Overexpression of LYRM1 induces mitochondrial impairment in 3T3-L1 adipocytes

Cao, Xiangyu; Kou, Chun-Zhao; Zhao, Yaping; Gao, Chunlin; Zhu, Chun; Zhang, Chunmei; Ji, Chenbo; Qin, Da-Ni; Zhang, Min; Guo, Xirong

doi:10.1016/j.ymgme.2010.08.010

Cited by 18 publications

(23 citation statements)

References 26 publications

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“…Not only can it influence glucose uptake and transport, but it can also have an effect on mitochondrial function (Cao et al, 2010;Kou et al, 2011). On the basis of the results in this study, it can be inferred that the mutations of LYRM1 have effects on BMTs and meat quality traits in Chinese native indigenous cattle.…”

Section: Discussionmentioning

confidence: 65%

“…Obesity itself has a high heritability and is significantly influenced by susceptibility genes and environmental factors (Walley, 2009). LYRM1 is a novel gene related to obesity, and it has effects on insulin resistance and mitochondrial dysfunction in adipocytes (Cao et al, 2010;Zhang et al, 2012), which is important to fat deposition.…”

Section: Discussionmentioning

confidence: 99%

“…The association between mitochondrial dysfunction in adipose tissue and obesity/diabetes has also been suggested (Hammarstedt et al, 2003;Dahlman et al, 2006). In addition, overexpression of LYRM1 not only can cause lower insulin-stimulated glucose uptake and a decline in intracellular ATP synthesis in 3T3-L1 adipocytes, but can also inhibit glucose transport in skeletal muscle cells through attenuated PI3K (phosphatidylinositol-3-kinase) and Akt phosphorylation (Cao et al, 2010;Kou et al, 2011). Studies have also shown that the development of insulin resistance can cause obesity (Ferrannini et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

“…Obesity is also related to many severe diseases, including type II diabetes, work disability and some kinds of cancer (Mokdad et al, 2003). LYR containing 1 (LYRM1) is a novel gene related to obesity, and is highly expressed in adipose tissue of obese patients (Cao et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

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New polymorphisms in the novel LYRM1 gene are associated with body measurement and meat quality traits in Qinchuan cattle

Li¹,

Huangpu²,

Gao³

et al. 2014

Genet. Mol. Res.

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ABSTRACT. Body measurement and meat quality traits play important roles in the evaluation of productivity in cattle; they are influenced by genetic and environmental factors. Recent studies have shown that LYRM1 is a novel gene related to obesity and may influence fat deposition. We screened for new polymorphisms in the bovine LYRM1 gene and analyzed their association with body measurement and meat quality traits in cattle. DNA samples were obtained from 572 Qinchuan cattle aged from 18 to 24 months. DNA sequencing was used to find the LYRM1 single nucleotide polymorphisms (SNPs). Sequence analysis of LYRM1 revealed four novel SNPs in exon 3: G50A in coding region, C126A, A127T, and T128A in a 3'-untranslated region. G50A, A127T and T128A showed two genotypes: AG and GG, AA and AT, AT and TT, respectively; while C126A showed three genotypes: AA, AC and CC. Analysis showed that these four polymorphisms were significantly associated with body measurement and meat quality traits in the 6927 LYMR1 gene polymorphisms in Qinchuan cattle ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 13 (3): 6926-6936 (2014) Qinchuan cattle population. We suggest that the LYRM1 gene can be used for marker-assisted selection to improve body measurement and meat quality traits in the Qinchuan cattle population.

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Section: Discussionmentioning

confidence: 65%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

New polymorphisms in the novel LYRM1 gene are associated with body measurement and meat quality traits in Qinchuan cattle

Li¹,

Huangpu²,

Gao³

et al. 2014

Genet. Mol. Res.

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“…Previous studies have suggested that it can promote proliferation and inhibit apoptosis of preadipocytes (Qiu et al, 2009a;Zhu et al, 2010), indicating that it plays a significant role in human obesity. Overexpression of LYRM1 can induce mitochondrial impairment in 3T3-L1 adipocytes and inhibit glucose transport in rat skeletal muscles (Cao et al, 2010;Kou et al, 2011). Our previous study demonstrated that LYRM1 was related to the growth and meat quality traits of Qinchuan cattle (Li et al, 2013).…”

Section: Introductionmentioning

confidence: 94%

Efficient construction of recombinant adenovirus expression vector of the Qinchuan cattle LYRM1 gene

Li¹,

Cz²,

Yr³

et al. 2015

Genet. Mol. Res.

