Overexpression of AtNAC2 (ANAC092) in groundnut (Arachis hypogaea L.) improves abiotic stress tolerance

Patil, Mahesh; Ramu, S. V.; Jathish, P.; Sreevathsa, Rohini; Reddy, P. Chandrashekar; Prasad, T. G.; Udayakumar, M.

doi:10.1007/s11816-013-0305-0

Cited by 36 publications

(20 citation statements)

References 39 publications

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“…Apart from these reports, transgenic peanut plants exhibited higher activities of enzymatic antioxidants that contributed to oxidative stress regulation (Pandurangaiah et al., ). Consistent with these reports, the over‐expression of AtNAC2 gene in peanut conferred abiotic stress tolerance (Patil et al., ).…”

Section: Biotechnological Approaches For Improving Abiotic Stress Tolsupporting

confidence: 71%

Peanut (Arachis hypogaea L.): A Prospective Legume Crop to Offer Multiple Health Benefits Under Changing Climate

Akram

Shafiq

Ashraf

2018

Comp Rev Food Sci Food Safe

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Peanut is a multipurpose oil-seed legume, which offer benefits in many ways. Apart from the peanut plant's beneficial effects on soil quality, peanut seeds are nutritious and medicinally and economically important. In this review, insights into peanut origin and its domestication are provided. Peanut is rich in bioactive components, including phenolics, flavonoids, polyphenols, and resveratrol. In addition, the involvement of peanut in biological nitrogen fixation is highly significant. Recent reports regarding peanut responses and N 2 fixation ability in response to abiotic stresses, including drought, salinity, heat stress, and iron deficiency on calcareous soils, have been incorporated. As a biotechnological note, recent advances in the development of transgenic peanut plants are also highlighted. In this context, regulation of transcriptional factors and gene transfer for the development of stress-tolerant peanut genotypes are of prime importance. Above all, this review signifies the importance of peanut cultivation and human consumption in view of the scenario of changing world climate in order to maintain food security.

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Section: Biotechnological Approaches For Improving Abiotic Stress Tolsupporting

confidence: 71%

Peanut (Arachis hypogaea L.): A Prospective Legume Crop to Offer Multiple Health Benefits Under Changing Climate

Akram

Shafiq

Ashraf

2018

Comp Rev Food Sci Food Safe

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“…The transgenic plants possessed efficient anti oxidative machinery which protected the plant cells from ROS induced oxidative damage. In other studies also increase in level of oxidative markers were reported when heterologous genes expressed in groundnut such as AtNHX (Asif et al, 2011), PHD45 (Manjulatha et al, 2014), AtNAC2 (Patil et al, 2014), MuNAC4 (Pandurangaiah et al, 2014). …”

Section: Discussionmentioning

confidence: 88%

“…The genes from Salicornia brachiata, ascorbate peroxidase ( SbAPX ), and abscisic acid stress ripening ( SbASR ) genes when expressed in groundnut displayed salt and drought stress tolerance coupled with more chlorophyll retention and enhanced levels of relative water content under normal/stress conditions (Singh et al, 2014; Tiwari et al, 2015). The transcription factors from Arabidopsis, AtNAC2 (Patil et al, 2014), AtDREB1A (Sarkar et al, 2014, 2016) and horsegram, MuNAC4 (Pandurangaiah et al, 2014) were exhibited drought and salt stress tolerance in groundnut by reducing the membrane damage and improving scavenging of reactive oxygen species scavenging (ROS). Groundnut transgenic plants ( cv TMV-2) simultaneously expressing three TFs ( AtDREB2A, AtHB7 , and AtABF3 ) displayed to increase drought, salinity, and oxidative stress tolerance compared to wild type (Pruthvi et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Expression of Pennisetum glaucum Eukaryotic Translational Initiation Factor 4A (PgeIF4A) Confers Improved Drought, Salinity, and Oxidative Stress Tolerance in Groundnut

