Overexpression of homogentisate phytyltransferase (HPT) and tocopherol cyclase (TC) enhances α-tocopherol content in transgenic tobacco

Harish, M. C.; Dachinamoorthy, P.; Balamurugan, S.; Murugan, Shanmugaraj Bala; Ramalingam, Sathishkumar

doi:10.1007/s10535-012-0298-5

Cited by 14 publications

(11 citation statements)

References 28 publications

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“…The overexpression of HPT and TC may cause tocopherol accumulation during plant growth. Harish and others found that the α-tocopherol content in transgenic tobacco plants was increased by 5.4-, 4.0-, and 7.1 folds with expressions of HPT , TC , and HPT:TC , respectively, when compared to the wild type [ 24 ]. Our results showed an agreement with this rule.…”

Section: Resultsmentioning

confidence: 99%

Evaluation of Biosynthesis, Accumulation and Antioxidant Activityof Vitamin E in Sweet Corn (Zea mays L.) during Kernel Development

Xie

JiHua

et al. 2017

IJMS

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Sweet corn kernels were used in this research to study the dynamics of vitamin E, by evaluatingthe expression levels of genes involved in vitamin E synthesis, the accumulation of vitamin E, and the antioxidant activity during the different stage of kernel development. Results showed that expression levels of ZmHPT and ZmTC genes increased, whereas ZmTMT gene dramatically decreased during kernel development. The contents of all the types of vitamin E in sweet corn had a significant upward increase during kernel development, and reached the highest level at 30 days after pollination (DAP). Amongst the eight isomers of vitamin E, the content of γ-tocotrienol was the highest, and increased by 14.9 folds, followed by α-tocopherolwith an increase of 22 folds, and thecontents of isomers γ-tocopherol, α-tocotrienol, δ-tocopherol,δ-tocotrienol, and β-tocopherol were also followed during kernel development. The antioxidant activity of sweet corn during kernel development was increased, and was up to 101.8 ± 22.3 μmol of α-tocopherol equivlent/100 g in fresh weight (FW) at 30 DAP. There was a positive correlation between vitamin E contents and antioxidant activity in sweet corn during the kernel development, and a negative correlation between the expressions of ZmTMT gene and vitamin E contents. These results revealed the relations amongst the content of vitamin E isomers and the gene expression, vitamin E accumulation, and antioxidant activity. The study can provide a harvesting strategy for vitamin E bio-fortification in sweet corn.

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Section: Resultsmentioning

confidence: 99%

Evaluation of Biosynthesis, Accumulation and Antioxidant Activityof Vitamin E in Sweet Corn (Zea mays L.) during Kernel Development

Xie

JiHua

et al. 2017

IJMS

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“…Leucoanthocyanidin dioxygenase was involved in biosynthesis of proanthocyanidin and vacuole development [ 54 ]. The overexpression of homogentisate phytyltransferase, considered as a key enzyme limiting tocopherol biosynthesis in lettuce, improved the content of tocopherol, a lipid soluble compound functioning as antioxidants and involved in signal transduction and transcription regulation [ 55 ]. Cytochrome P450 (CYP450) characterized as heme-thiolate enzymes was necessary for carbon assimilation, synthesis of hormones and as a structural components of cells [ 56 ].…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic Profiling and Physiological Analysis of Haloxylon ammodendron in Response to Osmotic Stress

Gao

Lü

Zhang

et al. 2017

IJMS

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Haloxylon ammodendron, a perennial xero-halophyte, is an essential species for investigating the effects of drought on desert tree. To gain a comprehensive knowledge on the responses of H. ammodendron to drought stress, we specially performed the molecular and physiological analysis of H. ammodendron in response to −0.75 MPa osmotic stress for six and 24 h in lab condition via RNA-seq and digital gene expression (DGE). In total, 87,109 unigenes with a mean length of 680 bp and 13,486 potential simple sequence repeats (SSRs) were generated, and 3353 differentially expressed genes (DEGs) in shoots and 4564 in roots were identified under stress. These DEGs were mainly related to ion transporters, signal transduction, ROS-scavenging, photosynthesis, cell wall organization, membrane stabilization and hormones. Moreover, the physiological changes of inorganic ions and organic solute content, peroxidase (POD) activity and osmotic potential were in accordance with dynamic transcript profiles of the relevant genes. In this study, a detailed investigation of the pathways and candidate genes identified promote the research on the molecular mechanisms of abiotic stress tolerance in the xero-halophytic species. Our data provides valuable genetic resources for future improvement of forage and crop species for better adaptation to abiotic stresses.