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ABSTRACT. In this study, we cloned the coding DNA sequence (CDS) region of Qinchuan cattle LYR motif-containing 1 (LYRM1) and constructed a recombinant adenovirus expression vector to examine the function of LYRM1 on the cellular level. Total RNA was extracted from the adipose tissue of Qinchuan cattle, cDNA was obtained by reverse transcription, and polymerase chain reaction was used to amplify the CDS region of the LYRM1 gene. The CDS-containing fragment was inserted into the shuttle vector pAdTrack-CMV to construct pAdTrack-CMV-LYRM1 vector. After linearization of pAdTrack-CMV-LYRM1 and the negative control vector pAdTrack-CMV by restriction endonuclease PmeI, the vectors were transformed into Escherichia coli BJ5183 containing pAdEasy-1 to obtain the recombinant adenovirus vector pAd-LYRM1 and pAd-CMV through homologous recombination. pAd-LYRM1 and pAd-CMV were then digested by PacI and transfected into the 293A cell line. The recombinant adenovirus Ad-LYRM1 and Ad-CMV was obtained at a concentration of 7 x 10 8 and 1.3 x 10 9 9470 Y.K. Li et al. ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (3): 9469-9477 (2015) green fluorescent units/mL, respectively. Preadipocytes derived from Qinchuan cattle were separately infected with Ad-LYRM1 and Ad-CMV. Quantitative real-time polymerase chain reaction demonstrated that the expression of LYRM1 was increased by approximate 28,000-folds after the infection with recombinant adenovirus for 48 h. In conclusion, we successfully cloned the CDS region of the Qinchuan cattle LYRM1 gene, constructed the recombinant adenovirus expression vector, and obtained the adenovirus with high titer, providing valuable materials for studying the function of LYRM1 at the cellular level.

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Effects of Lyrm1 knockdown on mitochondrial function in 3 T3-L1 murine adipocytes

Zhu

Zhang

Kou

et al. 2012

J Bioenerg Biomembr

Self Cite

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To explore the effects of Lyrm1 knockdown on the mitochondrial function of 3 T3-L1 adipocytes using small interfering RNA (siRNA). 3 T3-L1 preadipocytes were infected with either a negative control (NC) expression lentivirus or a Lyrm1-shRNA expression lentivirus and induced to differentiate. The knockdown efficiency of Lrym1-specific shRNA in 3 T3-L1 cells was evaluated by real-time PCR. The ultrastructure of the mitochondria in adipocytes was visualized using transmission electron microscopy after differentiation. The levels of mitochondrial DNA copy numbers and Ucp2 mRNA were detected by real-time quantitative PCR. The levels of ATP production was detected using a photon-counting luminometer. The mitochondrial membrane potential and ROS levels of cells were analyzed with a FACScan flow cytometer using Cell Quest software. Cells transfected with lentiviral-Lyrm1-shRNA showed a significantly reduced transcription of Lyrm1 mRNA compared with NC cells. The size and ultrastructure of mitochondria in Lyrm1 knockdown adipocytes was similar to those of the NC cells. There was no significant difference in mtDNA copy number between the two groups. The total level of ATP production, mitochondrial membrane potential and Ucp2 mRNA expression levels were dramatically increased in adipocytes transfected with Lyrm1 RNAi. Furthermore, the level of ROS was dramatically decreased in Lyrm1 knockdown adipocytes. Knockdown of the Lyrm1 gene in adipocytes resulted in dramatically increased cellular ATP production, mitochondrial membrane potentials and levels Ucp2 mRNA, while ROS levels were significantly decreased. These results imply that mitochondrial function is improved in adipocytes after the knockdown of Lyrm1.

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Overexpression of LYRM1 induces mitochondrial impairment in 3T3-L1 adipocytes

Cited by 18 publications

References 26 publications

New polymorphisms in the novel LYRM1 gene are associated with body measurement and meat quality traits in Qinchuan cattle

New polymorphisms in the novel LYRM1 gene are associated with body measurement and meat quality traits in Qinchuan cattle

Efficient construction of recombinant adenovirus expression vector of the Qinchuan cattle LYRM1 gene

Effects of Lyrm1 knockdown on mitochondrial function in 3 T3-L1 murine adipocytes

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