Overexpression of OsERF1, a novel rice ERF gene, up-regulates ethylene-responsive genes expression besides affects growth and development in Arabidopsis

Hu, Yibing; Zhao, Lei; Chong, Kang; Wang, Tai

doi:10.1016/j.jplph.2007.12.006

Cited by 68 publications

(45 citation statements)

References 29 publications

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“…In this study, functional validation of AP2/ERF-N22 driven under a 35S constitutive promoter in Arabidopsis showed phenotypic aberrations, very much as in other similar reports (Romero et al 1997;Yusuke et al 2006, Hu et al 2008Morran et al 2011;Chan et al 2012;Gargul et al 2013;Nicky et al 2013). Previous studies have indicated that the AP2 family TFs, which specifically bind to the DRE/CRT or the GCC box elements, play major roles in tolerance to biotic and abiotic stresses by regulating the expression of stress-responsive genes either through the ABA-independent or ABA-dependent pathways (Krizek 2011;Soares et al 2011;Cheng et al 2013).…”

Section: Discussionsupporting

confidence: 86%

Functional validation of a drought-responsive AP2/ERF family transcription factor-encoding gene from rice in Arabidopsis

et al. 2015

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A water-deficit stress (WDS)-responsive transcription factor-encoding gene isolated from drought-tolerant Oryza sativa L. cv. N-22 corresponding to the AP2/ERF family and named AP2/ERF-N22, when overexpressed in Arabidopsis under a constitutive promoter, showed improved turgor and less wilting as compared to wild-type plants under WDS. However, the transgenic plants showed phenotypic aberrations such as stunted growth, smaller silique size, reduced number of seeds and delayed flowering. Physio-biochemical analysis of T2 plants revealed higher relative water content, membrane stability index, capacity to scavenge reactive oxygen species and increased levels of proline, carotenoids, wax and abscisic acid content under WDS. Higher abscisic acid content resulted in reduced stomatal conductance and transpiration rate. The reduced chlorophyll content also leads to reduced photosynthetic rate and efficiency. Thus, this gene may be exploited for enhanced drought tolerance in crop plants.

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Section: Discussionsupporting

confidence: 86%

Functional validation of a drought-responsive AP2/ERF family transcription factor-encoding gene from rice in Arabidopsis

et al. 2015

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“…Interestingly, besides the well-characterized features such as up-regulating ethyleneresponsive genes expression (Solano et al 1998;Park et al 2001) and enhancing pathogen resistance of plants (Zhou et al 1997;Park et al 2001;Berrocal-Lobo et al 2002;Gu et al 2002;Gutterson and Reuber 2004); many ERFs produce remarkable aberrances when overexpressed in plants. As we identified previously that OsERF1, a novel ERF gene of rice, conferred detrimental effects when overexpressed in Arabidopsis (Hu et al 2008). In fact, a similar phenomenon has been reported by Solano et al (1998) in characterizing ERF1 of Arabidopsis, as well as by Kieber et al (1993) in analyzing the CTR1 (constitutive triple response 1), a negative component gene in the ethylene signaling pathway in Arabidopsis.…”

Section: Introductionsupporting

confidence: 79%

Light restored root growth of Arabidopsis with constitutive ethylene response

Chang

et al. 2010

Acta Physiol Plant

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Ethylene response factor (ERF) is an important component in ethylene or pathogen-induced defensive response of plants. However, physiological effects of ERF on plants have not been fully elucidated. We previously identified an ERF gene, OsERF1, in rice. It up-regulated ethylene-responsive genes expression and influenced growth and development of the transgenic Arabidopsis. Here, we report that similar to other seedlings with constitutive ethylene response, OsERF1 seedlings were suppressed in their root growth. Interestingly, the suppressed root growth was restorable by light irradiation. Detailed analysis showed that OsERF1 inhibited cell elongation without influencing cell number in hypocotyls and leaves of the transgenic Arabidopsis. In addition, homozygous OsERF1 was fatal and heterozygous OsERF1 was harmful in Arabidopsis. These findings expand our understanding of ERF.

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“…The AtERF genes were differentially regulated by ethylene and abiotic stress conditions via ethyleneinsensitive2 (EIN2)-dependent or EIN2-independent pathways (Solano et al, 1998;Fujimoto et al, 2000;Nakano et al, 2006). Overexpression of rice OsERF1 in Arabidopsis resulted in up-regulation of the expression of two known ER genes, plant defensin (low-molecularweight Cys-rich 77) and B-chitinase (Hu et al, 2008).…”

Section: Ethylene Signal Transduction-related Genesmentioning

confidence: 99%

Microarray Analysis of the Abscission-Related Transcriptome in the Tomato Flower Abscission Zone in Response to Auxin Depletion

et al. 2010

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The abscission process is initiated by changes in the auxin gradient across the abscission zone (AZ) and is triggered by ethylene. Although changes in gene expression have been correlated with the ethylene-mediated execution of abscission, there is almost no information on the molecular and biochemical basis of the increased AZ sensitivity to ethylene. We examined transcriptome changes in the tomato (Solanum lycopersicum 'Shiran 1335') flower AZ during the rapid acquisition of ethylene sensitivity following flower removal, which depletes the AZ from auxin, with or without preexposure to 1-methylcyclopropene or application of indole-3-acetic acid after flower removal. Microarray analysis using the Affymetrix Tomato GeneChip revealed changes in expression, occurring prior to and during pedicel abscission, of many genes with possible regulatory functions. They included a range of auxin-and ethylene-related transcription factors, other transcription factors and regulatory genes that are transiently induced early, 2 h after flower removal, and a set of novel AZ-specific genes. All gene expressions initiated by flower removal and leading to pedicel abscission were inhibited by indole-3-acetic acid application, while 1-methylcyclopropene pretreatment inhibited only the ethylene-induced expressions, including those induced by woundassociated ethylene signals. These results confirm our hypothesis that acquisition of ethylene sensitivity in the AZ is associated with altered expression of auxin-regulated genes resulting from auxin depletion. Our results shed light on the regulatory control of abscission at the molecular level and further expand our knowledge of auxin-ethylene cross talk during the initial controlling stages of the process.

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Overexpression of OsERF1, a novel rice ERF gene, up-regulates ethylene-responsive genes expression besides affects growth and development in Arabidopsis

Cited by 68 publications

References 29 publications

Functional validation of a drought-responsive AP2/ERF family transcription factor-encoding gene from rice in Arabidopsis

Functional validation of a drought-responsive AP2/ERF family transcription factor-encoding gene from rice in Arabidopsis

Light restored root growth of Arabidopsis with constitutive ethylene response

Microarray Analysis of the Abscission-Related Transcriptome in the Tomato Flower Abscission Zone in Response to Auxin Depletion

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