Overexpression of Citrus grandis DREB gene in tomato affects fruit size and accumulation of primary metabolites

Nishawy, Elsayed; Sun, Xiangming; Ewas, Mohamed; Ziaf, Khurram; Xu, Rangwei; Wang, Dan; Amar, Mohamed Hamdy; Zeng, Yunliu; Cheng, Yunjiang

doi:10.1016/j.scienta.2015.06.035

Cited by 9 publications

(6 citation statements)

References 40 publications

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“…These genes contain DRE/CRT motif in their promoter sequence (Azzeme et al 2017). Overexpression of citrus DREB in tomato, however, produced smaller fruit size and induced accumulation of primary metabolites (Nishawy et al 2015). In this study, overexpression of EgDREB1 also led to the production of smaller fruit size, but in addition, reduction in seed number and production of parthenocarpic fruit were observed.…”

Section: Introductionmentioning

confidence: 41%

“…The seed number formation influences the final size and mass of the fruit (Martinelli et al 2009). Reduction of fruit size was also observed when Citrus grandis DREB was overexpressed in tomato (Nishawy et al 2015). The use of CaMV35 promoter has been reported to increase the chances of transcriptional inactivation in certain tissues (Stam et al 1997) which could explain the non-detectable expression of EgDREB1 in roots and leaves.…”

Section: Discussionmentioning

confidence: 99%

“…The involvement of ABA in fruit development was established when the decrease in fruit size and seed numbers of tomato was observed in ABA-deficient mutants (notabilis/flacca) (Nitsch et al 2012). In fruit development, ABA is also known to be involved in seed maturation and fruit ripening (Bonghi et al 2011).…”

Section: Name Of Gene Copy Number Locationmentioning

confidence: 99%

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Overexpression of oil palm EgDREB1 in tomato decreased fruit sizeand produced parthenocarpic fruits

Azzeme¹,

Abdullah²,

Aziz³

et al. 2020

Biol. plant.

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Drought-responsive element binding (DREB) is involved in the regulation of stress-responsive gene expressions in plants through abscisic acid (ABA)-independent pathway. In this study, constitutive expression of oil palm (Elaeis guineensis) EgDREB1 driven by double strength cauliflower mosaic virus 35S promoter in tomato (Solanum lycopersicum) reduced seed number, produced parthenocarpic fruits, changed morphology of leaves, and increased root biomass of transgenic plants. Early flowering and fruiting of the transgenic lines were observed in the culture vessels. EgDREB1 was specifically expressed in the fruits and its expression was not detected in vegetative tissues (leaves and roots). Altered expression of several endogenous tomato genes involved in the biosynthesis of phytohormones including jasmonic acid, ethylene, auxin, cytokinin, gibberellin (GA) and ABA were observed compared to wild type plants. The expression of AP2-likeethylene transcription factor (LeAP2), allene oxide synthase (LeAOS), allene oxide cyclase (LeAOC), aminocyclopropane-1-carboxylic acid synthase (LeACS), 1-aminocyclopropane-1-carboxylate oxidase 1 (LeACO), auxin responsive factor 8 (LeARF8), auxin/indole-3-acetic acid (LeAux/IAA), cytokinin oxidase/dehydrogenase-like (LeSlCKX1), adenylate isopentenyltransferase (LeSlIPT1), gibberellin 2-oxidase 2 (LeGa2ox2), gibberellin 20-oxidase 4 (LeGa20ox4) and ABAaldehyde oxidase (LeAAO) were different in fruits with reduced seed number compared to parthenocarphic fruits. These results suggest that their expression has significant effects on fruit development in transgenic tomato. EgDREB1 may mediate the expression of some of these genes as dehydration-responsive element binding (DRE) motif were found in their promoter sequences. These data indicate that the EgDREB1 controls fruit development in trsngenic plants by regulating the expression of hormone-associated genes.

