Overexpression of PaFT gene in the wild orchid Phalaenopsis amabilis (L.) Blume

Semiarti, Endang; Mercuriani, Ixora Sartika; Rizal, Rinaldi; Slamet, Agus; Utami, Sri; Bestari, Ida Ayu Purnama; Aziz-Purwantoro,; Moeljopawiro, Sukarti; Jang, Soenghoe; Machida, Yasunori; Machida, Chiyoko

doi:10.1063/1.4930750

Cited by 6 publications

(2 citation statements)

References 6 publications

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“…To accelerate the floral transition and shorten the reproductive cycle of Phalaenopsis, transformants were obtained by overexpression of FT (Flowering locus T), a floral transition-related gene derived from Phal. amabilis by AT method [73,74]. Overexpression of FT is known to be involved in early flowering by promoting floral transition in Arabidopsis thaliana and other species.…”

Section: Flower Traitsmentioning

confidence: 99%

Applications of Biotechnological Approaches in the Product and Breeding of Phalaenopsis Orchids

Enoki¹,

Takahara²

2023

Tropical Plant Species and Technological Interventions for Improvement

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Phalaenopsis orchids native to the tropics are called “Moth Orchids”. It is one of the most commercially popular orchids because of its beautiful, colorful, and long-lasting variety of flowers. Biotechnology used in the production and breeding of Phalaenopsis was reviewed in this chapter. In the commercial production of Phalaenopsis, biotechnologies, such as methods of aseptic sowing and tissue culture, have been used for a long time. Recently, molecular phylogenetic analysis of original species and molecular breeding by the transformation of Phalaenopsis has been actively studied. The role of biotechnology in the Phalaenopsis orchid industry is significant, and the development of the technology in this field will bring further benefits to researchers, producers, and fancier of Phalaenopsis orchids.

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Section: Flower Traitsmentioning

confidence: 99%

Applications of Biotechnological Approaches in the Product and Breeding of Phalaenopsis Orchids

Enoki¹,

Takahara²

2023

Tropical Plant Species and Technological Interventions for Improvement

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“…The transition phase from vegetative to flowering in Arabidopsis thaliana starts from the activity of the CONSTAN gene inducing the formation of the FT (Flowering Time locus-T) protein with FD (Flowering Time locus-D) to increase the LEAFY and APETALA genes which are genes that regulate the apical stem meristem into a flower meristem (Turck et al, 2008). Research related to flowering using PaFT gene expression has been carried out by Semiarti et al (2015) by overexpressing the PaFT gene in P. amabilis orchid plants which can cause the expression of the POH1 gene (vegetative phase coding gene) to decrease so that the vegetative phase can be shortened (Semiarti et al, 2015). However, the presence of the GAI (Gibberellic Acid Insensitive) gene as an inhibitor can inhibit the regulation of the PaFT gene (Ding et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Identification of early flowering mutant gene in Phalaenopsis amabilis (L.) Blume for sgRNA construction in CRISPR/Cas9 genome editing system

et al. 2024

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Phalaenopsis amabilis (L.) Blume commonly called Moth Orchid (Orchidaceae) is a natural orchid species designated as the National Flower of Indonesia for its beautiful flower shape and long-lasting flowering period. Basically, P. amabilis has a long vegetative phase that cause late flowering, about 2 to 3 years for flowering, hence a method to shorten vegetative period is desired. The latest technological approach that can be used to accelerate flowering of P. amabilis is the CRISPR/Cas9 genome editing method to inactivate the GAI (Gibberellic Acid Insensitive) gene as a mutant gene that can accelerate the regulation of FLOWERING TIME (FT) genes flowering biosynthesis pathway. The approach that needs to be taken is to silence the GAI gene with a knockout system which begins with identifying and characterizing the GAI target gene in the P. amabilis which will be used as a single guide RNA. CRISPR/Cas9 mediated knockout efficiency is highly dependent on the properties of the sgRNA used. SgRNA consists of a target sequence, determining its specificity performance. We executed phylogenetic clustering for the PaGAI protein with closely related orchid species such as Dendrobium capra, Dendrobium cultivars and Cymbidium sinensis. SWISS-Model as tool webserver for protein structure homology modeling. Results show that P. amabilis has a specific domain with the occurrence of point mutations in the two conservative domains. Therefore, a single guide RNA reconstruction needs to be implemented.

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Dynamic expression of POH1 gene in shoot development during in vitro culture of Phalaenopsis orchid

Semiarti

Slamet

Rizal

et al. 2016

AIP Conference Proceedings

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Overexpression of PaFT gene in the wild orchid Phalaenopsis amabilis (L.) Blume

Cited by 6 publications

References 6 publications

Applications of Biotechnological Approaches in the Product and Breeding of Phalaenopsis Orchids

Applications of Biotechnological Approaches in the Product and Breeding of Phalaenopsis Orchids

Identification of early flowering mutant gene in Phalaenopsis amabilis (L.) Blume for sgRNA construction in CRISPR/Cas9 genome editing system

Dynamic expression of POH1 gene in shoot development during in vitro culture of Phalaenopsis orchid

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