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“…Previous studies reported that overexpression of the Arabidopsis VTE1 gene in seeds of transgenic rapeseed plants, and in leaves of Arabidopsis , transgenic tobacco and transgenic lettuce, all resulted in an increase in total tocopherol, due mainly to an increase in γ-tocopherol (Kumar et al 2005 ; Kanwischer et al 2005 ; Lee et al 2007 ; Yabuta et al 2013 ; Harish et al 2013 ). In addition, overexpressing VTE1 can enhance tolerance to the environmental stresses.…”

Section: Discussionmentioning

confidence: 99%

Molecular cloning and heterologous expression analysis of JrVTE1 gene from walnut (Juglans regia)

Wang

Leslie

et al. 2015

Mol Breeding

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Tocopherol cyclase (VTE1) plays a key role in promoting the production of γ-tocopherol and improving total tocopherol content in photosynthetic organisms. Walnut is an important source of tocopherols in the human diet, and γ-tocopherol is the major tocopherol compound in walnut kernels. In this study, a full-length cDNA of the VTE1 gene was isolated from walnut using RT-PCR and RACE, and designated as JrVTE1. The full-length cDNA of the JrVTE1 gene contained a 1353-bp open-reading frame encoding a 451-amino-acid protein with a calculated molecular weight of 49.5 kDa. The deduced JrVTE1 protein had a considerable homology with other plant VTE1s and belonged to the tocopherol cyclase family. Functional characterization of JrVTE1 by heterologous expression was carried out in E. coli BL21 (DE3) and microshoot lines of the fruit trees jujube (Zizyphus jujuba var. spinosa) and pear (Pyrus communis) cultivar ‘Old Home’. JrVTE1 in E. coli expressed as a 50 kDa protein, as expected. One or two copies of the transferred JrVTE1 gene were detected in the genomes of representative transgenic lines (from the initial transgenic plants) of jujube and pear by gel blots analysis. Over-expression of JrVTE1 in jujube and pear resulted in an accumulation of tocopherol and a shift in tocopherol composition in leaf, root and stem tissues. In the transgenic jujube, the total tocopherol content increased by 29.8 μg/g in the stems of line J3, 43.7 and 22.5 μg/g in the roots and leaves of line J1, respectively, whereas in the transgenic pear it increased by 47.3 μg/g in the leaf of line P3, and 16.7 and 10.4 μg/g in roots and stems of line P9, respectively. In the examined tissues of transgenic plants, the highest accumulation rate was the γ-tocopherol. These results indicate that JrVTE1 is one of the rate-limiting enzymes for tocopherol production and could be used to improve the tocopherol content of tree crops through genetic engineering. Electronic supplementary materialThe online version of this article (doi:10.1007/s11032-015-0414-2) contains supplementary material, which is available to authorized users.

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Overexpression of homogentisate phytyltransferase (HPT) and tocopherol cyclase (TC) enhances α-tocopherol content in transgenic tobacco

Cited by 14 publications

References 28 publications

Evaluation of Biosynthesis, Accumulation and Antioxidant Activityof Vitamin E in Sweet Corn (Zea mays L.) during Kernel Development

Evaluation of Biosynthesis, Accumulation and Antioxidant Activityof Vitamin E in Sweet Corn (Zea mays L.) during Kernel Development

Transcriptomic Profiling and Physiological Analysis of Haloxylon ammodendron in Response to Osmotic Stress

Molecular cloning and heterologous expression analysis of JrVTE1 gene from walnut (Juglans regia)

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