Overexpression of miR319a Altered Oil Body Morphogenesis and Lipid Content in Arabidopsis Seeds

Wang, Fulin; Zheng, Tao; Hu, Zhanghua; Guan-ting, WU; Lang, Chunxiu; Liu, Renhu

doi:10.1007/s11105-020-01217-y

Cited by 5 publications

(2 citation statements)

References 29 publications

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“…Oil bodies in seeds typically range between 0.5 to 2.5 µm in average diameter, with PL content of 2.5 to 0.5 mass % respectively. Data J o u r n a l P r e -p r o o f are not available for Camelina sativa, but various Brassica species have oil bodies reported in the 0.65-0.75 µm range, equivalent to 2-1.6 wt % PL (41) while Arabidopsis thaliana (Col-0) has oil bodies of 1.7 µm diameter, equivalent to 0.8 wt % PL (42). Since glyceryl labeling indicates PL accumulation at 12 mole % (Table 1), this is likely to be many fold over that required for oil body assembly.…”

Section: Biological Implications For Lipid Rate and Pool Size Determinations And Two Tag Synthesis Systemsmentioning

confidence: 99%

Kinetic complexities of triacylglycerol accumulation in developing embryos from Camelina sativa provide evidence for multiple biosynthetic systems

Pollard

Shachar‐Hill

2022

Journal of Biological Chemistry

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Quantitative flux maps describing glycerolipid synthesis can be important tools for rational engineering of lipid content and composition in oilseeds. Lipid accumulation in cultured embryos of Camelina sativa is known to mimic that of seeds in terms of rate of lipid synthesis and composition. To assess the kinetic complexity of the glycerolipid flux network, cultured embryos were incubated with [ 14 C/ 13 C]glycerol, and initial and steady state rates of [ 14 C/ 13 C glyceryl ] lipid accumulation were measured. At steady state, the linear accumulations of labeled lipid classes matched those expected from mass compositions. The system showed an apparently simple kinetic precursor–product relationship between the intermediate pool, dominated by diacylglycerol (DAG) and phosphatidylcholine (PC), and the triacylglycerol (TAG) product. We also conducted isotopomer analyses on hydrogenated lipid class species. [ 13 C 3 glyceryl ] labeling of DAG and PC, together with estimates of endogenous [ 12 C 3 glyceryl ] dilution, showed that each biosynthetically active lipid pool is ∼30% of the total by moles. This validates the concept that lipid sub-pools can describe lipid biosynthetic networks. By tracking the kinetics of [ 13 C 3 glyceryl ] and [ 13 C 2 acyl ] labeling, we observed two distinct TAG synthesis components. The major TAG synthesis flux (∼75%) was associated with >95% of the DAG/PC intermediate pool, with little glycerol being metabolized to fatty acids, and with little dilution from endogenous glycerol; a smaller flux exhibited converse characteristics. This kinetic heterogeneity was further explored using postlabeling embryo dissection and differential lipid extractions. The minor flux was tentatively localized to surface cells across the whole embryo. Such heterogeneity must be recognized in order to construct accurate gene expression patterns and metabolic networks describing lipid biosynthesis in developing embryos.

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Section: Biological Implications For Lipid Rate and Pool Size Determinations And Two Tag Synthesis Systemsmentioning

confidence: 99%

Kinetic complexities of triacylglycerol accumulation in developing embryos from Camelina sativa provide evidence for multiple biosynthetic systems

Pollard

Shachar‐Hill

2022

Journal of Biological Chemistry

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“…The overexpression of the yeast gene encoding a cytoplasmic glycerol-3-phosphate dehydrogenase (gpd1) in B. napus can lead to a 3-to 4-fold increase in the glycerol-3phosphate level and a 40% increase in the seed lipid content (Vigeolas et al, 2007). The overexpression of miR319a alters the seed oil composition of transgenic Arabidopsis, resulting in a 5.2% increase in the linoleic acid content and a 24.7% decrease in the oleic acid content (Wang et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Genetic basis of the oil biosynthesis in ultra-high-oil maize grains with an oil content exceeding 20%

Luo

Shi

et al. 2023

Front. Plant Sci.

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Vegetable oil is an important part of the human diet and has multiple industrial uses. The rapid increase in vegetable oil consumption has necessitated the development of viable methods for optimizing the oil content of plants. The key genes regulating the biosynthesis of maize grain oil remain mostly uncharacterized. In this study, by analyzing oil contents and performing bulked segregant RNA sequencing and mapping analyses, we determined that su1 and sh2-R mediate the shrinkage of ultra-high-oil maize grains and contribute to the increase in the grain oil content. Functional kompetitive allele-specific PCR (KASP) markers developed for su1 and sh2-R detected su1su1Sh2Sh2, Su1Su1sh2sh2, and su1su1sh2sh2 mutants among 183 sweet maize inbred lines. An RNA sequencing (RNA-seq) analysis indicated that genes differentially expressed between two conventional sweet maize lines and two ultra-high-oil maize lines were significantly associated with linoleic acid metabolism, cyanoamino acid metabolism, glutathione metabolism, alanine, aspartate, and glutamate metabolism, and nitrogen metabolism. A bulk segregant analysis and sequencing (BSA-seq) analysis identified another 88 genomic intervals related to grain oil content, 16 of which overlapped previously reported maize grain oil-related QTLs. The combined analysis of BSA-seq and RNA-seq data enabled the identification of candidate genes. The KASP markers for GRMZM2G176998 (putative WD40-like beta propeller repeat family protein), GRMZM2G021339 (homeobox-transcription factor 115), and GRMZM2G167438 (3-ketoacyl-CoA synthase) were significantly related to maize grain oil content. Another candidate gene, GRMZM2G099802 (GDSL-like lipase/acylhydrolase), catalyzes the final step of the triacylglycerol synthesis pathway and was expressed at significantly higher levels in the two ultra-high-oil maize lines than in the two conventional sweet maize lines. These novel findings will help clarify the genetic basis of the increased oil production in ultra-high-oil maize lines with grain oil contents exceeding 20%. The KASP markers developed in this study may be useful for breeding new high-oil sweet maize varieties.

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The Processing and Regulation of Intronic miRNAs Are Independent of Their Host Genes in Arabidopsis

Guo

Wang

et al. 2021

Plant Mol Biol Rep

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Overexpression of miR319a Altered Oil Body Morphogenesis and Lipid Content in Arabidopsis Seeds

Cited by 5 publications

References 29 publications

Kinetic complexities of triacylglycerol accumulation in developing embryos from Camelina sativa provide evidence for multiple biosynthetic systems

Kinetic complexities of triacylglycerol accumulation in developing embryos from Camelina sativa provide evidence for multiple biosynthetic systems

Genetic basis of the oil biosynthesis in ultra-high-oil maize grains with an oil content exceeding 20%

The Processing and Regulation of Intronic miRNAs Are Independent of Their Host Genes in Arabidopsis

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