Overexpression of hyaluronan synthase 2 and gonadotropin receptors in cumulus cells of goats subjected to one-shot eCG/FSH hormonal treatment for ovarian stimulation

Santos, Juliana Dantas Rodrigues; Batista, Ríbrio Ivan Tavares Pereira; Magalhães, L. C.; Paula, Alexander R.; Souza, Samara Silva de; Salamone, D.; Bhat, M. H.; Teixeira, Dárcio Ítalo Alves; Freitas, V. J. F.; Melo, Luciana Magalhães

doi:10.1016/j.anireprosci.2016.03.008

Cited by 3 publications

(2 citation statements)

References 64 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…*P Ͻ 0.05 vs. shNC; **P Ͻ 0.01 vs. shNC. Significant differences between Tm treatment and without the Tm group are marked as #P Ͻ 0.05 and ##P Ͻ 0.01. accumulation and expansion toward ovulation (17,39), and Igfbp4 plays an inhibiting role in follicular maturation (34). RT-qPCR revealed that Igfbp4 mRNA expression was upregulated, whereas the expression of other genes (Has2, Ptgs2, and Ptgfr) was significantly downregulated in granulosa cells after ATF6 depletion, suggesting that ATF6 plays a negative role in the regulation of Igfbp4 and plays a positive role in the regulation of Has2, Ptgs2, and Ptgfr in normal physiological process.…”

Section: Discussionmentioning

confidence: 99%

ATF6 knockdown decreases apoptosis, arrests the S phase of the cell cycle, and increases steroid hormone production in mouse granulosa cells

Xiong

Chen

Lin

et al. 2017

American Journal of Physiology-Cell Physiology

View full text Add to dashboard Cite

Activating transcription factor 6 (ATF6), a sensor protein located in the endoplasmic reticulum (ER) membrane, is an important factor in the ER stress signaling pathway. ER stress is known to be involved in folliculogenesis, follicular growth, and ovulation; however, the physiological function of ATF6 in mouse granulosa cells remains largely unknown. The aim of this study was to assess the role of ATF6 in mouse granulosa cells with respect to apoptosis, the cell cycle, and steroid hormone production, as well as several key genes related to follicular development, via RNA interference, immunohistochemical staining, real-time quantitative PCR, Western blotting, flow cytometry, terminal deoxynucleotidyltransferase-mediated deoxy-UTP nick end labeling (TUNEL) assay, and ELISA. Immunohistochemical staining revealed that ATF6 was extensively distributed in the granulosa cells of various ovarian follicles and oocytes in adult female mice. FSH or LH treatment significantly increased ATF6 protein levels in mouse granulosa cells. In the meantime, a recombinant plasmid was used to deplete ATF6 successfully using short hairpin RNA-mediated interference technology, which was verified at both the mRNA and protein levels. Flow cytometry and TUNEL assay analysis indicated that ATF6 depletion decreased apoptosis and arrested the S phase of the cell cycle in mouse granulosa cells. Consistent with these results, ,, ,, ,, and mRNA expression decreased, whereas and mRNA expression increased. Interestingly, ATF6 knockdown obviously increased progesterone and estradiol production in mouse granulosa cells. () mRNA levels were downregulated, whereas ,, and mRNA levels were upregulated, in keeping with the changes in steroid hormones. Furthermore, ATF6 disruption remarkably increased () expression and decreased (), (), and () expression in mouse granulosa cells, which are proteins crucial for follicular development. But, after treating with tunicamycin, the levels of ,, and increased relatively, whereas expression decreased. Collectively, these results imply that ATF6, as a key player in ER stress signaling, may regulate apoptosis, the cell cycle, steroid hormone synthesis, and other modulators related to folliculogenesis in mouse granulosa cells, which may indirectly be involved in the development, ovulation, and atresia of ovarian follicles by affecting the physiological function of granulosa cells. The present study extends our understanding and provides new insights into the physiological significance of ATF6, a key signal transducer of ER stress, in ovarian granulosa cells.

show abstract

Section: Discussionmentioning

confidence: 99%

ATF6 knockdown decreases apoptosis, arrests the S phase of the cell cycle, and increases steroid hormone production in mouse granulosa cells

Xiong

Chen

Lin

et al. 2017

American Journal of Physiology-Cell Physiology

View full text Add to dashboard Cite

show abstract

“…Several protocols have been proposed and tested for small ruminants, and usually involve the administration of FSH in either multiple doses (Baldassarre et al, 1996;Berlinguer et al, 2007;Sousa et al, 2011) or as a "one-shot" regimen associated with equine chorionic gonadotropin (eCG) (Baldassarre et al, 1996;Sousa et al, 2011), while its use in a single dose without the latter was ineffective (Armstrong et al, 1994;Baldassarre et al, 1996). Due to the ease of use in practical routine, the application of 80 mg FSH in a single dose with 300 IU eCG ("one-shot") is often used in small ruminants (Baldassarre et al, 2002;Pierson et al, 2004;Sousa et al, 2011;Teixeira et al, 2011;Baldassarre, 2012;Santos et al, 2016).…”

Section: Hormonal Ovarian Stimulationmentioning

confidence: 99%

In vitro embryo production in small ruminants: what is still missing?

