Overexpression of Arabidopsis NIMIN1 results in salicylate intolerance

Mohan, Rajinikanth; Tai, Thomson; Chen, Andy; Arnoff, Taylor E.; Fu, Zhenfang

doi:10.1080/15592324.2016.1211222

Cited by 6 publications

(3 citation statements)

References 21 publications

(28 reference statements)

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“…The molecular characterisation by qPCR of the phospho-modified lines 4 hours after flg22 treatment showed differential regulation of the SA response marker PR1 being higher expressed in IDD4-AA lines than in IDD4-DD and WT (S4B Fig). By contrast, the expression of the SA-signaling inhibitors WRKY38 [72] and NIMIN1 [73] are elevated in the IDD4-DD lines and the expression of NIMIN1 is reduced in IDD4-AA compared to WT and IDD4-DD line (S4C and S4D Fig). These expression patterns of SA-signaling markers indicate a reduced SA response in the IDD4-DD lines.…”

Section: Resultsmentioning

confidence: 99%

INDETERMINATE-DOMAIN 4 (IDD4) coordinates immune responses with plant-growth in Arabidopsis thaliana

Völz

Kim

et al. 2019

PLoS Pathog

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INDETERMINATE DOMAIN (IDD)/ BIRD proteins are a highly conserved plant-specific family of transcription factors which play multiple roles in plant development and physiology. Here, we show that mutation in IDD4/IMPERIAL EAGLE increases resistance to the hemi-biotrophic pathogen Pseudomonas syringae, indicating that IDD4 may act as a repressor of basal immune response and PAMP-triggered immunity. Furthermore, the idd4 mutant exhibits enhanced plant-growth indicating IDD4 as suppressor of growth and development. Transcriptome comparison of idd4 mutants and IDD4ox lines aligned to genome-wide IDD4 DNA-binding studies revealed major target genes related to defense and developmental-biological processes. IDD4 is a phospho-protein that interacts and becomes phosphorylated on two conserved sites by the MAP kinase MPK6. DNA-binding studies of IDD4 after flg22 treatment and with IDD4 phosphosite mutants show enhanced binding affinity to ID1 motif-containing promoters and its function as a transcriptional regulator. In contrast to the IDD4-phospho-dead mutant, the IDD4 phospho-mimicking mutant shows altered susceptibility to PstDC3000, salicylic acid levels and transcriptome reprogramming. In summary, we found that IDD4 regulates various hormonal pathways thereby coordinating growth and development with basal immunity.

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Section: Resultsmentioning

confidence: 99%

INDETERMINATE-DOMAIN 4 (IDD4) coordinates immune responses with plant-growth in Arabidopsis thaliana

Völz

Kim

et al. 2019

PLoS Pathog

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“…NIMIN1 interacts with NPR1 to negatively regulate NPR1-mediated immune responses (137). Knocking out NIMIN1 results in stronger SA-induced PR1 expression, whereas NIMIN1 overexpression causes phenotypes similar to npr1 mutants, including reduced SA-induced PR1 expression, compromised SAR, and SA intolerance in Arabidopsis (78,137). In contrast, overexpression of a NIMIN1 mutant protein that is unable to interact with NPR1 has no effect on SA responses and SAR, indicating that the suppression of plant defense by NIMIN1 requires its interaction with NPR1 (137).…”

Section: Feedback Amplification Of Salicylic Acid Biosynthesis and Si...mentioning

confidence: 99%

Salicylic Acid: Biosynthesis and Signaling

Peng

Yang

et al. 2021

Annu. Rev. Plant Biol.

282

176

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Salicylic acid (SA) is an essential plant defense hormone that promotes immunity against biotrophic and semibiotrophic pathogens. It plays crucial roles in basal defense and the amplification of local immune responses, as well as the establishment of systemic acquired resistance. During the past three decades, immense progress has been made in understanding the biosynthesis, homeostasis, perception, and functions of SA. This review summarizes the current knowledge regarding SA in plant immunity and other biological processes. We highlight recent breakthroughs that substantially advanced our understanding of how SA is biosynthesized from isochorismate, how it is perceived, and how SA receptors regulate different aspects of plant immunity. Some key questions in SA biosynthesis and signaling, such as how SA is produced via another intermediate benzoic acid and how SA affects the activities of its receptors in the transcriptional regulation of defense genes, remain to be addressed. Expected final online publication date for the Annual Review of Plant Biology, Volume 72 is May 2021. Please see http://www.annualreviews.org/page/journal/pubdates for revised estimates.

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“…However, consistent with a widely conserved response of both plant species to SA, the Arabidopsis orthologs of the here identi ed SA-responsive soybean markers (GmNIMIN1, GmNIMIN1.2, GmWRKY40, GmUGT and GMGH3) encode proteins, or belong to protein families, that are closely associated with SA signaling in Arabidopsis. In fact, all of these genes or members of their gene families are reported to inhibit SA-responses either by directly depleting levels of free SA through conjugation with glucose or amino acids (members of the UGT and GH3 families, respectively) 67-70 or, as is the case for the negative regulator of NPR1 functions NIMIN1 and also WRKY40, acting downstream of SA and eventually modulating the activation of SA-pathway associated genes [71][72][73][74] to maintain immune homeostasis thus preventing an excessive defense response. Although it is tempting to speculate that the identi ed soybean SA-marker genes have similar biochemical functions as in Arabidopsis, functional characterization is needed to con rm their presumed function in the growth-to-defense trade-off in soybean.…”

Section: Discussionmentioning

confidence: 99%

Disclosure of Salicylic Acid and Jasmonic Acid-responsive Genes Provides a Molecular Tool for Deciphering Stress Responses in Soybean

Beyer

Sánchez-Bel

Flors

et al. 2021

Preprint

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Hormones orchestrate the physiology of organisms. Measuring the activity of defense hormone-responsive genes can help understanding immune signaling and facilitate breeding for plant health. However, different from model species like Arabidopsis, genes that respond to defense hormones salicylic acid (SA) and jasmonic acid (JA) have not been disclosed in the soybean crop. We performed global transcriptome analyses to fill this knowledge gap. Upon exogenous application, endogenous levels of SA and JA increased in leaves. SA predominantly activated genes linked to systemic acquired resistance and defense signaling whereas JA mainly activated wound response-associated genes. In general, SA-responsive genes were activated earlier than those responding to JA. Consistent with the paradigm of biotrophic pathogens predominantly activating SA responses, free SA and here identified most robust SA marker genes GmNIMIN1, GmNIMIN1.2 and GmWRK40 were induced upon inoculation with Phakopsora pachyrhizi, whereas JA marker genes did not respond to infection with the biotrophic fungus. Spodoptera exigua larvae caused a strong accumulation of JA-Ile and JA-specific mRNA transcripts of GmBPI1, GmKTI1 and GmAAT whereas neither free SA nor SA-marker gene transcripts accumulated upon insect feeding. Our study provides molecular tools for monitoring the dynamic accumulation of SA and JA, e.g. in a given stress condition.

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Overexpression of Arabidopsis NIMIN1 results in salicylate intolerance

Cited by 6 publications

References 21 publications

INDETERMINATE-DOMAIN 4 (IDD4) coordinates immune responses with plant-growth in Arabidopsis thaliana

INDETERMINATE-DOMAIN 4 (IDD4) coordinates immune responses with plant-growth in Arabidopsis thaliana

Salicylic Acid: Biosynthesis and Signaling

Disclosure of Salicylic Acid and Jasmonic Acid-responsive Genes Provides a Molecular Tool for Deciphering Stress Responses in Soybean

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