Citrus CitNAC62 cooperates with CitWRKY1 to participate in citric acid degradation via up-regulation of CitAco3

Li, Shaojia; X, Yin; Wang, Wenli; Liu, Xiaofen; Zhang, Bo; Chen, Kunsong

doi:10.1093/jxb/erx187

Cited by 73 publications

(55 citation statements)

References 38 publications

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“…However, NAC transcription factors may have different subcellular locations; for example. banana MaNAC1‐ 5 is located in the nucleus and MaNAC2 in the nucleus and cytoplasm (Shan et al , ), and citrus CitNAC62 is located in the cytoplasm (Li et al , ) even with nuclear localization signal (NLS). AdNAC6/7 also have NLS at their C‐terminal ends (Fig.…”

Section: Discussionmentioning

confidence: 99%

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Genome‐wide analysis of coding and non‐coding RNA reveals a conserved miR164‐NAC regulatory pathway for fruit ripening

Wang

et al. 2019

New Phytologist

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Summary Kiwifruit (Actinidia spp.) is a climacteric fruit with high sensitivity to ethylene, influenced by multiple ethylene‐responsive structural genes and transcription factors. However, the roles of other post‐transcriptional regulators (e.g. miRNAs) necessary for ripening remain elusive. High‐throughput sequencing sRNAome, degradome and transcriptome methods were used to identify further contributors to ripening control in the kiwifruit (A. deliciosa cv ‘Hayward’). Two NAM/ATAF/CUC domain transcription factors (AdNAC6 and AdNAC7), both predicted targets for miR164, showed significant upregulation by exogenous ethylene. Gene expression analysis and luciferase reporter assays indicated that Ade‐miR164 and one of its precursor miRNAs (Ade‐MIR164b) were repressed by ethylene treatment and negatively correlated with AdNAC6/7 expression. Subsequent analysis indicated that both AdNAC6 and AdNAC7 proteins are transcriptional activators and physically bind the promoters of AdACS1 (1‐aminocyclopropane‐1‐carboxylate synthase), AdACO1 (1‐aminocyclopropane‐1‐carboxylic acid oxidase), AdMAN1 (endo‐β‐mannanase) and AaTPS1 (terpene synthase). Moreover, subcellular analysis indicated that the location of the AdNAC6/7 proteins was influenced by Ade‐miR164. Multiple omics‐based approaches revealed a novel regulatory link for fruit ripening that involved ethylene‐miR164‐NAC. The regulatory pathway for miR164‐NAC is present in various fruit (e.g. Rosaceae fruit, citrus, grape), with implications for fruit ripening regulation.

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Section: Discussionmentioning

confidence: 99%

“…banana MaNAC1-5 is located in the nucleus and MaNAC2 in the nucleus and cytoplasm (Shan et al, 2012), and citrus CitNAC62 is located in the cytoplasm (Li et al, 2017a) even with nuclear localization signal (NLS). However, NAC transcription factors may have different subcellular locations; for example.…”

Section: Researchmentioning

confidence: 99%

Genome‐wide analysis of coding and non‐coding RNA reveals a conserved miR164‐NAC regulatory pathway for fruit ripening

Wang

et al. 2019

New Phytologist

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“…The DUALhunter system (Dualsystems Biotech) was used to investigate the interaction between FaERF#9 and FaMYB98. Full-length coding sequences of FaERF#9 and FaMYB98 were cloned into pDHB1 bait vector and pPR3-N prey vector, respectively, sequences were verified, and the bait plasmid was cotransformed with prey plasmid into NMY51 in the following combinations: FaMYB98-pDHB1/pPR3-N, FaMYB98-pDHB1/FaERF#9-pPR3-N, Fa-MYB98-pDHB1/pOst1-NubI, FaERF#9-pDHB1/pPR3-N, FaERF#9-pDHB1/ FaMYB98-pPR3-N, and FaERF#9-pDHB1/pOst1-NubI (Li et al, 2017a). Transformed cells were spread onto the following plates: DDO (SD medium-Trp-Leu), QDO (SD medium-Trp-Leu-His-Ade), and QDO+3-AT (QDO medium supplemented with 1 mm 3-amino-1,2,4-triazole).…”