Souza-Fabjan,

Leal,

Monteiro

et al. 2023

Anim. Reprod.

View full text Add to dashboard Cite

In vitro embryo production (IVEP) is an extremely important tool for genetic improvement in livestock and it is the biotechnology that has grown the most recently. However, multiple ovulation followed by embryo transfer is still considered the leading biotechnology for embryo production in small ruminants. This review aimed to identify what is still missing for more efficient diffusion of IVEP in small ruminants, going through the IVEP steps and highlighting the main factors affecting the outcomes. Oocyte quality is essential for the success of IVEP and an aspect to be considered in small ruminants is their reproductive seasonality and strategies to mitigate the effect of season. The logistics for oocyte collection from live females is more complex than in cattle, and tools to simplify this collection system and/or to promote an alternative way of recovering oocytes may be an important point in this scenario. The heterogeneity of oocytes collected from growing follicles in live females or from ovaries collected from abattoirs remains a challenge, and there is a demand to standardize/homogenize the hormonal stimulatory protocols and IVM protocols for each source of oocytes. The use of sexed semen is technically possible, however the low market demand associated with the high costs of the sexing process prevents the routine use of this technique, but its higher availability is an important aspect aiming for greater dissemination of IVEP. New noninvasive approaches for embryo selection are key factors since the selection for transfer or cryopreservation is another difficulty faced among laboratories. Embryo selection is based on morphological traits, although these are not necessarily reliable in predicting pregnancy. Several issues described in this review must be considered by researchers in other to promote the diffusion of IVEP in small ruminants.

show abstract

Hypoxia-Inducible Factor 1α Affects Yak Oocyte Maturation and Early Embryonic Development by Regulating Autophagy

Ma,

Wang,

Wang

et al. 2024

Antioxidants

View full text Add to dashboard Cite

In animal assisted reproductive technology, the production of high-quality oocytes is crucial. The yak, having lived in the Qinghai-Tibet Plateau for an extended period, has reproductive cells that are regulated by hypoxia-inducible factor 1α (HIF-1α). This study aimed to investigate the impact of HIF-1α on yak oocyte maturation and early embryonic development in vitro through the regulation of autophagy. The in vitro maturation process of yak oocytes involved the addition of the HIF-1α inducer DFOM and the inhibitor LW6 to examine their effects on yak oocyte maturation, early embryonic development, cell autophagy, cytochrome P450s (CYP450s) enzyme expression, and cumulus diffusion factors. The findings revealed that DFOM significantly upregulated the expression of HIF-1α, resulting in increased the cumulus diffusion area, elevated first polar body expulsion rate of oocytes, enhanced mitochondrial and actin levels, decreased ROS production, and reduced early apoptosis levels of oocytes. Moreover, DFOM promoted the expression of autophagy-related proteins, CYP450s enzymes, and cumulus diffusion factors, thereby enhancing oocyte maturation and early embryonic development. Conversely, LW6 exhibited opposite effects. The inhibition of autophagy levels with 3-MA during DFOM treatment yielded similar outcomes. Furthermore, reducing autophagy led to increased apoptosis levels at all stages of early embryonic development, as well as a significant decrease in total cell number and ICM/TE ratio of blastocysts. Studies have shown that during the in vitro maturation of yak oocytes, HIF-1α can affect the cumulus expansion area of oocytes by regulating autophagy, the first polar body excretion rate, mitochondrial level, actin level, ROS and early apoptosis level, the CYP450s enzyme, and the expression of cumulus expansion factors, thereby improving the in vitro maturation and early embryonic development of yak oocytes. These findings offer valuable insights into the reproductive regulation mechanism of yaks in hypoxic environments and suggest potential strategies for the advancement of yak assisted reproductive technology.

show abstract

Overexpression of hyaluronan synthase 2 and gonadotropin receptors in cumulus cells of goats subjected to one-shot eCG/FSH hormonal treatment for ovarian stimulation

Cited by 3 publications

References 64 publications

ATF6 knockdown decreases apoptosis, arrests the S phase of the cell cycle, and increases steroid hormone production in mouse granulosa cells

ATF6 knockdown decreases apoptosis, arrests the S phase of the cell cycle, and increases steroid hormone production in mouse granulosa cells

In vitro embryo production in small ruminants: what is still missing?

Hypoxia-Inducible Factor 1α Affects Yak Oocyte Maturation and Early Embryonic Development by Regulating Autophagy

Contact Info

Product

Resources

About