Overexpression of a Harpin-encoding gene hrf1 in rice enhances drought tolerance

Zhang, Lei; Xiao, Shaobo; Li, Wenqi; Feng, Wei; Li, Juan; Wu, Zhidan; Gao, Xuewen; Liu, Fengquan; Shao, Min

doi:10.1093/jxb/err131

Cited by 106 publications

(66 citation statements)

References 50 publications

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“…Previous work revealed that expression of the harpin-encoding gene hrf1 in rice increased the ABA content and water retention ability and enhanced drought tolerance [34]. In the present study, the enhance tolerance to drought stress of MoHrip1- and MoHrip2-expressing rice might be related to the regulation of ABA biosynthesis and the capacity for water retention.…”

Section: Discussionsupporting

confidence: 57%

Enhanced disease resistance and drought tolerance in transgenic rice plants overexpressing protein elicitors from Magnaporthe oryzae

Wang

Han

et al. 2017

PLoS ONE

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Exogenous application of the protein elicitors MoHrip1 and MoHrip2, which were isolated from the pathogenic fungus Magnaporthe oryzae (M. oryzae), was previously shown to induce a hypersensitive response in tobacco and to enhance resistance to rice blast. In this work, we successfully transformed rice with the mohrip1 and mohrip2 genes separately. The MoHrip1 and MoHrip2 transgenic rice plants displayed higher resistance to rice blast and stronger tolerance to drought stress than wild-type (WT) rice and the vector-control pCXUN rice. The expression of salicylic acid (SA)- and abscisic acid (ABA)-related genes was also increased, suggesting that these two elicitors may trigger SA signaling to protect the rice from damage during pathogen infection and regulate the ABA content to increase drought tolerance in transgenic rice. Trypan blue staining indicated that expressing MoHrip1 and MoHrip2 in rice plants inhibited hyphal growth of the rice blast fungus. Relative water content (RWC), water usage efficiency (WUE) and water loss rate (WLR) were measured to confirm the high capacity for water retention in transgenic rice. The MoHrip1 and MoHrip2 transgenic rice also exhibited enhanced agronomic traits such as increased plant height and tiller number.

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Section: Discussionsupporting

confidence: 57%

Enhanced disease resistance and drought tolerance in transgenic rice plants overexpressing protein elicitors from Magnaporthe oryzae

Wang

Han

et al. 2017

PLoS ONE

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“…Stomatal apertures were measured as described previously [52]. Leaves of 3-week-old plants treated with 20% PEG6000 for 21 days were detached and immediately fixed by 2.5% glutaraldehyde in 0.1 M phosphate buffer (pH 7.2) at 4°C for 24 h, washed twice with 0.1 M phosphate buffer for 10 min, then immersed in 50, 60, 70, 80, 90, and 100% ethanol sequentially for 10 min each.…”

Section: Methodsmentioning

confidence: 99%

RNAi-Directed Downregulation of Vacuolar H+-ATPase Subunit A Results in Enhanced Stomatal Aperture and Density in Rice

et al. 2013

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Stomatal movement plays a key role in plant development and response to drought and salt stress by regulating gas exchange and water loss. A number of genes have been demonstrated to be involved in the regulation of this process. Using inverse genetics approach, we characterized the function of a rice (Oryza sativa L.) vacuolar H+-ATPase subunit A (OsVHA-A) gene in stomatal conductance regulation and physiological response to salt and osmotic stress. OsVHA-A was constitutively expressed in different rice tissues, and the fusion protein of GFP-OsVHA-A was exclusively targeted to tonoplast when transiently expressed in the onion epidermal cells. Heterologous expression of OsVHA-A was able to rescue the yeast mutant vma1Δ (lacking subunit A activity) phenotype, suggesting that it partially restores the activity of V-ATPase. Meanwhile, RNAi-directed knockdown of OsVHA-A led to a reduction of vacuolar H+-ATPase activity and an enhancement of plasma membrane H+-ATPase activity, thereby increasing the concentrations of extracellular H+ and intracellular K+ and Na+ under stress conditions. Knockdown of OsVHA-A also resulted in the upregulation of PAM3 (plasma membrane H+-ATPase 3) and downregulation of CAM1 (calmodulin 1), CAM3 (calmodulin 3) and YDA1 (YODA, a MAPKK gene). Altered level of the ion concentration and the gene expression by knockdown of OsVHA-A probably resulted in expanded aperture of stomatal pores and increased stomatal density. In addition, OsVHA-A RNAi plants displayed significant growth inhibition under salt and osmotic stress conditions. Taken together, our results suggest that OsVHA-A takes part in regulating stomatal density and opening via interfering with pH value and ionic equilibrium in guard cells and thereby affects the growth of rice plants.

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“…Monodehydroascorbate reductase is involved in elimination of H 2 O 2 in ascorbateglutathione cycle (Noctor and Foyer 1998), and plays a significant role in fighting oxidative stress induced by environmental factors (Sharma et al 2012;Caverzan et al 2016). Harpin-binding proteins are well known as elicitors that induce numerously responses in plants, such as hypersensitive response, systemic acquired resistance, reinforcement of growth, and tolerance to drought, and induce defense mechanism in response to nitrogen stress (Song et al 2010;Zhang et al 2011).…”

Section: Stress and Defense-related Proteinsmentioning

confidence: 99%

Proteomic signature of fenugreek treated by methyl jasmonate and cholesterol

et al. 2017

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Main conclusion Changes in proteome level as a result of methyl jasmonate and cholesterol treatment were investigated. The identified proteins were often involved in response to stress caused by various treatments. Furthermore, 18 proteins were expressed in treatmentspecific manner. In this study, the fenugreek plants were treated with methyl jasmonate (as an elicitor) and cholesterol (as a precursor of steroids and steroidal saponins) to check reaction at the level of the proteome to stress and to investigate steroidal saponin (diosgenin) biosynthesis. Proteins were separated by two-dimensional electrophoresis (2-DE) and identified by MALDI-ToF/ToF followed by database searches using Mascot search engine. Totally, 63 and 41 protein spots were differentially expressed after methyl jasmonate and cholesterol treatment, respectively. These proteins were classified into seven groups: photosynthesis, energy, metabolism, protein metabolism, secondary metabolism, stress and defense, and other. We found that 9 proteins were responsive to all treatments, and 18 proteins expressed in treatment-specific manner. Higher level of photosynthetic proteins sensitive to both biotic and abiotic stimuli was detected. In addition, proteins related to the stress (especially oxidative) and defense, protein, and secondary metabolism were overexpressed. The results indicate that methyl jasmonate and cholesterol elicited a defense reaction at the proteome level as a response to stress. The usefulness of 2-DE method for identification of proteins related with species-specific metabolic pathways is restricted. Integration of transcriptome data with proteomic analysis improved annotation process.

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Overexpression of a Harpin-encoding gene hrf1 in rice enhances drought tolerance

Cited by 106 publications

References 50 publications

Enhanced disease resistance and drought tolerance in transgenic rice plants overexpressing protein elicitors from Magnaporthe oryzae

Enhanced disease resistance and drought tolerance in transgenic rice plants overexpressing protein elicitors from Magnaporthe oryzae

RNAi-Directed Downregulation of Vacuolar H+-ATPase Subunit A Results in Enhanced Stomatal Aperture and Density in Rice

Proteomic signature of fenugreek treated by methyl jasmonate and cholesterol

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