2018
DOI: 10.1186/s12870-018-1337-y
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Repetitive somatic embryogenesis induced cytological and proteomic changes in embryogenic lines of Pseudotsuga menziesii [Mirb.]

Abstract: BackgroundTo explore poorly understood differences between primary and subsequent somatic embryogenic lines of plants, we induced secondary (2ry) and tertiary (3ry) lines from cotyledonary somatic embryos (SEs) of two Douglas-fir genotypes: SD4 and TD17. The 2ry lines exhibited significantly higher embryogenic potential (SE yields) than the 1ry lines initiated from zygotic embryos (SD4, 2155 vs 477; TD17, 240 vs 29 g− 1 f.w.). Moreover, we observed similar differences in yield between 2ry and 3ry lines of SD4 … Show more

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Cited by 20 publications
(21 citation statements)
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“…Embryogenic cultures of gymnosperms proliferate as EMs that typically have a whitish to translucent appearance and may have a granular to spiky (SK) morphotype due to early embryos protruding at their surface (see Gautier et al, 2018 and references therein). Modifications in water availability, either by solute-imposed water stress or by physical restriction, will impact the development of embryogenic tissue (Klimaszewska et al, 2000).…”
Section: Introductionmentioning
confidence: 99%
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“…Embryogenic cultures of gymnosperms proliferate as EMs that typically have a whitish to translucent appearance and may have a granular to spiky (SK) morphotype due to early embryos protruding at their surface (see Gautier et al, 2018 and references therein). Modifications in water availability, either by solute-imposed water stress or by physical restriction, will impact the development of embryogenic tissue (Klimaszewska et al, 2000).…”
Section: Introductionmentioning
confidence: 99%
“…In different Picea glauca x engelmanni genotypes the ability of embryogenic tissue to utilize ABA from the medium may reflect their maturation capability (Kong and von Aderkas, 2007). The switch from EMs proliferation to somatic embryo development and maturation has recently been investigated by both transcriptome and proteome profiling (Morel et al, 2014a; Plomion et al, 2016; Trontin et al, 2016; Klubicová et al, 2017; Gautier et al, 2018). In maritime pine, yield and quality of mature somatic embryos is strongly dependent on the embryogenic line (Lelu-Walter et al, 2016; Cano et al, 2018).…”
Section: Introductionmentioning
confidence: 99%
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“…The ubiquitin protein ligase plays a major role as a robust marker of correct embryo development in Pinus pinaste [26] and the somatic embryogenic lines of Pseudotsuga menziesii [Mirb.] [27]. In our study, the sister chromatid cohesion protein PDS5 homolog A isoform X2 was specifically expressed in the globular, torpedo and plantlet stages that regulate chromosome segregation during mitosis and meiosis and was also found to play a role in DNA repair.…”
Section: Discussionmentioning
confidence: 61%
“…Despite similar embryo productivity, i.e., around 120 embryos per g FW in the original EM lines (derived from the zygotic embryos), the EM sublines of two responsive Norway spruce genotypes grew very differently after initiation. This may be due to differentially expressed proteins like in secondary and tertiary lines of Douglas-fir ( Gautier et al, 2018 ). The EM sublines of 11Pa3794 had much lower embryo productivity than sublines of 11Pa4066, possibly due to the untimely subculture, or other factors.…”
Section: Discussionmentioning
confidence: 99%