Overexpression of Sorghum WINL1 gene confers drought tolerance in Arabidopsis thaliana through the regulation of cuticular biosynthesis

Bao, Shuguang; Shi, Jiannong; Luo, Feng; Ding, Bo; Hao, Jianwei; Xie, Xiaodong; Sun, Shi

doi:10.1007/s11240-016-1114-2

Cited by 21 publications

(5 citation statements)

References 60 publications

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“…It is used as an indicator of the level of photosynthetic efficiency, which is significantly lower in sorghum grown under drought stress conditions (Johnson et al 2014 ). Drought stress affects photosynthetic rate in sorghum by decreasing stomatal conductance and transpiration rate (Zhang et al 2019b ), quantum yield and increasing leaf temperature (Kapanigowda et al 2014 ), reduction in chlorophyll and Rubisco, increase in O 2 evolution and decrease in PEPCase activity (Bao et al 2017 ). Different studies have shown that, under drought stress conditions, tolerant sorghum genotypes have significantly higher values of Fv/Fm and photosynthetic rate (Fracasso et al 2016 ; Sukumaran et al 2016 ).…”

Section: Mechanisms Of Drought Tolerance In Sorghummentioning

confidence: 99%

Sorghum in dryland: morphological, physiological, and molecular responses of sorghum under drought stress

Abreha

Enyew

Carlsson

et al. 2021

Planta

102

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Main conclusion Droughts negatively affect sorghum’s productivity and nutritional quality. Across its diversity centers, however, there exist resilient genotypes that function differently under drought stress at various levels, including molecular and physiological. Abstract Sorghum is an economically important and a staple food crop for over half a billion people in developing countries, mostly in arid and semi-arid regions where drought stress is a major limiting factor. Although sorghum is generally considered tolerant, drought stress still significantly hampers its productivity and nutritional quality across its major cultivation areas. Hence, understanding both the effects of the stress and plant response is indispensable for improving drought tolerance of the crop. This review aimed at enhancing our understanding and provide more insights on drought tolerance in sorghum as a contribution to the development of climate resilient sorghum cultivars. We summarized findings on the effects of drought on the growth and development of sorghum including osmotic potential that impedes germination process and embryonic structures, photosynthetic rates, and imbalance in source-sink relations that in turn affect seed filling often manifested in the form of substantial reduction in grain yield and quality. Mechanisms of sorghum response to drought-stress involving morphological, physiological, and molecular alterations are presented. We highlighted the current understanding about the genetic basis of drought tolerance in sorghum, which is important for maximizing utilization of its germplasm for development of improved cultivars. Furthermore, we discussed interactions of drought with other abiotic stresses and biotic factors, which may increase the vulnerability of the crop or enhance its tolerance to drought stress. Based on the research reviewed in this article, it appears possible to develop locally adapted cultivars of sorghum that are drought tolerant and nutrient rich using modern plant breeding techniques.

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Section: Mechanisms Of Drought Tolerance In Sorghummentioning

confidence: 99%

Sorghum in dryland: morphological, physiological, and molecular responses of sorghum under drought stress

Abreha

Enyew

Carlsson

et al. 2021

Planta

102

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“…Overexpression of maize ZmDBP3 enhances tolerance of transgenic Arabidopsis to drought and cold stresses [21]. In contrast, overexpression of the WIN1 gene from Sorghum confers drought resistance to Arabidopsis by regulating the epidermis [22]. The ERF and DREB families, in particular, contain members that have excellent performance in response to abiotic stress.…”

Section: Introductionmentioning

confidence: 99%

Genome-wide identification and expression analysis of AP2/ERF transcription factors in sugarcane (Saccharum spontaneum L.)

