Overexpression of MpCYS4 , a phytocystatin gene from Malus prunifolia (Willd.) Borkh., delays natural and stress-induced leaf senescence in apple

Tan, Yanxiao; Yang, Yingli; Li, Chao; Liang, Bowen; Li, Mingjun; Ma, Fengwang

doi:10.1016/j.plaphy.2017.03.025

Cited by 17 publications

(8 citation statements)

References 36 publications

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“…We used the M26 semi‐dwarfing rootstock for these studies. Compared with the other M series of dwarfing rootstock, M26 is a semi‐dwarfing rootstock that has a higher rooting ability, but is taller than its fully dwarfing M9 parent (Zhu & Welander, ; Tan et al ., ). M26 induces scion dwarfing; therefore, the mechanisms determining self‐dwarfing and dwarfing due to the rootstock–scion interaction can be differentiated.…”

Section: Discussionmentioning

confidence: 97%

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MdWRKY9 overexpression confers intensive dwarfing in the M26 rootstock of apple by directly inhibiting brassinosteroid synthetase MdDWF4 expression

et al. 2017

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Dwarfing rootstocks enable high-density planting and are therefore highly desirable in modern apple (Malus domestica) production. M26 is a semi-dwarfing rootstock that is used worldwide, but identifying intensive dwarfing rootstock is a major goal of apple breeding programs. Herein, we show that MdWRKY9 mediates dwarfing by directly inhibiting the transcription of the brassinosteroid (BR) rate-limiting synthetase MdDWF4 and reducing BR production. We found that the transcriptional factor MdWRKY9 is highly expressed in all tested dwarfing rootstocks. Transgenic lines of M26 rootstock overexpressing MdWRKY9 exhibit further dwarfing, which resulted from the reduced BR levels and was reversed via exogenous brassinolide treatment. Both an in vivo chromatin immunoprecipitation (ChIP) analysis and an in vitro electrophoretic mobility shift assay (EMSA) indicated that MdWRKY9 binds to the promoter of MdDWF4. Furthermore, MdWRKY9 repressed MdDWF4 expression in stable transgenic apple plants as determined by quantitative PCR. In addition, RNA-interfered expression of MdWRKY9 in transiently transformed apple calli led to a significant increase of MdDWF4, suggesting MdWRKY9 plays a critical role in regulating the expression of MdDWF4. We report a novel dwarfing mechanism in perennial woody plants that involves WRKY-controlled BR production, and present a new dwarfing M26 rootstock for potential applications in apple production.

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Section: Discussionmentioning

confidence: 97%

“…New Phytologist Welander, 1999;Tan et al, 2017). M26 induces scion dwarfing; therefore, the mechanisms determining self-dwarfing and dwarfing due to the rootstock-scion interaction can be differentiated.…”

Section: Researchmentioning

confidence: 99%

MdWRKY9 overexpression confers intensive dwarfing in the M26 rootstock of apple by directly inhibiting brassinosteroid synthetase MdDWF4 expression

et al. 2017

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“…In this case, plants often regulate the gene expression or protein activity of PLCPs through some regulators to promote plant growth and increase crop yield. The currently deep-study regulatory factors of PLCP are phytocystatins, and many researches have revealed that the overexpression of phytocystatins significantly delays plant leaf senescence and increases stress tolerance, and the direct inhibition of protease activity may be the main reason (Kunert et al, 2015; Subburaj et al, 2017; Tan et al, 2017a,b). In conclusion, the differential effects of PLCPs in different species are determined by various factors such as the spatiotemporal pattern of protease expression and action, action substrate, and specific growth period and growth state in which the plant is located.…”

Section: Plcps Mediate Plant Abiotic Stress Responsementioning

confidence: 99%

Role of Papain-Like Cysteine Proteases in Plant Development

et al. 2018

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Papain-like cysteine proteases (PLCP) are prominent peptidases found in most living organisms. In plants, PLCPs was divided into nine subgroups based on functional and structural characterization. They are key enzymes in protein proteolysis and involved in numerous physiological processes. In this paper, we reviewed the updated achievements of physiological roles of plant PLCPs in germination, development, senescence, immunity, and stress responses.

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“…Very recently, Tan, Li, Yang, Sun, et al () reported that the overexpression of the PhyCys MpCYS4 gene from Malus prunifolia enhanced stomatal closing, up‐regulated the transcriptional levels of abscisic‐acid‐ and drought‐related genes, and conferred drought tolerance in Arabidopsis and apple ( Malus domestica ) transgenic plants. Tan, Li, Yang, Li, et al () also observed delayed leaf senescence in apple MpCYS4‐overexpressing plants, in both the natural and stress‐induced scenarios. Most of the effects described are a consequence of the direct inhibition of protease targets.…”

Section: Introductionmentioning

confidence: 84%

Silencing barley cystatins HvCPI‐2 and HvCPI‐4 specifically modifies leaf responses to drought stress

Velasco-Arroyo

Díaz-Mendoza

Gomez-Sanchez

et al. 2018

Plant Cell & Environment

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Protein breakdown and mobilization are some of the major metabolic features associated with abiotic stresses, essential for nutrient recycling and plant survival. Genetic manipulation of protease and/or protease inhibitors may contribute to modulate proteolytic processes and plant responses. The expression analysis of the whole cystatin family, inhibitors of C1A cysteine proteases, after water deprivation in barley leaves highlighted the involvement of Icy-2 and Icy-4 cystatin genes. Artificial microRNA lines independently silencing the two drought-induced cystatins were generated to assess their function in planta. Phenotype alterations at the final stages of the plant life cycle are represented by the stay-green phenotype of silenced cystatin 2 lines. Besides, the enhanced tolerance to drought and differential responses to water deprivation at the initial growing stages are observed. The mutual compensating expression of Icy-2 and Icy-4 genes in the silencing lines pointed to their cooperative role. Proteolytic patterns by silencing these cystatins were concomitant with modifications in the expression of potential target proteases, in particular, HvPap-1, HvPap-12, and HvPap-16 C1A proteases. Metabolomics analysis lines also revealed specific modifications in the accumulation of several metabolites. These findings support the use of plants with altered proteolytic regulation in crop improvement in the face of climate change.

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Overexpression of MpCYS4 , a phytocystatin gene from Malus prunifolia (Willd.) Borkh., delays natural and stress-induced leaf senescence in apple

Cited by 17 publications

References 36 publications

MdWRKY9 overexpression confers intensive dwarfing in the M26 rootstock of apple by directly inhibiting brassinosteroid synthetase MdDWF4 expression

MdWRKY9 overexpression confers intensive dwarfing in the M26 rootstock of apple by directly inhibiting brassinosteroid synthetase MdDWF4 expression

Role of Papain-Like Cysteine Proteases in Plant Development

Silencing barley cystatins HvCPI‐2 and HvCPI‐4 specifically modifies leaf responses to drought stress

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