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Section: Introductionmentioning

confidence: 41%

Section: Discussionmentioning

confidence: 99%

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Overexpression of oil palm EgDREB1 in tomato decreased fruit sizeand produced parthenocarpic fruits

Azzeme¹,

Abdullah²,

Aziz³

et al. 2020

Biol. plant.

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“…Furthermore, Nishawy et al (2015) reported that citrus fruit size was inversely associated with the organic acid Page 15 of 19 level. In fact, they demonstrated that overexpression of the dehydration responsive element binding gene (CgDREB) led to a dramatic decrease in citrus fruit mass, in turn prompting higher organic acid accumulation.…”

Section: Qtl Detectionmentioning

confidence: 99%

Mapping of QTLs for citrus quality traits throughout the fruit maturation process on clementine (Citrus reticulata × C. sinensis) and mandarin (C. reticulata Blanco) genetic maps

Khefifi

Dumont

Costantino

et al. 2022

Tree Genetics & Genomes

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Citrus fruit quality is defined as the combination of physical and chemical traits; some of which may change during the ripening phase, e.g., acidity and sugar content. A clear understanding of their genetic control would be very helpful for marker-assisted breeding programs especially with regard to the juvenile phase and some reproductive features that hamper the selection of improved hybrids. A genetic study was thus performed on the heredity of quality traits and QTL detection based on segregation in a progeny generated from a cross between clementine cv “Commun” (Citrus × reticulata cv clementine) and mandarin cv “Willow leaf” (C. reticulata Blanco). Parental and consensus genetic linkage maps were constructed using 645 SNP and SSR markers. These maps were represented by 10 linkage groups in clementine and 12 linkage groups in mandarin, representing 75% and 58% respectively of the previously published clementine reference map. A total of 16 traits, including fruit mass, equatorial diameter, juice percentage, total soluble solids, acidity, pH, glucose, fructose, sucrose, and citric and malic acid concentrations were evaluated at three maturation dates. High variations indicating transgressive segregation were found for all traits, with normal or close to normal distributions. QTL analysis performed using the multiple QTL model allowed the detection of 34 QTLs on the three maps. QTLs were distributed in different linkage groups and generally detected at only one date of the ripening phase. The percentage of total variation explained ranged from 12 to 37% per QTL. Major QTLs (R2 ≥ 30%) were detected for equatorial diameter, glucose, and fructose (expressed in percentage dry matter) on linkage groups 8 and 9. Co-localization of QTLs controlling correlated and uncorrelated traits were mainly found on linkage groups 2, 4, 8, and 9, particularly between fruit mass and acidity.

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“…In Arabidopsis, WRKY46 functions as a transcriptional repressor of ALMT1 , regulating aluminum-induced malate secretion ( Ding et al , 2013 ). In tomato fruits, overexpression of SlAREB1 resulted in increased citric and malic acid contents, and the expression of the mitochondrial citrate synthase gene ( mCS ) was up-regulated ( Bastías et al , 2011 ), while CgDREB -overexpressing tomato fruits showed higher levels of organic acids ( Nishawy et al , 2015 ). However, transcriptional regulatory information is still very limited.…”

Section: Introductionmentioning

confidence: 99%

Citrus CitNAC62 cooperates with CitWRKY1 to participate in citric acid degradation via up-regulation of CitAco3

Wang

et al. 2017

Journal of Experimental Botany

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Overexpression of Citrus grandis DREB gene in tomato affects fruit size and accumulation of primary metabolites

Cited by 9 publications

References 40 publications

Overexpression of oil palm EgDREB1 in tomato decreased fruit sizeand produced parthenocarpic fruits

Overexpression of oil palm EgDREB1 in tomato decreased fruit sizeand produced parthenocarpic fruits

Mapping of QTLs for citrus quality traits throughout the fruit maturation process on clementine (Citrus reticulata × C. sinensis) and mandarin (C. reticulata Blanco) genetic maps

Citrus CitNAC62 cooperates with CitWRKY1 to participate in citric acid degradation via up-regulation of CitAco3

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