Overexpression of CD64 on CD14++CD16‑ and CD14++CD16+ monocytes of rheumatoid arthritis patients correlates with disease activity

Abstract

It is well-known that monocytes are a heterogeneous cell population and different monocyte subsets play important roles in rheumatoid arthritis (RA). Cluster of differentiation (CD)64 is one of Fc receptor, which initiates immunological and inflammatory reactions. However, the roles in RA remain to be elucidated. In the present study, the expression of CD64, CD40, CD163, CD206, HLA-DR, CD80 and CD86 on monocytes and the expression of CD64 on monocyte subsets were determined by flow cytometry. The expression of CD64 on monocyte subsets in patients with RA was further analyzed for their correlation with markers of autoimmune response, inflammation, disease activity of RA and serum cytokines. Compared to the health volunteers, the expression of CD64 on monocytes and each monocyte subset were significantly elevated in RA patients. The expression of CD64 on CD14CD16- and CD14++CD16+ monocytes were positively correlated with erythrocyte sedimentation rate (ESR), C-reactive protein (CRP), rheumatoid factor (RF), anti-citrullinated protein antibodies (ACPA) and disease activity score 28 (DAS28). Furthermore, the expression of CD64 on CD14++CD16+ monocytes was found to be associated with the serum level of IL-6. In conclusions, these data demonstrated the expression of CD64 on CD14++CD16- and CD14++CD16+ monocytes are elevated and associated with the disease activity in RA.

Expression of CD163 and HLA-DR molecules on the monocytes in chronic lymphocytic leukemia patients

“…It is worth mentioning that the increase in the percentage of monocytes expressing CD16 was also observed in other pathological conditions, e.g. during bacterial, viral or parasitic infections [20] and in acute or chronic conditions such as sepsis or atherosclerosis [8], chronic liver disease [21], rheumatoid arthritis [22], and renal diseases [23,24].…”

Human Bone Marrow Mesenchymal Stromal/Stem Cells Regulate the Proinflammatory Response of Monocytes and Myeloid Dendritic Cells from Patients with Rheumatoid Arthritis

“…The altered distribution of monocyte subsets described in RA patients further supports the involvement of these cells in the pathophysiology of the disease. In fact, the proportion of CD14 + CD16 + intermediate monocytes is increased in RA synovial fluid [29] and at peripheral level [27,29,33,34]; it is also correlated to disease activity [33], and predicts the response to methotrexate therapy [23,35]. In turn, the absolute number of circulating classical and intermediate monocytes is increased in treatment-refractory patients [23].…”

Monocyte subsets involved in the development of systemic lupus erythematosus and rheumatoid arthritis

“…The expression level of CD64 (FcγRI; a high affinity activating receptor) is known to be elevated in monocytes in RA patients suggesting that monocytes from RA patients are in a more differentiated and active stage compared with those from the healthy individuals (103). Moreover, the association of monocyte subsets with the development of arthritis has been examined in patients with RA.…”