Transcriptomic Profiling and Physiological Analysis of Haloxylon ammodendron in Response to Osmotic Stress

Abstract

Haloxylon ammodendron, a perennial xero-halophyte, is an essential species for investigating the effects of drought on desert tree. To gain a comprehensive knowledge on the responses of H. ammodendron to drought stress, we specially performed the molecular and physiological analysis of H. ammodendron in response to −0.75 MPa osmotic stress for six and 24 h in lab condition via RNA-seq and digital gene expression (DGE). In total, 87,109 unigenes with a mean length of 680 bp and 13,486 potential simple sequence repeats (SSRs) were generated, and 3353 differentially expressed genes (DEGs) in shoots and 4564 in roots were identified under stress. These DEGs were mainly related to ion transporters, signal transduction, ROS-scavenging, photosynthesis, cell wall organization, membrane stabilization and hormones. Moreover, the physiological changes of inorganic ions and organic solute content, peroxidase (POD) activity and osmotic potential were in accordance with dynamic transcript profiles of the relevant genes. In this study, a detailed investigation of the pathways and candidate genes identified promote the research on the molecular mechanisms of abiotic stress tolerance in the xero-halophytic species. Our data provides valuable genetic resources for future improvement of forage and crop species for better adaptation to abiotic stresses.

High levels of glucose alter Physcomitrella patens metabolism and trigger a differential proteomic response

“…Photosynthetic pigments such as chlorophyll a and b decreased during glucose and sorbitol exposure (Fig 7B), suggesting that in P. patens these parameters are more sensitive to high glucose than the maximal rate of PSII, also indicating that the moss is not under optimal operating conditions. In contrast to vascular plants [109][110][111][112], P. patens seems to be less sensitive to osmotic-and glucose-induced photosynthetic inhibition. The biological significance of these differences in photosynthetic activities between vascular and non-vascular plants in response to glucose deserves deeper research.…”

“…patens these parameters are more sensitive to high glucose than the maximal rate of PSII, also indicating that the moss is not under optimal operating conditions. In contrast to vascular plants [ 109 – 112 ], P . patens seems to be less sensitive to osmotic- and glucose-induced photosynthetic inhibition.…”

1H-NMR metabolomics reveals a multitarget action of Crithmum maritimum ethyl acetate extract in inhibiting hepatocellular carcinoma cell growth

“…Recent studies have underlined the role of lipid homeostasis for tumour development and progression. In a study performed in 20 patients, the level of PUFA was significantly lower compared to healthy liver tissue 47 . More recently, cholesterol was also found to be essential for cancer cell metabolism and was proposed as a promising therapeutic target 48 .…”

“Metalloestrogenic” effects of cadmium downstream of G protein-coupled estrogen receptor and mitogen-activated protein kinase pathways in human uterine fibroid cells

“…MAPK44/42 is widely expressed and is involved in the regulation of meiosis, mitosis, and postmitotic functions in many differentiated cell types. Several different stimuli, including growth factors, cytokines, and viruses can activate the MAPK44/42 pathway (Li and Zhang 2017 ; Ma et al 2010 ). Previously and in the present study, we have found that MAPK/MEK1/2 inhibitor, PD98059 can attenuate elevated phosphorylated MAPK44/42 expression levels in Cd-exposed ht-UtLM cells indicating that MAPK plays an important role in Cd’s proliferative effects.…”