Overexpression of OgPAE1 from wild rice confers fungal resistance against Botrytis cinerea

Jeon, Eun Jeong; Chung, Eun‐Sung; Pak, Jung Hun; Nam, Jaesung; Cho, Sung Ki; Shin, Sang Hyun; Kim, Doh Hoon; Kim, Gyung Tae; Lee, Jai Heon; Kang, Kyung Ho; Chung, Young Soo

doi:10.1007/s10265-008-0164-x

Cited by 6 publications

(4 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The expression of the α 5 subunit from Oryza grandiglumis (OgPAE1) is induced during defence responses, and the overexpression of OgPAE1 in A. thaliana leads to resistance against Bo. cinerea , but the underlying mechanism(s) remains unknown (Jeon et al. , 2008).…”

Section: The Ubiquitin/proteasome Pathway In Plant Defence Reactionsmentioning

confidence: 99%

“…The expression of the a5 subunit from Oryza grandiglumis (OgPAE1) is induced during defence responses, and the overexpression of OgPAE1 in A. thaliana leads to resistance against Bo. cinerea, but the underlying mechanism(s) remains unknown (Jeon et al, 2008). The implication of the proteasome a5 subunit in plant defence might be more important in resistance to viruses, as it has been shown to specifically cleave in vitro TMV RNA Petit et al, 1997).…”

Section: Involvement Of the 26s Proteasome In Plant Defencementioning

confidence: 99%

See 1 more Smart Citation

The ubiquitin/26S proteasome system in plant–pathogen interactions: a never‐ending hide‐and‐seek game

Dielen

Badaoui

Candresse

et al. 2010

Molecular Plant Pathology

120

116

View full text Add to dashboard Cite

The ubiquitin/26S proteasome system (UPS) plays a central role in plant protein degradation. Over the past few years, the importance of this pathway in plant-pathogen interactions has been increasingly highlighted. UPS is involved in almost every step of the defence mechanisms in plants, regardless of the type of pathogen. In addition to its proteolytic activities, UPS, through its 20S RNase activity, may be part of a still unknown antiviral defence pathway. Strikingly, UPS is not only a weapon used by plants to defend themselves, but also a target for some pathogens that have evolved mechanisms to inhibit and/or use this system for their own purposes. This article attempts to summarize the current knowledge on UPS involvement in plant-microbe interactions, a complex scheme that illustrates the never-ending arms race between hosts and microbes.

show abstract

Section: The Ubiquitin/proteasome Pathway In Plant Defence Reactionsmentioning

confidence: 99%

Section: Involvement Of the 26s Proteasome In Plant Defencementioning

confidence: 99%

The ubiquitin/26S proteasome system in plant–pathogen interactions: a never‐ending hide‐and‐seek game

Dielen

Badaoui

Candresse

et al. 2010

Molecular Plant Pathology

120

116

View full text Add to dashboard Cite

show abstract

“…Fully expanded wild rice leaves were cut in approximately 2-cm-long segments, and floated on solution in sterile petri dishes. The tissues were treated with either distilled water for the control or 20% yeast extracts, wounding or various chemicals; 100 lM CN (Cantharidin), 100 lM EN (endothall), 1 mM ET (ethephon), 100 lM JA (jasmonic acid) or 100 lM SA (salicylic acid) (Jeon et al 2008;Rakwal et al 2001). Treated samples were harvested at the indicated times, and used immediately for RNA isolation or stored at -80°C.…”

Section: Plant Materials and Treatmentsmentioning

confidence: 99%

Enhanced fungal resistance in Arabidopsis expressing wild rice PR-3 (OgChitIVa) encoding chitinase class IV

