GDF9 affects the development and tight junction functions of immature bovine Sertoli cells

Tang, Kai-Fu; Wang, L; Jin, Yaping; Yang, Liguo

doi:10.1111/rda.12960

Cited by 8 publications

(5 citation statements)

References 43 publications

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“…In rats, Gdf9 was shown to be produced in round spermatids and regulated formation of tight junctions between Sertoli cells by inhibiting expression of tight junction-associated proteins through TGF-β signaling [28]. Similar results were observed in cattle Sertoli cells in vitro; however, exogenous GDF9 was shown to promote Sertoli cell proliferation and inhibit apoptosis [29]. Moreover, Gdf9 −/− male mice were observed to be normal and fertile [4], but mutations in Gdf9 have not been investigated in males for other species.…”

Section: Discussionsupporting

confidence: 57%

Inactivation of growth differentiation factor 9 blocks folliculogenesis in pigs

Chen

Monarch

et al. 2023

Biology of Reproduction

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Growth differentiation factor 9 (GDF9) is a secreted protein belonging to the transforming growth factor (TGF)-beta superfamily and has been well characterized for its role during folliculogenesis in the ovary. Although previous studies in mice and sheep have shown that mutations in GDF9 disrupt follicular progression, the exact role of GDF9 in pigs has yet to be elucidated. The objective of this study was to understand the role of GDF9 in ovarian function by rapidly generating GDF9 knockout (GDF9−/−) pigs by using the CRISPR/Cas9 system. Three single guide RNAs designed to disrupt porcine GDF9 were injected with Cas9 mRNA into zygotes, and blastocyst-stage embryos were transferred into surrogates. One pregnancy was sacrificed on day 100 of gestation to investigate the role of GDF9 during oogenesis. Four female fetuses were recovered with one predicted to be GDF9−/− and the others with in-frame mutations. All four had fully formed oocytes within primordial follicles, confirming that knockout of GDF9 does not disrupt oogenesis. Four GDF9 mutant gilts were generated and were grown past puberty. One gilt was predicted to completely lack functional GDF9 (GDF9−/−), and the gilt never demonstrated standing estrus and had a severely underdeveloped reproductive tract with large ovarian cysts. Further examination revealed that the follicles from the GDF9−/− gilt did not progress past preantral stages, and the uterine vasculature was less extensive than the control pigs. By using the CRISPR/Cas9 system, we demonstrated that GDF9 is a critical growth factor for proper ovarian development and function in pigs.

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Section: Discussionsupporting

confidence: 57%

Inactivation of growth differentiation factor 9 blocks folliculogenesis in pigs

Chen

Monarch

et al. 2023

Biology of Reproduction

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“…GDF9 and BMP15, two members of the transforming growth factor beta family, were considered as female related genes promoting the oocyte maturation and folliculogenesis [29,30]. However, several studies have reported the expression of gdf9 and bmp15 in testis, especially in male germ cells [31][32][33] and somatic cells [34]. The significantly The merge image of (e-g).…”

Section: Discussionmentioning

confidence: 99%

Transcriptome profiling of laser-captured germ cells and functional characterization of zbtb40 during 17alpha-methyltestosterone-induced spermatogenesis in orange-spotted grouper (Epinephelus coioides)

