Biotechnological strategies for improved photosynthesis in a future of elevated atmospheric CO2

Singer, Stacy D.; Soolanayakanahally, Raju; Foroud, Nora A.; Kroebel, R.

doi:10.1007/s00425-019-03301-4

Cited by 15 publications

(14 citation statements)

References 245 publications

(328 reference statements)

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“…The exclusive client of Rca is the photosynthetic key enzyme Rubisco (ribulose-1,5bisphosphate carboxylase/oxygenase), which is directly or indirectly responsible for all biomass production. Since Rca is required for optimal Rubisco function, understanding its mechanism is important in efforts to enhance photosynthesis with the goal to increasing crop yields (Andralojc et al, 2018;Bailey-Serres et al, 2019;Eva et al, 2019;Sharwood, 2017;Singer et al, 2019;Slattery and Ort, 2019).…”

Section: Introductionmentioning

confidence: 99%

Dual Role of a Rubisco Activase in Metabolic Repair and Carboxysome Organization

Hayer‐Hartl

Flecken

Wang

et al. 2020

Preprint

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Rubisco, the key enzyme of CO2 fixation in photosynthesis, is prone to inactivation by inhibitory sugar phosphates. Inhibited Rubisco undergoes conformational repair by the hexameric AAA+ chaperone Rubisco activase (Rca) in a process that is not well understood. Here we performed a structural and mechanistic analysis of cyanobacterial Rca, a close homolog of plant Rca. In the Rca:Rubisco complex, Rca is positioned over the Rubisco catalytic site under repair and pulls the N-terminal tail of the large Rubisco subunit (RbcL) into the hexamer pore. Simultaneous displacement of the C-terminus of the adjacent RbcL opens the catalytic site for inhibitor release.An alternative interaction of Rca with Rubisco is mediated by C-terminal domains that resemble the small Rubisco subunit. These domains, together with the N-terminal AAA+ hexamer, ensure that Rca is packaged with Rubisco into carboxysomes. Cyanobacterial Rca is a dual-purpose protein with functions in Rubisco repair and carboxysome organization.

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Section: Introductionmentioning

confidence: 99%

Dual Role of a Rubisco Activase in Metabolic Repair and Carboxysome Organization

Hayer‐Hartl

Flecken

Wang

et al. 2020

Preprint

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“…Unfortunately, such discrepancies in regulatory requirements between new crop varieties that have been developed using conventional and biotechnological breeding approaches have stifled the commercialization of many transgenic crops due to the exorbitant cost and duration of de-regulation (Macnaghten and Habets, 2020). While a plethora of traits have been improved in crop species to date using a transgenic approach (e.g., Xue et al, 2004;Li, Liu, et al, 2013;Usher et al, 2015;Paul et al, 2017;Singer et al, 2019), the market is dominated by two main transgenic traitsherbicide tolerance (e.g., RoundUp Ready) and/or insect resistance (e.g., through the expression of Cry proteins from Bacillus thuringiensis [Bt]). Neither of these traits were designed explicitly to benefit the consumer, but instead were directed at increasing profits for large producer outfits and seed companies (Macnaghten and Habets, 2020).…”

Section: Breeding Platforms and Crop Regulationmentioning

confidence: 99%

Genetic Variation and Unintended Risk in the Context of Old and New Breeding Techniques

Singer

Laurie

Bilichak

et al. 2021

Critical Reviews in Plant Sciences

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For thousands of years, humans have been improving crops to better suit their needs. These enhancements are driven by changes in the genetic makeup of the plant. While this was initially unintentional, there has been a steady push to increase the pace and precision of crop breeding, something that has occurred alongside a growing understanding of genetics and an escalating capacity to thoroughly assess genomes at the molecular level. With the advent and rapid uptake of molecular breeding techniques, such as transgenics and genome editing over the past few decades, there has been much trepidation regarding the possibility of off-target effects derived from unanticipated mutations at loci other than those intended for alteration, and the unintended risks that this might confer. These concerns persist regardless of the fact that a growing number of studies indicate that the occurrence of off-target mutations derived from newer biotechnological breeding techniques are negligible compared to what is observed with many conventional breeding approaches, and even spontaneously from one generation to the next. Given the impending food security crisis that we are facing in the short-term, there is a critical need to implement a wide range of breeding tools as a means of meeting growing demand, withstanding climate change-related pressures, increasing nutrition, and providing environmental benefits. While food safety is clearly of the utmost importance, now is certainly not the time to prevent the use of particular breeding technologies based on unfounded doubts. Therefore, in this review, we attempt to shed light on these apprehensions by putting purported "risks" into the context of plant breeding as a whole by comparing frequencies of spontaneous mutations with those (both anticipated and unanticipated) that occur through various conventional and biotechnological breeding approaches, including transgenics and genome editing. We then consider how these changes may, or may not, translate into unanticipated risk, and discuss the current global regulatory asynchrony surrounding genome edited crops.

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“…9, No. 3 potential (Orr et al, 2017;van Iersel, 2017;Nowicka et al, 2018;Kaiser et al, 2019;Batista-Silva et al, 2020;Singer et al, 2020). This can be done using different strategies including genetic techniques like the use of genetically modified organisms (Simkin et al, 2019), management of soil microbiota (Silva et al, 2021), or the addition of yield factors such as light (Goto, 2003;Gupta, 2017).…”

Section: Journal Of Agricultural Studiesmentioning

confidence: 99%

Improving Soybean Production Using Light Supplementation at Field-Scale: A Case Study

Lemes¹,

Azevedo²,

Domiciano³

et al. 2021

JAS

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In modern agriculture, there is a growing need for increasing crop efficiency while minimizing environmental impacts. The use of high-efficiency light supplementation to enhance plant development is limited for high-productive crops at field conditions (outdoor). This study evaluated the soybean plant’s yield responses in an open commercial area (field scale) cultivated under conditions of artificial light supplementation. A commercial irrigated (pivot) area received an illumination system for light supplementation (LS) in its inner pivot spans. About 40 hours of LS were applied to the plants during the soybean crop cycle. The area’s outer pivot spans did not receive light supplementation (nLS). The internode number, the plant height, the pods per plant were evaluated weekly to compute the area under the progress curve (AUPC). The grain yield at harvest was also assessed. The AUPC of the internode number, plant height and pods per plant were positively affected by the LS treatment. The regular soybean cycle (nLS) is about 17 weeks; however, the LS harvest occurred three weeks later. Light supplementation increased soybean grain yield by 57.3% and profitability by 180% when compared to nLS. Although light supplementation at field scale poses a challenge, it is now affordable since sustainable field resistant technologies are now available. The present study is the first known report of light supplementation used to improve soybean crop production at field scale.

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Biotechnological strategies for improved photosynthesis in a future of elevated atmospheric CO2

Abstract: Main conclusion The improvement of photosynthesis using biotechnological approaches has been the focus of much research. It is now vital that these strategies be assessed under future atmospheric conditions.

Cited by 15 publications

References 245 publications

Dual Role of a Rubisco Activase in Metabolic Repair and Carboxysome Organization

Dual Role of a Rubisco Activase in Metabolic Repair and Carboxysome Organization

Genetic Variation and Unintended Risk in the Context of Old and New Breeding Techniques

Improving Soybean Production Using Light Supplementation at Field-Scale: A Case Study

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