Enhanced fungal resistance in Arabidopsis expressing wild rice PR-3 (OgChitIVa) encoding chitinase class IV

Abstract

Oryza grandiglumis Chitinase IVa (OgChitIVa) cDNA encoding a class IV chitinase was cloned from wild rice (Oryza grandiglumis). OgChitIVa cDNA contains an open reading frame of 867 nucleotides encoding 288 amino acid residues with a predicted molecular weight of 30.4 kDa and isoelectric point of 8.48. Deduced amino acid sequences of OgChitIVa include the signal peptide and chitin-binding domain in the N-terminal domain and conserved catalytic domain. OgChitIVa showed significant similarity at the amino acid level with related monocotyledonous rice and maize chitinase, but low similarity with dicotyledoneous chitinase. Southern blot analysis showed that OgChitIVa genes are present as two copies in the wild rice genome. It was shown that RNA expression of OgChitIVa was induced by defense/stress signaling chemicals, such as jasmonic acid, salicylic acid, and ethephon or cantharidin and endothall or wounding, and yeast extract. It was demonstrated that overexpression of OgChitIVa in Arabidopsis resulted in mild resistance against the fungal pathogen, Botrytis cinerea, by lowering disease rate and necrosis size. RT-PCR analysis showed that PR-1 and PR-2 RNA expression was induced in the transgenic lines. Here, we suggest that a novel OgChitIVa gene may play a role in signal transduction process in defense response against B. cinerea in plants.

Cit1,2RhaT and two novel CitdGlcTs participate in flavor-related flavonoid metabolism during citrus fruit development

“…The coding sequences of Cit1,2RhaT , CitdGlcT-1 , and CitdGlcT-2 were cloned into the binary vector PH7WG2D under the control of the CAMV-35S promoter and translationally fused to the green fluorescent protein (GFP) reporter gene, via the BP and LR reaction of the Gateway system (Pak et al , 2009; Kuma et al , 2015; Rohani et al , 2016). The recombinant vector was then transferred into Agrobacterium tumefaciens GV3101 using a heat-shock method.…”

Development of Near-isogenic Transgenic Rice Lines Harboring Wild Rice (Oryza grandiglumis)-Derived Fungal Resistance Gene (OgPR1)

“…This species has large panicles and has potentially useful genes for many agronomic traits such as higher biomass and resistance to diseases and insect (Heinrichs et al 1985;Yu et al 1997;Vaughan et al 2003). A few studies have been conducted to detect genetic variability of O. grandiglumis (Shin et al 2004;Kim et al 2005;Jeon et al 2008Jeon et al , 2009Pak et al 2009;Jeon et al 2012;Shin et al 2012). However, attempts to transfer genes from O. grandiglumis to cultivars have been limited mainly due to incompatibility between unrelated genomes (Yu et al 1997).…”