Overexpression of heat shock protein gene PfHSP21.4 in Arabidopsis thaliana enhances heat tolerance

Zhang, Lu; Zhang, Qixiang; Gao, Yu; Shi, Shaochuan; Wang, Ye

doi:10.1007/s11738-014-1531-y

Cited by 18 publications

(14 citation statements)

References 47 publications

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“…It is believed that HSP plays a key part in reducing the damage caused by adversity stress [ 26 ]. In this study, the expression level of HSP was also enhanced to protect lateral branches under PBZ application, which was consistent with the reports in Primula [ 27 ].…”

Section: Discussionsupporting

confidence: 92%

Quantitative Proteomics Analysis of Herbaceous Peony in Response to Paclobutrazol Inhibition of Lateral Branching

Zhao

Gong

Hao

et al. 2015

IJMS

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Herbaceous peony (Paeonia lactiflora Pall.) is an emerging high-grade cut flower worldwide, which is usually used in wedding bouquets and known as the “wedding flower”. However, abundant lateral branches appear frequently in some excellent cultivars, and a lack of a method to remove Paeonia lactiflora lateral branches other than inefficient artificial methods is an obstacle for improving the quality of its cut flowers. In this study, paclobutrazol (PBZ) application was found to inhibit the growth of lateral branches in Paeonia lactiflora for the first time, including 96.82% decreased lateral bud number per branch, 77.79% and 42.31% decreased length and diameter of lateral branches, respectively, declined cell wall materials and changed microstructures. Subsequently, isobaric tag for relative and absolute quantitation (iTRAQ) technology was used for quantitative proteomics analysis of lateral branches under PBZ application and control. The results indicated that 178 differentially expressed proteins (DEPs) successfully obtained, 98 DEPs were up-regulated and 80 DEPs were down-regulated. Thereafter, 34 candidate DEPs associated with the inhibited growth of lateral branches were screened according to their function and classification. These PBZ-stress responsive candidate DEPs were involved in eight biological processes, which played a very important role in the growth and development of lateral branches together with the response to PBZ stress. These results provide a better understanding of the molecular theoretical basis for removing Paeonia lactiflora lateral branches using PBZ application.

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Section: Discussionsupporting

confidence: 92%

Quantitative Proteomics Analysis of Herbaceous Peony in Response to Paclobutrazol Inhibition of Lateral Branching

Zhao

Gong

Hao

et al. 2015

IJMS

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“…6d). An increase in proline content was also observed in salt-or heat-tolerant Arabidopsis with suppressed levels of proline degradation or increased expression of heat shock protein gene PfHSP21.4 (Nanjo et al 1999;Zhang et al 2014), transgenic salt-tolerant tobacco plants with enhanced levels of proline biosynthesis (Kishor et al 1995;Yang et al 2008), and transgenic salt-tolerant tomato plants overexpressing AtNHX1 (Zhang and Blumwald 2001). Therefore, it is possible that the elevated concentration of proline in transgenic plants helps to protect anti-oxidative enzymes, thus alleviating the negative effects imposed by high-temperature stress in LeHSP21 transgenic plants.…”

Section: Discussionmentioning

confidence: 90%

Constitutive Expression of a Tomato Small Heat Shock Protein Gene LeHSP21 Improves Tolerance to High-Temperature Stress by Enhancing Antioxidation Capacity in Tobacco

et al. 2015

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It is well established that small heat shock proteins (sHSPs) play an important role in thermotolerance in various organisms due to their abundance and diversity. In the present study, a chloroplast small heat shock protein gene (LeHSP21) from tomato (Lycopersicon esculentum cv PKM-1) was constitutively expressed in tobacco (Nicotiana tabacum L. cv Wisconsin 38) plants via Agrobacterium-mediated transformation. When compared to wild-type control plants, transgenic tobacco plants constitutively expressing LeHSP21, driven by the cauliflower mosaic virus 35S promoter, exhibited improved tolerance to both high temperature and oxidative stress. Furthermore, when the seedlings were subjected to high temperature treatment, the activities of anti-oxidative enzymes and the content of proline were significantly higher in transgenic plants than those in the wild-type plants. Our results presented here demonstrate the feasibility of improving high temperature and oxidative stress tolerance in plants through the expression of LeHSP21 gene.

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“…It is known that the expression of HSP genes is related to heat tolerance in plants (Wang et al 2004;Wahid et al 2007;Zhang et al 2014). GDA, which is a heat-tolerance enhancer, promotes some HSP genes in Arabidopsis.…”

Section: Expression Of Heat Shock Protein Genes By Sanguinarinementioning

confidence: 99%

“…In order to reduce the occurrence of such negative symptoms, plants accumulate compatible solutes, alter their hormone and growth regulator contents, enhance their antioxidative activities, and express heat shock proteins (HSPs) (Iba 2002;Kotak et al 2007). It is believed that the expression of HSP genes is an important factor in establishing heat tolerance in plants (Huang and Xu 2008), because positive correlations between the expression levels of HSP genes and the degree of heat tolerance have been demonstrated (Wang et al 2004;Wahid et al 2007;Waters 2013;Zhang et al 2014). Controlling the HSP expression can be an effective technique to ameliorate the heat damage sustained by plants.…”

Section: Introductionmentioning

confidence: 99%

The natural alkaloid sanguinarine promotes the expression of heat shock protein genes in Arabidopsis

Hara

Kurita

2014

Acta Physiol Plant

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Small-molecule heat shock response inducers are known to enhance heat tolerance in plants. In this paper, we report that a plant alkaloid enhances the heat tolerance of Arabidopsis. We investigated 12 commercially available alkaloids to determine whether they enhance the heat tolerance of Arabidopsis seedlings by using an in vitro assay system with geldanamycin, which is a known heat shock response inducer, as a positive control. Accordingly we found that the isoquinoline alkaloid sanguinarine can enhance heat tolerance in Arabidopsis. No such effect was shown for the other 11 alkaloids. The sanguinarine treatment increased the expression of heat shock protein genes such as HSP17.6C-CI, HSP70, and HSP90.1, which were up-regulated by geldanamycin. Treatments with other isoquinoline alkaloids (berberine and papaverine), which showed few heat-tolerance-enhancing effects, did not promote the expression of the heat shock protein genes. These results suggest that sanguinarine influenced the heat tolerance of Arabidopsis by enhancing the expression of heat shock protein genes.

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Overexpression of heat shock protein gene PfHSP21.4 in Arabidopsis thaliana enhances heat tolerance

Cited by 18 publications

References 47 publications

Quantitative Proteomics Analysis of Herbaceous Peony in Response to Paclobutrazol Inhibition of Lateral Branching

Quantitative Proteomics Analysis of Herbaceous Peony in Response to Paclobutrazol Inhibition of Lateral Branching

Constitutive Expression of a Tomato Small Heat Shock Protein Gene LeHSP21 Improves Tolerance to High-Temperature Stress by Enhancing Antioxidation Capacity in Tobacco

The natural alkaloid sanguinarine promotes the expression of heat shock protein genes in Arabidopsis

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