Overexpression of Rosa rugosa anthocyanidin reductase enhances tobacco tolerance to abiotic stress through increased ROS scavenging and modulation of ABA signaling

Luo, Ping; Shen, Yuxiao; Jin, Shuangxia; Shasha, Huang; Xu, Cheng; Wang, Zhen; Li, Penghui; Zhao, Jian; Bao, Manzhu; Ning, Guogui

doi:10.1016/j.plantsci.2016.01.007

Cited by 62 publications

(38 citation statements)

References 59 publications

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“…Flavonoid-related proteins were down-regulated in 2 ry lines, which may explain their higher embryogenic potential. These flavonoids play important roles in the cells since they have several links with oxidative stress responses [ 79 , 80 ]. Flavonoids are polyphenols (compounds derived from phenylpropanoids), and phenylalanine ammonia-lyase (PAL) is the first enzyme involved in their committed synthesis from the amino acid phenylalanine.…”

Section: Discussionmentioning

confidence: 99%

Repetitive somatic embryogenesis induced cytological and proteomic changes in embryogenic lines of Pseudotsuga menziesii [Mirb.]

et al. 2018

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BackgroundTo explore poorly understood differences between primary and subsequent somatic embryogenic lines of plants, we induced secondary (2ry) and tertiary (3ry) lines from cotyledonary somatic embryos (SEs) of two Douglas-fir genotypes: SD4 and TD17. The 2ry lines exhibited significantly higher embryogenic potential (SE yields) than the 1ry lines initiated from zygotic embryos (SD4, 2155 vs 477; TD17, 240 vs 29 g− 1 f.w.). Moreover, we observed similar differences in yield between 2ry and 3ry lines of SD4 (2400 vs 3921 g− 1 f.w.). To elucidate reasons for differences in embryogenic potential induced by repetitive somatic embryogenesis we then compared 2ry vs 1ry and 2ry vs 3ry lines at histo-cytological (using LC-MS/MS) and proteomic levels.ResultsRepetitive somatic embryogenesis dramatically improved the proliferating lines’ cellular organization (genotype SD4’s most strongly). Frequencies of singulated, bipolar SEs and compact polyembryogenic centers with elongated suspensors and apparently cleavable embryonal heads increased in 2ry and (even more) 3ry lines. Among 2300–2500 identified proteins, 162 and 228 were classified significantly differentially expressed between 2ry vs 1ry and 3ry vs 2ry lines, respectively, with special emphasis on “Proteolysis” and “Catabolic process” Gene Ontology categories. Strikingly, most of the significant proteins (> 70%) were down-regulated in 2ry relative to 1ry lines, but up-regulated in 3ry relative to 2ry lines, revealing a down-up pattern of expression. GO category enrichment analyses highlighted the opposite adjustments of global protein patterns, particularly for processes involved in chitin catabolism, lignin and L-phenylalanine metabolism, phenylpropanoid biosynthesis, oxidation-reduction, and response to karrikin. Sub-Network Enrichment Analyses highlighted interactions between significant proteins and both plant growth regulators and secondary metabolites after first (especially jasmonic acid, flavonoids) and second (especially salicylic acid, abscisic acid, lignin) embryogenesis cycles. Protein networks established after each induction affected the same “Plant development” and “Defense response” biological processes, but most strongly after the third cycle, which could explain the top embryogenic performance of 3ry lines.ConclusionsThis first report of cellular and molecular changes after repetitive somatic embryogenesis in conifers shows that each cycle enhanced the structure and singularization of EMs through modulation of growth regulator pathways, thereby improving the lines’ embryogenic status.Electronic supplementary materialThe online version of this article (10.1186/s12870-018-1337-y) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Repetitive somatic embryogenesis induced cytological and proteomic changes in embryogenic lines of Pseudotsuga menziesii [Mirb.]

et al. 2018

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“…In general, to adapt to stresses, plants activate series physiological and biochemical responses that are operated by complex signaling networks (Ha et al 2014a;Ha et al 2013a;Mochida et al 2015;Osakabe et al 2014;Yamaguchi-Shinozaki and Shinozaki 2006). Under drought stress, plants increase guard cell closure to maintain water status, and enhance activity of antioxidant enzymes to maintain ROS levels in plant cells (Luo et al 2016;Nguyen et al 2018b). To determine the potential role of nano-Cu o in ROS homeostasis, we examined the ROS production such as O2 •− and H2O2 in the maize leaf of nano-Cu o -treated and control plants under normal and drought stress conditions.…”

Section: Nano-cu O Priming Decreases Ros Accumulation In Maize Under mentioning

confidence: 99%

Copper nanoparticle application enhances plant growth and grain yield in maize under drought stress conditions

Nguyen

et al. 2020

Preprint

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Abiotic stresses, including drought, detrimentally affect the growth and productivity of many economically important crop plants, leading to significant yield losses, which can result in food shortages and threaten the sustainability of agriculture. Balancing between plant growth and stress responses is one of the most important characters for agricultural application to maximize plant production. In this study, we initially report that copper nanoparticle priming positively regulates drought stress responses in maize. The copper nanoparticle priming plants displayed enhanced drought tolerance indicated by their higher leaf water content and plant biomass under drought as compared with water-treated plants. Moreover, our data showed that the treatment of copper nanoparticle on plants increased anthocyanin, chlorophyll and carotenoid contents compared to water-treated plants under drought stress conditions. Additionally, histochemical analyses with nitro blue tetrazolium and 3,3'-diaminobenzidine revealed that reactive oxygen species accumulation of priming plants was decreased as a result of enhancement of reactive oxygen species scavenging enzyme activities under drought. Furthermore, our comparative yield analysis data indicated applying copper nanoparticle to plant increased total seed number and grain yield under drought stress conditions. Our data provided the evidences that copper nanoparticle regulates plant protective mechanisms associated with drought tolerance, which is a promising approach for the production of drought tolerant crop plants.

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“…Furthermore, oxidative stress can induce PA biosynthesis and PA content including spermidin and spermine (Smirnova et al 2012). Some reports have revealed that overexpression of SAMS gene can enhance the tolerance of tobacco against abiotic stress and increase the PA content in tobacco (Luo et al 2016).…”

Section: Discussionmentioning

confidence: 99%

Characterization of S-adenosylmethionine Synthetase Gene from Red Algae, Pyropia tenera

Han

Hong

Han

et al. 2016

Plant Breed. Biotech.

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S-adenosylmethionine (SAM) is widely involved in variety of biosynthetic processes. It participates in the methionine (Met) metabolic pathway. SAM synthetase (EC 2.5.1.6) is the main enzyme that creates SAM from Met and adenosine triphosphate. SAMS genes are highly conserved in different species. We have isolated SAMS genes from red algae, Pyropia tenera. It has been reported that SAMS genes can confer abiotic stress-related response and growth of plant organs in other species. To elucidate the function of PtSAMS gene in evolutionary perspective, we analyzed SAMS gene using bioinformatics tools and plant transformation of Arabidopsis.

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Overexpression of Rosa rugosa anthocyanidin reductase enhances tobacco tolerance to abiotic stress through increased ROS scavenging and modulation of ABA signaling

Cited by 62 publications

References 59 publications

Repetitive somatic embryogenesis induced cytological and proteomic changes in embryogenic lines of Pseudotsuga menziesii [Mirb.]

Repetitive somatic embryogenesis induced cytological and proteomic changes in embryogenic lines of Pseudotsuga menziesii [Mirb.]

Copper nanoparticle application enhances plant growth and grain yield in maize under drought stress conditions

Characterization of S-adenosylmethionine Synthetase Gene from Red Algae, Pyropia tenera

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