Section: Yeast Two-hybrid Assaymentioning

confidence: 99%

An ETHYLENE RESPONSE FACTOR-MYB Transcription Complex Regulates Furaneol Biosynthesis by Activating QUINONE OXIDOREDUCTASE Expression in Strawberry

et al. 2018

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4-Hydroxy-2,5-dimethyl-3(2H)-furanone is a major contributor to the aroma of strawberry ( × ) fruit, and the last step in its biosynthesis is catalyzed by strawberry quinone oxidoreductase (FaQR). Here, an ethylene response factor (FaERF#9) was characterized as a positive regulator of the promoter. Linear regression analysis indicated that transcript levels were correlated significantly with both transcripts and furanone content in different strawberry cultivars. Transient overexpression of in strawberry fruit significantly increased expression and furaneol production. Yeast one-hybrid assays, however, indicated that FaERF#9 by itself did not bind to the promoter. An MYB transcription factor (FaMYB98) identified in yeast one-hybrid screening of the strawberry cDNA library was capable of both binding to the promoter and activating the transcription of by ∼5.6-fold. Yeast two-hybrid assay and bimolecular fluorescence complementation confirmed a direct protein-protein interaction between FaERF#9 and FaMYB98, and in combination, they activated the promoter 14-fold in transactivation assays. These results indicate that an ERF-MYB complex containing FaERF#9 and FaMYB98 activates the promoter and up-regulates 4-hydroxy-2,5-dimethyl-3(2H)-furanone biosynthesis in strawberry.

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“…Malate accumulation involves transcriptional regulation of vacuolar proton pumps and malate transporters (Xie et al, 2012; Li et al, 2017; Hu et al, 2017). It has been reported that MdbHLH3 plays a central role in the accumulation of both malate and anthocyanins by interacting with MdMYB1 and binding to the promoter of MdMYB1 (Xie et al, 2012; Hu et al, 2016a).…”

Section: Discussionmentioning

confidence: 99%

BTB-TAZ domain protein MdBT2 modulates malate accumulation by targeting a bHLH transcription factor for degradation in response to nitrate

Zhang

Cheng

et al. 2019

Preprint

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Excessive application of nitrate, an essential macronutrient and a signal regulating diverse physiological processes, decreases malate accumulation in apple fruit, but the underlying mechanism remains poorly understood. Here, we show that an apple BTB/TAZ protein MdBT2 is involved in regulating malate accumulation and vacuolar pH in response to nitrate. In vitro and in vivo assays indicate that MdBT2 interacts directly with and ubiquitinates a bHLH transcription factor, MdCIbHLH1, via the ubiquitin/26S proteasome pathway in response to nitrate. This ubiquitination results in the degradation of MdCIbHLH1 protein and reduces the transcription of MdCIbHLH1-targeted genes involved in malate accumulation and vacuolar acidification including MdVHA-A encoding a vacuolar H+-ATPase gene, and MdVHP1 encoding a vacuolar H+-pyrophosphatase gene, as well as MdALMT9 encoding a aluminum-activated malate transporter gene. A series of transgenic analyses in apple materials including fruits, plantlets and calli demonstrate that MdBT2 controls nitrate-mediated malate accumulation and vacuolar pH at least partially, if not completely, via regulating the MdCIbHLH1 protein level. Taken together, these findings reveal that MdBT2 regulates the stability of MdCIbHLH1 via ubiquitination in response to nitrate, which in succession transcriptionally reduces the expression of malate-associated genes, thereby controlling malate accumulation and vacuolar acidification in apples under high nitrate supply.

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Citrus CitNAC62 cooperates with CitWRKY1 to participate in citric acid degradation via up-regulation of CitAco3

Abstract: Two novel transcription factors, CitNAC62 and CitWRKY1, were involved in citric acid degradation in citrus fruit, potentially via enhanced expression of CitAco3.

Cited by 73 publications

References 38 publications

Genome‐wide analysis of coding and non‐coding RNA reveals a conserved miR164‐NAC regulatory pathway for fruit ripening

Genome‐wide analysis of coding and non‐coding RNA reveals a conserved miR164‐NAC regulatory pathway for fruit ripening

An ETHYLENE RESPONSE FACTOR-MYB Transcription Complex Regulates Furaneol Biosynthesis by Activating QUINONE OXIDOREDUCTASE Expression in Strawberry

BTB-TAZ domain protein MdBT2 modulates malate accumulation by targeting a bHLH transcription factor for degradation in response to nitrate

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