Overexpression of Shinorhizobium meliloti flavohemoglobin improves cell growth and fatty acid biosynthesis in oleaginous fungus Mucor circinelloides

Zhang, Huaiyuan; Kang, Xiaoning; Xin, Feifei; Chang, Yufei; Zhang, Yingtong; Song, Yuanda

doi:10.1007/s10529-022-03242-1

Cited by 2 publications

(3 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Huaiyuan Zhang et al introduced the vgb gene into Mucor circinelloides and found that it could alleviate oxygen limitation, thereby promoting cell growth and fatty acid synthesis [28]. Jun-feng Li et al transformed the vgb gene into S. bombicola O-13-1 and found that expression of the gene increased the efficiency of intracellular oxygen utilization under oxygen-enriched or oxygen-limited conditions [29].…”

Section: Discussionmentioning

confidence: 99%

Improved Recombinant Expression of Maltogenic α-Amylase AmyM in Bacillus subtilis by Optimizing Its Secretion and NADPH Production

et al. 2023

View full text Add to dashboard Cite

The maltose α-amylase AmyM from Bacillus stearothermophilus can be used for flour modification, baked goods preservation, and maltose production. Here, we optimized the recombinant expression of AmyM in Bacillus subtilis WB800 via several strategies. By screening the optimal promoter, a double promoter combination (P43 and PamyL) could improve the expression level of AmyM by 61.25%, compared with the strong promoter P43. Then, we optimized the secretion efficiency of recombinant AmyM by over-expressing the molecular chaperone prsA gene. SDS-PAGE results suggested that over-expression of the prsA could improve the secretion efficiency of AmyM to the extracellular environment. The extracellular enzyme activity of AmyM was increased by 101.58% compared to the control strain. To further improve the expression of AmyM, we introduced the hemoglobin gene of Vitreoscilla (vgb) into the AmyM recombinant strain. The results revealed that the introduction of vgb could promote the transcription and translation of AmyM in B. subtilis. This may be due to the increasing level of intracellular NADPH and NADP+ caused by the expression of vgb. By this strategy, the expression level of AmyM was increased by 204.08%. Finally, we found the recombinant AmyM showed an optimal temperature of 65 °C and an optimal pH of 5.5. Our present results provided an effective strategy for increasing the heterologous expression level of AmyM in B. subtilis.

show abstract

Section: Discussionmentioning

confidence: 99%

Improved Recombinant Expression of Maltogenic α-Amylase AmyM in Bacillus subtilis by Optimizing Its Secretion and NADPH Production

et al. 2023

View full text Add to dashboard Cite

show abstract

“…In contrast to VHb, SHb has a more complex structure with additional highly conserved oxidoreductase, FAD- and NAD(P)-binding domains, enhancing the supply of NADPH. Notably, SHb improved the yield of ARA mainly by affecting the total lipid content (3.09-fold increase compared to the control) …”

Section: Metabolic Engineering Strategies For Enhancing Ara Accumulationmentioning

confidence: 98%

“…Notably, SHb improved the yield of ARA mainly by affecting the total lipid content (3.09-fold increase compared to the control). 46 3.4. Introducing a Heterologous PKS Pathway.…”

Section: Improving the Efficiency Of Cellular Oxygen Utilizationmentioning

confidence: 99%

Recent Development of Advanced Biotechnology in the Oleaginous Fungi for Arachidonic Acid Production

Guo

Peng

et al. 2022

ACS Synth. Biol.

View full text Add to dashboard Cite

Arachidonic acid is an essential ω-6 polyunsaturated fatty acid, which plays a significant role in cardiovascular health and neurological development, leading to its wide use in the food and pharmaceutical industries. Traditionally, ARA is obtained from deep-sea fish oil. However, this source is limited by season and is depleting the already threatened global fish stocks. With the rapid development of synthetic biology in recent years, oleaginous fungi have gradually attracted increasing attention as promising microbial sources for large-scale ARA production. Numerous advanced technologies including metabolic engineering, dynamic regulation of fermentation conditions, and multiomics analysis were successfully adapted to increase ARA synthesis. This review summarizes recent advances in the bioengineering of oleaginous fungi for ARA production. Finally, perspectives for future engineering approaches are proposed to further improve the titer yield and productivity of ARA.

show abstract

Overexpression of Shinorhizobium meliloti flavohemoglobin improves cell growth and fatty acid biosynthesis in oleaginous fungus Mucor circinelloides

Cited by 2 publications

References 35 publications

Improved Recombinant Expression of Maltogenic α-Amylase AmyM in Bacillus subtilis by Optimizing Its Secretion and NADPH Production

Improved Recombinant Expression of Maltogenic α-Amylase AmyM in Bacillus subtilis by Optimizing Its Secretion and NADPH Production

Recent Development of Advanced Biotechnology in the Oleaginous Fungi for Arachidonic Acid Production

Contact Info

Product

Resources

About