Overexpression of an Arabidopsis cysteine-rich receptor-like protein kinase, CRK5, enhances abscisic acid sensitivity and confers drought tolerance

Lü, Kai; Liang, Shan; Wu, Zhiyong; Bi, Chao; Yu, Yongtao; Wang, Xiaofang; Zhang, David

doi:10.1093/jxb/erw266

Cited by 88 publications

(56 citation statements)

References 62 publications

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“…Overexpression of a membrane-localized cysteine-rich receptor-like protein kinase, CRK5 in Arabidopsis, led to increase of ABA sensitivity and promotion of stomatal closure, and subsequent enhancement of plant drought tolerance. Knockout of AtWRKY18, AtWRKY40 and AtWRKY60 significantly increased the expression of CRK5, suggesting negative regulation of these three genes on CRK5 [29]. In our study, the relative expression levels of two orthologous of AtWRKY40, HaWRKY74…”

Section: Discussionsupporting

confidence: 50%

Genome-wide characterization of WRKY gene family in Helianthus annuus L. and their expression profiles under biotic and abiotic stresses

Chong

Islam

et al. 2020

Preprint

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Background: WRKY transcription factors play important roles in various physiological processes and stress responses in flowering plants. However, the information about WRKY genes in Helianthus annuus L. (common sunflower) is limited. Results: Ninety WRKY (HaWRKY) genes were identified and renamed according to their locations on chromosomes. Further phylogenetic analyses classified them into four main groups including a species-specific WKKY group and HaWRKY genes within same group or subgroup generally showed similar exon-intron structures and motif compositions. The tandem and segmental duplication possibly contributed to the diversity and expansion of HaWRKY gene families. Synteny analyses of sunflower WRKY genes provided deep insight to the evolution of HaWRKY genes. Transcriptomic and qRT-PCR analyses of HaWRKY genes displayed distinct expression patterns in different plant tissues, as well as under various abiotic and biotic stresses. Conclusions: Ninety WRKY (HaWRKY) genes were identified from H. annuus L. and classified into four groups. Structures of HaWRKY proteins and their evolutionary characteristics were also investigated. The characterization of HaWRKY genes and their expression profiles under biotic and abiotic stresses in this study provide a foundation for further functional analyses of these genes and will be beneficial to crop improvement.

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Section: Discussionsupporting

confidence: 50%

Genome-wide characterization of WRKY gene family in Helianthus annuus L. and their expression profiles under biotic and abiotic stresses

Chong

Islam

et al. 2020

Preprint

View full text Add to dashboard Cite

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“…Overexpression of a membrane-localized cysteine-rich receptor-like protein kinase, CRK5 in Arabidopsis, led to increase of ABA sensitivity and promotion of stomatal closure, and subsequent enhancement of plant drought tolerance. Knockout of AtWRKY18, AtWRKY40 and AtWRKY60 signi cantly increased the expression of CRK5, suggesting negative regulation of these three genes on CRK5 [32]. In our study, the relative expression levels of two orthologous of AtWRKY40, HaWRKY74 and HaWRKY81, were recorded in sun ower roots and leaves.…”

Section: Discussionsupporting

confidence: 50%

Genome-wide characterization of WRKY gene family in Helianthus annuus L. and their expression profiles under biotic and abiotic stresses

Chong

Islam

et al. 2020

Preprint

View full text Add to dashboard Cite

Abstract Background: WRKY transcription factors play important roles in various physiological processes and stress responses in flowering plants. However, the information about WRKY genes in Helianthus annuus L. (common sunflower) is limited. Results: Ninety WRKY (HaWRKY) genes were identified and renamed according to their locations on chromosomes. Further phylogenetic analyses classified them into four main groups including a species-specific WKKY group and HaWRKY genes within same group or subgroup generally showed similar exon-intron structures and motif compositions. The tandem and segmental duplication possibly contributed to the diversity and expansion of HaWRKY gene families. Synteny analyses of sunflower WRKY genes provided deep insight to the evolution of HaWRKY genes. Transcriptomic and qRT-PCR analyses of HaWRKY genes displayed distinct expression patterns in different plant tissues, as well as under various abiotic and biotic stresses. Conclusions: Ninety WRKY (HaWRKY) genes were identified from H. annuus L. and classified into four groups. Structures of HaWRKY proteins and their evolutionary characteristics were also investigated. The characterization of HaWRKY genes and their expression profiles under biotic and abiotic stresses in this study provide a foundation for further functional analyses of these genes. Therefore, these functional genes related to increasing the plant tolerance or improving the crop quality, could be applied for the crop improvement..

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“…Apoplastic H 2 O 2 sensing may be achieved by cysteine‐rich RLKs (CRKs) localized on the plasma membrane (Fig. ) (Idänheimo et al ., ; Bourdais et al ., ; Lu et al ., ; Kimura et al ., ). This would be coupled to the activation of cytosolic kinases.…”

Section: H2o2 Signallingmentioning

confidence: 99%

Hydrogen peroxide metabolism and functions in plants

2018

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1 I. Introduction 2 II. Measurement and imaging of H O 2 III. H O and O toxicity 3 IV. Production of H O : enzymes and subcellular locations 4 V. H O transport 9 VI. Control of H O concentration: how and where? 9 VII. Metabolic functions of H O 11 VIII. H O signalling 11 IX. Where next? 13 Acknowledgements 13 References 13 SUMMARY: Hydrogen peroxide (H O ) is produced, via superoxide and superoxide dismutase, by electron transport in chloroplasts and mitochondria, plasma membrane NADPH oxidases, peroxisomal oxidases, type III peroxidases and other apoplastic oxidases. Intracellular transport is facilitated by aquaporins and H O is removed by catalase, peroxiredoxin, glutathione peroxidase-like enzymes and ascorbate peroxidase, all of which have cell compartment-specific isoforms. Apoplastic H O influences cell expansion, development and defence by its involvement in type III peroxidase-mediated polymer cross-linking, lignification and, possibly, cell expansion via H O -derived hydroxyl radicals. Excess H O triggers chloroplast and peroxisome autophagy and programmed cell death. The role of H O in signalling, for example during acclimation to stress and pathogen defence, has received much attention, but the signal transduction mechanisms are poorly defined. H O oxidizes specific cysteine residues of target proteins to the sulfenic acid form and, similar to other organisms, this modification could initiate thiol-based redox relays and modify target enzymes, receptor kinases and transcription factors. Quantification of the sources and sinks of H O is being improved by the spatial and temporal resolution of genetically encoded H O sensors, such as HyPer and roGFP2-Orp1. These H O sensors, combined with the detection of specific proteins modified by H O , will allow a deeper understanding of its signalling roles.

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Overexpression of an Arabidopsis cysteine-rich receptor-like protein kinase, CRK5, enhances abscisic acid sensitivity and confers drought tolerance

Abstract: HighlightThe cysteine-rich receptor-like protein kinase CRK5 is a potentially positive regulator of ABA signaling in early seedling growth, stomatal movement and plant drought tolerance.

Cited by 88 publications

References 62 publications

Genome-wide characterization of WRKY gene family in Helianthus annuus L. and their expression profiles under biotic and abiotic stresses

Genome-wide characterization of WRKY gene family in Helianthus annuus L. and their expression profiles under biotic and abiotic stresses

Genome-wide characterization of WRKY gene family in Helianthus annuus L. and their expression profiles under biotic and abiotic stresses

Hydrogen peroxide metabolism and functions in plants

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