Chai

Lin

et al. 2020

BMC Genomics

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Background APETALA2/ETHYLENE RESPONSIVE FACTOR (AP2/ERF) transcription factors play essential roles in plant growth, development, metabolism, and responses to biotic and abiotic stresses. However, few studies concerning AP2/ERF genes in sugarcane which are the most critical sugar and energy crops worldwide. Results A total of 218 AP2/ERF genes were identified in the Saccharum spontaneum genome. Phylogenetic analysis showed that these genes could be divided into four groups, including 43 AP2s, 160 ERFs and Dehydration-responsive element-binding (DREB) factors, 11 ABI3/VPs (RAV), and four Soloist genes. These genes were unevenly distributed on 32 chromosomes. The structural analysis of SsAP2/ERF genes showed that 91 SsAP2/ERFs lacked introns. Sugarcane and sorghum had a collinear relationship between 168 SsAP2/ERF genes and sorghum AP2/ERF genes that reflected their similarity. Multiple cis-regulatory elements (CREs) present in the SsAP2/ERF promoter were related to abiotic stresses, suggesting that SsAP2/ERF activity could contribute to sugarcane adaptation to environmental changes. The tissue-specific analysis showed spatiotemporal expression of SsAP2/ERF in the stems and leaves of sugarcane at different development stages. In ten sugarcane samples, 39 SsAP2/ERFs were not expressed, whereas 58 SsAP2/ERFs were expressed in all samples. Quantitative PCR experiments showed that SsERF52 expression was up-regulated under salt stress, but suppressed under dehydration stress. SsSoloist4 had the most considerable upregulation in response to treatment with the exogenous hormones ABA and GA. Within 3 h of ABA or PEG6000 treatment, SsSoloist4 expression was up-regulated, indicating that this gene could play a role in the responses to ABA and GA-associated dehydration stress. Analysis of AP2/ERF gene expression patterns under different treatments indicated that SsAP2/ERF genes played an essential role in dehydration and salt stress responses of S. spontaneum. Conclusions In this study, a total of 218 members of the AP2 / ERF superfamily were identified in sugarcane, and their genetic structure, evolution characteristics, and expression patterns were studied and analyzed. The results of this study provide a foundation for future analyses to elucidate the importance of AP2/ERF transcription factors in the function and molecular breeding of sugarcane.

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“…Overexpression of maize ZmDBP3 enhances tolerance of transgenic Arabidopsis to drought and cold stress [22]. In contrast, overexpression of the WIN 1 gene from sorghum confers drought resistance to Arabidopsis by regulating the biosynthesis of the epidermis [23]. The ERF and DREB families, in particular, contain members that have excellent performance in response to abiotic stress.…”

Section: Introductionmentioning

confidence: 99%

Genome-wide analysis of AP2/ERF Transcription Factors in sugarcane Saccharum spontaneum L. Reveals Functional Divergence During Drought, Salt Stress and Plant Hormones Treatment

Chai

Lin

et al. 2020

Preprint

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Background: APETALA2/ETHYLENE RESPONSIVE FACTOR (AP2/ERF) transcription factors play important roles in plant growth, development, metabolism, as well as in biotic and abiotic stress responses. However, there are few studies concerning AP2/ERF genes in sugarcane, which is the most critical sugar and energy crop worldwide. Results: A total of 218 AP2/ERF genes were identified in the Saccharum spontaneum genome. Phylogenetic analysis showed that these genes could be divided into four groups, including 43 AP2s, 160 ERFs, and Dehydration-responsive element-binding (DREB) factors, 11 ABI3/VPs (RAV) and 4 Soloist genes. These genes were unevenly distributed on 32 chromosomes. Analysis of the structural of SsAP2/ERF genes showed that 91 SsAP2/ERFs lacked introns. Sugarcane and sorghum have a collinear relationship between 168 SsAP2/ERF genes and sorghum AP2/ERF genes that reflects their similarity. Multiple cis-regulatory elements (CREs) are present in the SsAP2/ERF promoter, and many are related to abiotic stresses, suggesting that SsAP2/ERF activity could contribute to the adaptation of sugarcane crops to environmental changes. The tissue-specific analysis showed spatiotemporal expression of SsAP2/ERF in the stems and leaves of sugarcane at different stages of development. In 10 sugarcane samples, 39 SsAP2/ERFs were not expressed at all, whereas 58 SsAP2/ERFs were expressed in all samples. Quantitative PCR experiments showed that SsERF52 expression was up-regulated under salt stress, but suppressed under drought stress. SsSoloist4 had the most considerable upregulation in response to treatment with the exogenous hormones ABA and GA. Within 3 hours of ABA or PEG6000 treatment, SsSoloist4 expression was up-regulated, indicating that this gene could play a role in ABA and GA-associated drought stress response mechanisms. Analysis of AP2/ERF gene expression patterns under different treatments indicated that SsAP2/ERF genes play an important role in drought and salt stress responses of S. spontaneum. Conclusions: In this study, a total of 218 members of the AP2 / ERF superfamily were identified in sugarcane, and their genetic structure, evolution characteristics, and expression patterns were studied and analyzed. The results of this study provide a foundation for future analyses to elucidate the importance of AP2/ERF transcription factors in the function and molecular breeding of sugarcane.

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Overexpression of Sorghum WINL1 gene confers drought tolerance in Arabidopsis thaliana through the regulation of cuticular biosynthesis

Cited by 21 publications

References 60 publications

Sorghum in dryland: morphological, physiological, and molecular responses of sorghum under drought stress

Sorghum in dryland: morphological, physiological, and molecular responses of sorghum under drought stress

Genome-wide identification and expression analysis of AP2/ERF transcription factors in sugarcane (Saccharum spontaneum L.)

Genome-wide analysis of AP2/ERF Transcription Factors in sugarcane Saccharum spontaneum L. Reveals Functional Divergence During Drought, Salt Stress and Plant Hormones Treatment

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