Overexpression of miR319a Affects the Balance Between Mitosis and Endoreduplication in Arabidopsis Leaves

Wang, Fulin; Zheng, Tao; Guan-ting, WU; Lang, Chunxiu; Hu, Zhanghua; Shi, Jianxin; Jin, Wei; Chen, Jinqing; Liu, Renhu

doi:10.1007/s11105-015-0893-7

Cited by 2 publications

(1 citation statement)

References 56 publications

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“…In leaf development, the overexpression of miR319 or suppression of its target TCP genes contributed to directly regulate the progression of the cell cycle genes ICK1 / KRP1 to control the G 2 -M phase of the cell cycle [ 30 ], causing an uneven leaf shape and curvature and resulting in serrated leaves in Arabidopsis [ 28 ]. The miR319-TCP module also affects the balance between mitosis and endoreduplication [ 35 ]. In the response to various stresses, overexpressing shamiR319d enhanced temperature tolerance by inhibiting the expression of GAMYB-like1 and further altering temperature and reactive oxygen species’ [ 36 ] signal transduction in tomato [ 37 ].…”

Section: Introductionmentioning

confidence: 99%

Blocking IbmiR319a Impacts Plant Architecture and Reduces Drought Tolerance in Sweet Potato

Ren

Zhang

et al. 2022

Genes

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MicroRNA319 (miR319) plays a key role in plant growth, development, and multiple resistance by repressing the expression of targeted TEOSINTE BRANCHED/CYCLOIDEA/PCF (TCP) genes. Two members, IbmiR319a and IbmiR319c, were discovered in the miR319 gene family in sweet potato (Ipomoea batatas [L.] Lam). Here, we focused on the biological function and potential molecular mechanism of the response of IbmiR319a to drought stress in sweet potato. Blocking IbmiR319a in transgenic sweet potato (MIM319) resulted in a slim and tender phenotype and greater sensitivity to drought stress. Microscopic observations revealed that blocking IbmiR319a decreased the cell width and increased the stomatal distribution in the adaxial leaf epidermis, and also increased the intercellular space in the leaf and petiole. We also found that the lignin content was reduced, which led to increased brittleness in MIM319. Quantitative real-time PCR showed that the expression levels of key genes in the lignin biosynthesis pathway were much lower in the MIM319 lines than in the wild type. Ectopic expression of IbmiR319a-targeted genes IbTCP11 and IbTCP17 in Arabidopsis resulted in similar phenotypes to MIM319. We also showed that the expression of IbTCP11 and IbTCP17 was largely induced by drought stress. Transcriptome analysis indicated that cell growth-related pathways, such as plant hormonal signaling, were significantly downregulated with the blocking of IbmiR319a. Taken together, our findings suggest that IbmiR319a affects plant architecture by targeting IbTCP11/17 to control the response to drought stress in sweet potato.

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