Yang

Zhong

et al. 2020

BMC Genomics

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Background: Spermatogenesis is an intricate process regulated by a finely organized network. The orange-spotted grouper (Epinephelus coioides) is a protogynous hermaphroditic fish, but the regulatory mechanism of its spermatogenesis is not well-understood. In the present study, transcriptome sequencing of the male germ cells isolated from orange-spotted grouper was performed to explore the molecular mechanism underlying spermatogenesis. Results: In this study, the orange-spotted grouper was induced to change sex from female to male by 17alphamethyltestosterone (MT) implantation. During the spermatogenesis, male germ cells (spermatogonia, spermatocytes, spermatids, and spermatozoa) were isolated by laser capture microdissection. Transcriptomic analysis for the isolated cells was performed. A total of 244,984,338 clean reads were generated from four cDNA libraries. Real-time PCR results of 13 genes related to sex differentiation and hormone metabolism indicated that transcriptome data are reliable. RNA-seq data showed that the female-related genes and genes involved in hormone metabolism were highly expressed in spermatogonia and spermatozoa, suggesting that these genes participate in the spermatogenesis. Interestingly, the expression of zbtb family genes showed significantly changes in the RNA-seq data, and their expression patterns were further examined during spermatogenesis. The analysis of cellular localization of Eczbtb40 and the co-localization of Eczbtb40 and Eccyp17a1 in different gonadal stages suggested that Eczbtb40 might interact with Eccyp17a1 during spermatogenesis. Conclusions:Our study, for the first time, investigated the transcriptome of the male germ cells from orangespotted grouper, and identified functional genes, GO terms, and KEGG pathways involved in spermatogenesis. Furthermore, Eczbtb40 was first characterized and its role during spermatogenesis was predicted. These data will contribute to future studies on the molecular mechanism of spermatogenesis in teleosts.

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“…As an oocyte-specific marker [19,23] [29,30]. However, several studies have reported the expression of gdf9 and bmp15 in testis, especially in male germ cells [31][32][33] and somatic cells [34].…”

Section: Discussionmentioning

confidence: 99%

Transcriptome profiling of laser-captured germ cells and functional characterization of zbtb40 during 17alpha-methyltestosterone-induced spermatogenesis in orange-spotted grouper (Epinephelus coioides)

Wu¹,

Yang²,

Zhong³

et al. 2019

Preprint

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Background: Spermatogenesis is an intricate process regulated by a finely organized network. The orange-spotted grouper (Epinephelus coioides) is a protogynous hermaphroditic fish, but the regulatory mechanism of its spermatogenesis is not well-understood. In the present study, transcriptome sequencing of the male germ cells isolated from orange-spotted grouper was performed to explore the molecular mechanism underlying spermatogenesis. Results: In this study, the orange-spotted grouper was induced to change sex from female to male by 17alpha-methyltestosterone (MT) implantation. During the spermatogenesis, male germ cells (spermatogonia, spermatocytes, spermatids, and spermatozoa) were isolated by laser capture microdissection. Transcriptomic analysis for the isolated cells was performed. A total of 244,984,338 clean reads were generated from four cDNA libraries. Real-time PCR results of 13 genes related to sex differentiation and hormone metabolism indicated that transcriptome data are reliable. RNA-seq data showed that the female-related genes and genes involved in hormone metabolism were highly expressed in spermatogonia and spermatozoa, suggesting that these genes participate in the spermatogenesis. Interestingly, the expression of zbtb family genes showed significantly changes in the RNA-seq data, and their expression patterns were further examined during spermatogenesis. The analysis of cellular localization of Eczbtb40 and the co-localization of Eczbtb40 and Eccyp17a1 in different gonadal stages suggested that Eczbtb40 might interact with Eccyp17a1 during spermatogenesis. Conclusions: For the first time, our study investigated the transcriptome of the male germ cells from orange-spotted grouper, and identified functional genes, GO terms, and KEGG pathways involved in spermatogenesis. Furthermore, Eczbtb40 was first characterized and predicted the role during spermatogenesis. These data will contribute to future studies on the molecular mechanism of spermatogenesis in teleosts.

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GDF9 affects the development and tight junction functions of immature bovine Sertoli cells

Cited by 8 publications

References 43 publications

Inactivation of growth differentiation factor 9 blocks folliculogenesis in pigs

Inactivation of growth differentiation factor 9 blocks folliculogenesis in pigs

Transcriptome profiling of laser-captured germ cells and functional characterization of zbtb40 during 17alpha-methyltestosterone-induced spermatogenesis in orange-spotted grouper (Epinephelus coioides)

Transcriptome profiling of laser-captured germ cells and functional characterization of zbtb40 during 17alpha-methyltestosterone-induced spermatogenesis in orange-spotted grouper (Epinephelus coioides)

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