Pak

Chung

Shin

et al. 2009

Plant Biotechnol Rep

View full text Add to dashboard Cite

Oryza grandiglumis Chitinase IVa (OgChitIVa) cDNA encoding a class IV chitinase was cloned from wild rice (Oryza grandiglumis). OgChitIVa cDNA contains an open reading frame of 867 nucleotides encoding 288 amino acid residues with a predicted molecular weight of 30.4 kDa and isoelectric point of 8.48. Deduced amino acid sequences of OgChitIVa include the signal peptide and chitin-binding domain in the N-terminal domain and conserved catalytic domain. OgChitIVa showed significant similarity at the amino acid level with related monocotyledonous rice and maize chitinase, but low similarity with dicotyledoneous chitinase. Southern blot analysis showed that OgChitIVa genes are present as two copies in the wild rice genome. It was shown that RNA expression of OgChitIVa was induced by defense/stress signaling chemicals, such as jasmonic acid, salicylic acid, and ethephon or cantharidin and endothall or wounding, and yeast extract. It was demonstrated that overexpression of OgChitIVa in Arabidopsis resulted in mild resistance against the fungal pathogen, Botrytis cinerea, by lowering disease rate and necrosis size. RT-PCR analysis showed that PR-1 and PR-2 RNA expression was induced in the transgenic lines. Here, we suggest that a novel OgChitIVa gene may play a role in signal transduction process in defense response against B. cinerea in plants.

show abstract

“…This species has large panicles and has potentially useful genes for many agronomic traits such as higher biomass and resistance to diseases and insect (Heinrichs et al 1985;Yu et al 1997;Vaughan et al 2003). A few studies have been conducted to detect genetic variability of O. grandiglumis (Shin et al 2004;Kim et al 2005;Jeon et al 2008Jeon et al , 2009Pak et al 2009;Jeon et al 2012;Shin et al 2012). However, attempts to transfer genes from O. grandiglumis to cultivars have been limited mainly due to incompatibility between unrelated genomes (Yu et al 1997).…”

Section: O Grandiglumis (Allotetraploid Ccdd) Is Endemic Tomentioning

confidence: 99%

Development of Near-isogenic Transgenic Rice Lines Harboring Wild Rice (Oryza grandiglumis)-Derived Fungal Resistance Gene (OgPR1)

Pak¹,

Oh²,

Kim³

et al. 2013

Plant Breed. Biotech.

Self Cite

View full text Add to dashboard Cite

This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. ABSTRACT A total of 34 T1 transgenic rice lines overexpressing OgPR1 from wild rice (Oryza grandiglumis) were produced in the previous study. Selection of transgenic plants using hygromycin selection medium was continuously done until T4 generation to find ten homozygous lines. These ten T4 lines were established in the Genetically Modified Organism (GMO) field of NICS (National Institute of Crop Science) in the year of 2007. Phenotypic uniformity and performance was evaluated and compared to the control Dongjin. Two lines were selected for Preliminary Yield Trial (PYT) and Replicated Yield Trial (RYT). Based on morpho-agronomic trait performance, the transgenic plants tended to head later than the control. Culm length was similar to the wild-type Dongjin but the panicle length was relatively reduced. In case of panicle number, transgenic lines showed a little increment compared to wild-type. The shape of grain was nearly the same to wild-type. Yield among lines showed a little difference but was statistically not significant. In terms of physiochemical property of the grain, the transgenic lines showed higher amylose content than the wild-type. When OgPR1-expressing transgenic rice plants were tested against rice blast disease, an enhanced resistance against the disease was observed in the transgenic plants.

show abstract

Overexpression of OgPAE1 from wild rice confers fungal resistance against Botrytis cinerea

Cited by 6 publications

References 33 publications

The ubiquitin/26S proteasome system in plant–pathogen interactions: a never‐ending hide‐and‐seek game

The ubiquitin/26S proteasome system in plant–pathogen interactions: a never‐ending hide‐and‐seek game

Enhanced fungal resistance in Arabidopsis expressing wild rice PR-3 (OgChitIVa) encoding chitinase class IV

Development of Near-isogenic Transgenic Rice Lines Harboring Wild Rice (Oryza grandiglumis)-Derived Fungal Resistance Gene (OgPR1)

Contact Info

Product

Resources

About