et al. 2017

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Eukaryotic translational initiation factor 4A belong to family of helicases, involved in multifunctional activities during stress and non-stress conditions. The eIF4A gene was isolated and cloned from semi-arid cereal crop of Pennisetum glaucum. In present study, the PgeIF4A gene was expressed under the regulation of stress inducible Arabidopsis rd29A promoter in groundnut (cv JL-24) with bar as a selectable marker. The de-embryonated cotyledons were infected with Agrobacterium tumefaciens (LBA4404) carrying rd29A:PgeIF4A construct and generated high frequency of multiple shoots in phosphinothricin medium. Twenty- four T0 plants showed integration of both nos-bar and rd29A-PgeIF4A gene cassettes in genome with expected amplification products of 429 and 654 bps, respectively. Transgene copy number integration was observed in five T0 transgenic plants through Southern blot analysis. Predicted Mendelian ratio of segregation (3:1) was noted in transgenic plants at T1 generation. The T2 homozygous lines (L1-5, L8-2, and L16-2) expressing PgeIF4A gene were exhibited superior growth performance with respect to phenotypic parameters like shoot length, tap root length, and lateral root formation under simulated drought and salinity stresses compared to the wild type. In addition, the chlorophyll retention was found to be higher in these plants compared to the control plants. The quantitative real time—PCR results confirmed higher expression of PgeIF4A gene in L1-5, L8-3, and L16-2 plants imposed with drought/salt stress. Further, the salt stress tolerance was associated with increase in oxidative stress markers, such as superoxide dismutase accumulation, reactive oxygen species scavenging, and membrane stability in transgenic plants. Taken together our results confirmed that the PgeIF4A gene expressing transgenic groundnut plants exhibited better adaptation to stress conditions.

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“…Functional characterizations of the examined NAC TFs indicated that they participate in regulating diverse plant defending and adaptive activities, which are related to reactive oxygen species (ROS) removal, germination, root development, transpiration, photosynthetic performance, senescence, membrane transport and stabilization, nutrient remobilization and production of carbohydrate and osmo-protective compounds [51,54,[58][59][60][61][62][63]. Furthermore, the NAC-induced resistance coupled with minimized yield loss in transgenic plants upon stress exposure has also been reported in several studies [62,64,65]. These lines of evidence highlight the NAC TF family as an important resource of candidate genes that can be used for improvement of crop yields under water scarcity by genetic engineering.…”

Section: Introductionmentioning

confidence: 77%

The Soybean GmNAC019 Transcription Factor Mediates Drought Tolerance in Arabidopsis in an Abscisic Acid-Dependent Manner

Hoang

Nguyen

et al. 2019

IJMS

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Being master regulators of gene expression, transcription factors (TFs) play important roles in determining plant growth, development and reproduction. To date, many TFs have been shown to positively mediate plant responses to environmental stresses. In the current study, the biological functions of a stress-responsive NAC [NAM (No Apical Meristem), ATAF1/2 (Arabidopsis Transcription Activation Factor1/2), CUC2 (Cup-shaped Cotyledon2)]-TF encoding gene isolated from soybean (GmNAC019) in relation to plant drought tolerance and abscisic acid (ABA) responses were investigated. By using a heterologous transgenic system, we revealed that transgenic Arabidopsis plants constitutively expressing the GmNAC019 gene exhibited higher survival rates in a soil-drying assay, which was associated with lower water loss rate in detached leaves, lower cellular hydrogen peroxide content and stronger antioxidant defense under water-stressed conditions. Additionally, the exogenous treatment of transgenic plants with ABA showed their hypersensitivity to this phytohormone, exhibiting lower rates of seed germination and green cotyledons. Taken together, these findings demonstrated that GmNAC019 functions as a positive regulator of ABA-mediated plant response to drought, and thus, it has potential utility for improving plant tolerance through molecular biotechnology.

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Overexpression of AtNAC2 (ANAC092) in groundnut (Arachis hypogaea L.) improves abiotic stress tolerance

Cited by 36 publications

References 39 publications

Peanut (Arachis hypogaea L.): A Prospective Legume Crop to Offer Multiple Health Benefits Under Changing Climate

Peanut (Arachis hypogaea L.): A Prospective Legume Crop to Offer Multiple Health Benefits Under Changing Climate

Expression of Pennisetum glaucum Eukaryotic Translational Initiation Factor 4A (PgeIF4A) Confers Improved Drought, Salinity, and Oxidative Stress Tolerance in Groundnut

The Soybean GmNAC019 Transcription Factor Mediates Drought Tolerance in Arabidopsis in an Abscisic Acid-Dependent Manner

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