐ overexpressing tomato plants further indicate sugar transport functions of the encoded protein.…”

Section: Discussionmentioning

confidence: 74%

The gene PbTMT4 from pear (Pyrus bretschneideri) mediates vacuolar sugar transport and strongly affects sugar accumulation in fruit

Cheng

Lü

et al. 2018

Physiologia Plantarum

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Tonoplast monosaccharide transporters (TMTs) play important roles in vacuolar sugar accumulation in plants. In this study, six TMT genes (PbTMT1-6) were identified in the Pyrus bretschneideri genome database, and their expression profiles were correlated with soluble sugar contents during the pear (P. bretschneideri cv. Ya Li) fruit development process. Subsequently, PbTMT4 was identified as a strong contributor to fructose, glucose and sucrose accumulation in fructescence of pears. Heterologous expression of PbTMT4, in the hexose transporter-deficient yeast strain EBY.VW4000, facilitated growth in media containing low levels of glucose, fructose, sucrose or sorbitol. In addition, PbTMT4-transformed tomato plants flowered and bore fruit significantly earlier than wild-type (WT) plants, and glucose and fructose levels in mature tomatoes were increased by about 32 and 21% compared with those in WT plants. However, no obvious alterations in sucrose content, plant height and weight per fruit were observed. Finally, subcellular localization experiments in transformed Arabidopsis plants showed that PbTMT4 is localized to tonoplast vesicles of protoplasts. These preliminary results suggest that PbTMT4 participates in vacuolar accumulation of sugars, and thus affects plant growth and development.

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Section: Discussionmentioning

confidence: 74%

The gene PbTMT4 from pear (Pyrus bretschneideri) mediates vacuolar sugar transport and strongly affects sugar accumulation in fruit

Cheng

Lü

et al. 2018

Physiologia Plantarum

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“…In line with this, their overexpression has been found to improve the transport of sucrose to developing sink tissues and at least in certain instances has provided a positive impact on aboveground biomass and/or seed/fruit/tuber yield (e.g. Leggewie et al 2003;Sun et al 2011;Saalbach et al 2014;Wang et al 2015Wang et al , 2016. Similarly, the overexpression of cell wall invertases, which are involved in sucrose cleavage in sink tissues, or the down-regulation of a cell wall invertase inhibitor, can also lead to enhanced sink strength and biomass production or seed yield (e.g.…”

Section: Enhancement Of Alternative Processes To Maintain High Photosmentioning

confidence: 83%

Biotechnological strategies for improved photosynthesis in a future of elevated atmospheric CO2

Singer

Soolanayakanahally

Foroud

et al. 2019

Planta

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“…Like MdSUT4.1, PpSUT4 was shown to reside in the vacuolar membrane by its transient expression in tobacco leaf [29]. However, both MdSUT1 and PbSUT2 were shown to localize in the plasma membrane via their ectopic expression in Arabidopsis protoplasts and onion epidermis cells [33,52]. This inconsistency indicates that other unknown factors, besides the di-leucine motif, may also influence subcellular localization of SUT4 subfamily members.…”

Section: Discussionmentioning

confidence: 99%

“…Sugar accumulation is strongly associated with fruit yield and quality. Since sugar transporter genes play important roles in sugar accumulation [45,46], increasing attention has been paid to investigate SUT gene family over the past two decades in a wide range of fruit species, such as grapevine [47][48][49], sweet orange [50], peach [29,51], pear [52] and apple [53]. A SUT2 gene, i.e.…”

Section: Introductionmentioning

confidence: 99%

The sucrose transporter MdSUT4.1 participates in the regulation of fruit sugar accumulation in apple

et al. 2020

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Background: Sugar content is an important determinant of fruit sweetness, but details on the complex molecular mechanism underlying fruit sugar accumulation remain scarce. Here, we report the role of sucrose transporter (SUT) family in regulating fruit sugar accumulation in apple. Results: Gene-tagged markers were developed to conduct candidate gene-based association study, and an SUT4 member MdSUT4.1 was found to be significantly associated with fruit sugar accumulation. MdSUT4.1 encodes a tonoplast localized protein and its expression level had a negative correlation with fruit sugar content. Overexpression of MdSUT4.1 in strawberry and apple callus had an overall negative impact on sugar accumulation, suggesting that it functions to remobilize sugar out of the vacuole. In addition, MdSUT4.1 is located on chromosomal region harboring a previously reported QTL for sugar content, suggesting that it is a candidate gene for fruit sugar accumulation in apple. Conclusions: MdSUT4.1 is involved in the regulation of fruit sugar accumulation in apple. This study is not only helpful for understanding the complex mechanism of fruit sugar accumulation, but it also provides molecular tools for genetic improvement of fruit quality in breeding programs of apple.

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Overexpression of sucrose transporter gene PbSUT2 from Pyrus bretschneideri , enhances sucrose content in Solanum lycopersicum fruit

Cited by 40 publications

References 68 publications

The gene PbTMT4 from pear (Pyrus bretschneideri) mediates vacuolar sugar transport and strongly affects sugar accumulation in fruit

The gene PbTMT4 from pear (Pyrus bretschneideri) mediates vacuolar sugar transport and strongly affects sugar accumulation in fruit

Biotechnological strategies for improved photosynthesis in a future of elevated atmospheric CO2

The sucrose transporter MdSUT4.1 participates in the regulation of fruit sugar accumulation in apple

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