Enhanced disease resistance and drought tolerance in transgenic rice plants overexpressing protein elicitors from Magnaporthe oryzae

Wang, Zhenzhen; Han, Qiang; Zi, Qian; Lv, Shun; Qiu, Dewen; Zeng, Hongmei

doi:10.1371/journal.pone.0175734

Cited by 25 publications

(18 citation statements)

References 49 publications

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“…During seed propagation of the LsNAC069 silenced lines, we observed an increased resistance to moderate drought stress. Recently, NACs were shown to be involved in drought stress and pathogen resistance (Wang et al ., ), therefore we tested the potential effect of drought stress on LsNAC069 nuclear relocalization. For this experiment, we used PEG treatment of the plant cells in a similar manner as the CF treatment (Figure b).…”

Section: Resultsmentioning

confidence: 99%

Multiple downy mildew effectors target the stress‐related NAC transcription factor LsNAC069 in lettuce

et al. 2019

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Summary To cause disease in lettuce, the biotrophic oomycete Bremia lactucae secretes potential RxLR effector proteins. Here we report the discovery of an effector‐target hub consisting of four B. lactucae effectors and one lettuce protein target by a yeast‐two‐hybrid (Y2H) screening. Interaction of the lettuce tail‐anchored NAC transcription factor, LsNAC069, with B. lactucae effectors does not require the N‐terminal NAC domain but depends on the C‐terminal region including the transmembrane domain. Furthermore, in Y2H experiments, B. lactucae effectors interact with Arabidopsis and potato tail‐anchored NACs, suggesting that they are conserved effector targets. Transient expression of RxLR effector proteins BLR05 and BLR09 and their target LsNAC069 in planta revealed a predominant localization to the endoplasmic reticulum. Phytophthora capsici culture filtrate and polyethylene glycol treatment induced relocalization to the nucleus of a stabilized LsNAC069 protein, lacking the NAC‐domain (LsNAC069ΔNAC). Relocalization was significantly reduced in the presence of the Ser/Cys‐protease inhibitor TPCK indicating proteolytic cleavage of LsNAC069 allows for relocalization. Co‐expression of effectors with LsNAC069ΔNAC reduced its nuclear accumulation. Surprisingly, LsNAC069 silenced lettuce lines had decreased LsNAC069 transcript levels but did not show significantly altered susceptibility to B. lactucae. In contrast, LsNAC069 silencing increased resistance to Pseudomonas cichorii bacteria and reduced wilting effects under moderate drought stress, indicating a broad role of LsNAC069 in abiotic and biotic stress responses.

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Section: Resultsmentioning

confidence: 99%

Multiple downy mildew effectors target the stress‐related NAC transcription factor LsNAC069 in lettuce

et al. 2019

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“…Defense relate proteins are also closely correlated with drought tolerance in plants. It has been reported that activation of plants defense against pathogens by expressing pathogen protein elicitors or R genes induces drought tolerance (Chini et al, 2004 ; Peng et al, 2015 ; Ma et al, 2017 ; Wang et al, 2017 ). Expression of Magnaporthe oryzae protein elicitors, Hypersensitive response-inducing protein 1 and 2 ( MoHrip1 and 2 ), can confer drought tolerance in rice together with enhanced blast resistance (Peng et al, 2015 ; Wang et al, 2017 ).…”

Section: Discussionmentioning

confidence: 99%

Overexpression of OsNAC14 Improves Drought Tolerance in Rice

et al. 2018

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Plants have evolved to have sophisticated adaptation mechanisms to cope with drought stress by reprograming transcriptional networks through drought responsive transcription factors. NAM, ATAF1-2, and CUC2 (NAC) transcription factors are known to be associated with various developmental processes and stress tolerance. In this study, we functionally characterized the rice drought responsive transcription factor OsNAC14. OsNAC14 was predominantly expressed at meiosis stage but is induced by drought, high salinity, ABA, and low temperature in leaves. Overexpression of OsNAC14 resulted in drought tolerance at the vegetative stage of growth. Field drought tests demonstrated that OsNAC14 overexpressing transgenic rice lines exhibited higher number of panicle and filling rate compared to non-transgenic plants under drought conditions. RNA-sequencing analysis revealed that OsNAC14 overexpression elevated the expression of genes for stress response, DNA damage repair, defense related, and strigolactone biosynthesis. In addition, chromatin immunoprecipitation analysis confirmed the direct interaction of OsNAC14 with the promoter of OsRAD51A1, a key component in homologous recombination in DNA repair system. Collectively, these results indicate that OsNAC14 mediates drought tolerance by recruiting factors involved in DNA damage repair and defense response resulting in improved tolerance to drought.

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“…Also other mite pests perform better on drought-stressed plants (Ximénez-Embún et al 2016 , 2017 ) and may become more serious pests in the future. To be ahead of this danger we should search for resistance traits that are displayed equally strong, or stronger, during drought, for reminiscent of, for example, the efforts for increasing diseases resistance together with drought tolerance in rice (Wang et al 2017 ), to protect tomato cultivation in the future.…”

Section: Discussionmentioning

confidence: 99%

Drought stress promotes the colonization success of a herbivorous mite that manipulates plant defenses

et al. 2017

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Climate change is expected to bring longer periods of drought and this may affect the plant’s ability to resist pests. We assessed if water deficit affects the tomato russet mite (TRM; Aculops lycopersici), a key tomato-pest. TRM thrives on tomato by suppressing the plant’s jamonate defenses while these defenses typically are modulated by drought stress. We observed that the TRM population grows faster and causes more damage on drought-stressed plants. To explain this observation we measured several nutrients, phytohormones, defense-gene expression and the activity of defensive proteins in plants with or without drought stress or TRM. TRM increased the levels of total protein and several free amino acids. It also promoted the SA-response and upregulated the accumulation of jasmonates but down-regulated the downstream marker genes while promoting the activity of cysteine—but not serine—protease inhibitors, polyphenol oxidase and of peroxidase (POD). Drought stress, in turn, retained the down regulation of JA-marker genes and reduced the activity of serine protease inhibitors and POD, and altered the levels of some free-amino acids. When combined, drought stress antagonized the accumulation of POD and JA by TRM and synergized accumulation of free sugars and SA. Our data show that drought stress interacts with pest-induced primary and secondary metabolic changes and promotes pest performance.Electronic supplementary materialThe online version of this article (10.1007/s10493-017-0200-4) contains supplementary material, which is available to authorized users.

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Enhanced disease resistance and drought tolerance in transgenic rice plants overexpressing protein elicitors from Magnaporthe oryzae

Cited by 25 publications

References 49 publications

Multiple downy mildew effectors target the stress‐related NAC transcription factor LsNAC069 in lettuce

Multiple downy mildew effectors target the stress‐related NAC transcription factor LsNAC069 in lettuce

Overexpression of OsNAC14 Improves Drought Tolerance in Rice

Drought stress promotes the colonization success of a herbivorous mite that manipulates plant defenses

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