Overexpression of C/<scp>EBP</scp><i>β</i> Affects The Cell Cycle Regulators and Spermatogenesis Related Genes Expression And Function of Bovine Sertoli Cells

Tang, Kai-Fu; Jin, Yunyun; Chen, F; Wang, L

doi:10.1111/rda.12724

Cited by 4 publications

(4 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Sertoli cell proliferation under diverse stimuli has been correlated with the levels of cyclins D1, D2, and E1 in different experimental models. [31][32][33][34][35][36] Moreover, p21 Cip and p27 Kip expressions have been associated with the detention of proliferation and with the increase in the levels of differentiation markers of Sertoli cells. 18,30,37 Results obtained herein show that the decreased proliferation caused by RSV treatment in 8-day-old Sertoli cells is accompanied by decreased expression of cyclins D1, D2, E1, and E2 and increased expression of p21 Cip and p27 Kip , suggesting that the observed effect of RSV on proliferation under basal conditions may be explained, at least in part, by the variation of these cell cycle regulators.…”

Section: Discussionmentioning

confidence: 99%

“…The present investigation shows that RSV reduces Sertoli cell proliferation. Sertoli cell proliferation under diverse stimuli has been correlated with the levels of cyclins D1 , D2 , and E1 in different experimental models . Moreover, p21 Cip and p27 Kip expressions have been associated with the detention of proliferation and with the increase in the levels of differentiation markers of Sertoli cells .…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Effect of resveratrol on Sertoli cell proliferation

Gorga

Rindone

Regueira

et al. 2018

J of Cellular Biochemistry

View full text Add to dashboard Cite

Resveratrol (RSV), a polyphenolic compound largely found in red grape skin, has been used as a nutritional supplement as it exhibits beneficial health effects, such as anticancer, cardioprotective, antiaging, and anti-inflammatory. Particularly, it has been shown that it participates in the mechanisms involved in cell proliferation. Sirtuin 1 (SIRT1) is considered a well-known RSV effector. Noteworthy, Sirt1-knockout animals are infertile. The aim of this study was, first, to determine whether RSV has any effect on Sertoli cell proliferation and, second, whether SIRT1, a putative target of RSV, is present in immature Sertoli cells. Sertoli cell cultures obtained from 8-day-old rats, which actively proliferate, were treated with RSV (10 and 50 µM) under basal and follicle-stimulating hormone (FSH)-stimulated conditions. Bromodeoxyuridine (BrdU) incorporation and the expression of cyclins D1, D2, D3, E1, and E2 and the Cip/Kip cell cycle inhibitors p21 and p27 were analyzed. RSV decreased BrdU incorporation and cyclins D1, D2, E1, and E2 expression and increased p21 and p27 messenger RNA (mRNA) levels. RSV also decreased FSH-stimulated BrdU incorporation and cyclins D1 and D2 mRNA levels. The effect of RSV on cMYC was also analyzed. RSV treatment did not modify basal and FSH-stimulated cMyc expression; however, it inhibited basal and FSH-stimulated cMYC transcriptional activity, suggesting a role of cMYC in RSV effects. Additionally, Sirt1 was detected in immature Sertoli cells. Altogether, these results suggest that RSV possibly, by activating SIRT1 and regulating cMYC transcriptional activity, participates in the regulation of immature Sertoli cell proliferation.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Effect of resveratrol on Sertoli cell proliferation

Gorga

Rindone

Regueira

et al. 2018

J of Cellular Biochemistry

View full text Add to dashboard Cite

show abstract

“…The differential adherent selection method was used to isolate immature bovine SCs from 6-week-old Holstein calves as described by Yang et al (2014) and Tang, Jin, Chen, and Wang (2016). The purity of a primary SCs culture was identified using the Leydig cells marker 3β-HSD and the SCs marker androgen binding protein (ABP) (Harikae, Tsunekawa, Hiramatsu, Toda, & Kurohmaru, 2012).…”

Section: Cell Isolation Culture and Identificationmentioning

confidence: 99%

GDF9 affects the development and tight junction functions of immature bovine Sertoli cells

Tang

Wang

Jin

et al. 2017

Reprod Domestic Animals

Self Cite

View full text Add to dashboard Cite

Transforming growth factor-β (TGFβ) superfamily are critical regulators of germ cell development that act as extracellular ligands of the signal transduction pathways regulating proliferation, apoptosis and other aspects of cell behaviour. As a member of the TGF-β superfamily, growth differentiation factor 9 (GDF9) plays a critical role in ovarian follicular development and the ovulation rate in females; however, its role in the testis has not been well elucidated. The aim of this study was to investigate the expression of GDF9 and its receptor genes BMPRII and ALK5 in prepuberal bovine Sertoli cells (SCs). In addition, we assessed the effects of GDF9 on immature SCs apoptosis, the cell cycle and tight junction functions. We found that GDF9 and its receptor genes BMPRII and ALK5 were expressed in immature SCs. Exogenous GDF9 significantly promoted SCs proliferation and inhibited the apoptosis of SCs by significantly upregulating Cyclin E (cell cycle) and bcl-2 (anti-apoptosis) mRNA expression and downregulating caspase-3 (pro-apoptosis) mRNA expression. Meanwhile, exogenous GDF9 significantly decreased the value of transepithelial electrical resistance by significantly downregulating claudin-11 mRNA expression and influencing the distribution of occludin. In conclusion, this study reveals that GDF9 is a key regulator of bovine SCs through the modulation of the cell cycle, apoptosis and tight junction functions.

show abstract

“…Primary Sertoli cells (PSC) were isolated from the testes of neonatal Qinchuan bulls (n = 3) and cultured as previously described (Yang et al, 2014;Tang et al, 2016). Briefly, the testes were decapsulated and digested in 1.0 mg/mL collagenase IV/DNase solution (Sigma, USA), and incubated at 37 °C in a humid environment containing 5% CO 2 until the tubules were separated (about 45 min).…”

Section: Primary Sc Isolation Culture and Identificationmentioning

confidence: 99%

Bta-miR-34b inhibits proliferation and promotes apoptosis via the MEK/ERK pathway by targeting MAP2K1 in bovine primary Sertoli cells

Zhang

Tao

et al. 2020

Journal of Animal Science

View full text Add to dashboard Cite

Immature Sertoli cell proliferation determines the final number of mature Sertoli cells and further regulates spermatogenesis. Accumulating evidence demonstrated that microRNAs (miRNA) play an important role in Sertoli cell proliferation, differentiation, and apoptosis. However, the effect and molecular mechanism of miRNA on bovine immature Sertoli cells remains to be poorly understood. In this study, miRNA sequencing of testes collected in mature (24-month-old) and immature (neonatal) bulls was conducted to determine the miRNA expression profiles. MicroRNA-34b was one of the differentially expressed miRNA and was selected for in-depth functional studies pertaining to Sertoli cell growth. The results showed that miR-34b mimic transfection in primary Sertoli cells (PSC) inhibited cell proliferation and induced cell cycle arrested at G2 phase and decreased the expression of cell cycle-related genes CCNB1, CDK1, CDC25C, and C-MYC. MicroRNA-34b overexpression also lead to increased cell apoptosis, with pro-apoptotic genes P53 and BAX upregulated, while anti-apoptotic gene BCL2 decreased. However, miR-34b knockdown had the opposite effects. Through a combination of transcriptome sequencing, bioinformatics analysis, dual luciferase reporter assay, and western blotting, mitogen-activated protein kinase kinase1 (MAP2K1), also known as MEK1, was identified as a target of miR-34b. In addition, PSC proliferation inhibition was mediated by cell cycle arrest and apoptosis with MAP2K1 interference. Overexpression of MAP2K1 effectively reversed the miR-34b-repressed PSC cell growth. Moreover, both miR-34b overexpression and MAP2K1 knock-down decreased the protein levels of P-ERK1/2, while MAP2K1 overexpression showed opposite effects. In summary, data suggest that miR-34b regulates PSC proliferation and apoptosis through MEK/ERK signaling pathway. These data provide a theoretical and experimental framework for further clarifying the regulation of cell growth in PSC of bovine.

show abstract

Overexpression of C/EBPβ Affects The Cell Cycle Regulators and Spermatogenesis Related Genes Expression And Function of Bovine Sertoli Cells

Cited by 4 publications

References 34 publications

Effect of resveratrol on Sertoli cell proliferation

Effect of resveratrol on Sertoli cell proliferation

GDF9 affects the development and tight junction functions of immature bovine Sertoli cells

Bta-miR-34b inhibits proliferation and promotes apoptosis via the MEK/ERK pathway by targeting MAP2K1 in bovine primary Sertoli cells

Contact Info

Product

Resources

About