Overexpression of a Brassica campestris HSP70 in tobacco confers enhanced tolerance to heat stress

Wang, Xiaorong; Yan, Bin; Shi, Min; Zhou, Wei; Zekria, David; Wang, Huizhong; Kai, Guoyin

doi:10.1007/s00709-015-0867-5

Cited by 54 publications

(30 citation statements)

References 56 publications

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“…Overexpression of an Hsp100 gene, PsHsp100, from Pleurotus sajor-caju complemented a thermotolerance defect in hsp104 mutant S. cerevisiae (Lee et al 2006). The expression increase of hmHsp70 is not as high as hsp70 genes in the cytosolic region (Wang et al 2016). The same result was also reported in S. cerevisiae, that the increase of SSC1 transcripts ranged from 1.5-to 4.0-fold after heat shock for 0.5 h (Craig et al 1987).…”

Section: Discussionsupporting

confidence: 82%

Heat shock protein 70 (HmHsp70) from Hypsizygus marmoreus confers thermotolerance to tobacco

Gao

Guo

et al. 2020

AMB Expr

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The 70-kD heat shock proteins (Hsp70s) have been proved to be important for stress tolerance and protein folding and unfolding in almost all organisms. However, the functions of Hsp70s in mushroom are not well understood. In the present study, a hsp70 gene from Hypsizygus marmoreus, hmhsp70, was cloned and transferred to tobacco (Nicotiana tabacum) to evaluate its function in thermotolerance. Sequence alignments and phylogenetic analysis revealed that HmHsp70 may be located in the mitochondria region. qPCR analysis revealed that the transcription level of hmhsp70 in H. marmoreus mycelia increased after heat shock treatment in high temperature (42 °C) compared with untreated mycelia (at 25 °C). Transgenic tobaccos expressing hmhsp70 gene showed enhanced resistance to lethal temperature compared with the wild type (WT) plants. Nearly 30% of the transgenic tobaccos survived after treated at a high temperature (50 °C and 52 °C for 4 h); however, almost all the WT tobaccos died after treated at 50 °C and no WT tobacco survived after heat shock at 52 °C. This study firstly showed the function of a hsp70 gene from H. marmoreus.

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Section: Discussionsupporting

confidence: 82%

Heat shock protein 70 (HmHsp70) from Hypsizygus marmoreus confers thermotolerance to tobacco

Gao

Guo

et al. 2020

AMB Expr

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“…Hsp70s are a class of highly conserved proteins which act as molecular chaperones and play a crucial role in protecting the plant cells from the harmful effects of heat stress. Hsp70 is known to accumulate in heat-stressed tissue and overexpression of Hsp70 was shown to enhance tolerance to heat stress in several plant species including brassica, tobacco and rice [ 27 , 28 , 29 , 30 ].…”

Section: Introductionmentioning

confidence: 99%

A High Temperature Environment Regulates the Olive Oil Biosynthesis Network

Nissim

Shlosberg

Biton

et al. 2020

Plants

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Climate change has been shown to have a substantial impact on agriculture and high temperatures and heat stress are known to have many negative effects on the vegetative and reproductive phases of plants. In a previous study, we addressed the effects of high temperature environments on olive oil yield and quality, by comparing the fruit development and oil accumulation and quality of five olive cultivars placed in high temperature and moderate temperature environments. The aim of the current study was to explore the molecular mechanism resulting in the negative effect of a high temperature environment on oil quantity and quality. We analyzed the transcriptome of two extreme cultivars, ‘Barnea’, which is tolerant to high temperatures in regard to quantity of oil production, but sensitive regarding its quality, and ‘Souri’, which is heat sensitive regarding quantity of oil produced, but relatively tolerant regarding its quality. Transcriptome analyses have been carried out at three different time points during fruit development, focusing on the genes involved in the oil biosynthesis pathway. We found that heat-shock protein expression was induced by the high temperature environment, but the degree of induction was cultivar dependent. The ‘Barnea’ cultivar, whose oil production showed greater tolerance to high temperatures, exhibited a larger degree of induction than the heat sensitive ‘Souri’. On the other hand, many genes involved in olive oil biosynthesis were found to be repressed as a response to high temperatures. OePDCT as well as OeFAD2 genes showed cultivar dependent expression patterns according to their heat tolerance characteristics. The transcription factors OeDof4.3, OeWRI1.1, OeDof4.4 and OeWRI1.2 were identified as key factors in regulating the oil biosynthesis pathway in response to heat stress, based on their co-expression characteristics with other genes involved in this pathway. Our results may contribute to identifying or developing a more heat tolerant cultivar, which will be able to produce high yield and quality oil in a future characterized by global warming.

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“…Especially, various reports to date reveal that SODs overexpressing plants protect them from oxidative damage [23][24][25][26]. Similarly, HSP overexpressing plants also showed excellent stress tolerance [27][28][29], which act as core genes in our interaction network analysis results. In addition, CYP family members also act as key response genes to drought stress [30,31].…”

Section: Discussionmentioning

confidence: 63%

De novo assembly of Amorpha fruticosa (Amorpha fruticosa L.) transcriptome in response to drought stress provides insight into the tolerance mechanisms

SUN

Liu

et al. 2019

Preprint

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Background Amorpha fruticosa (Amorpha fruticosa L.) is a deciduous shrub that is native to North America and has been introduced to China as an ornamental plant.In order to cultivate drought-tolerant Amorpha fruticosa varieties, it is important to understand the drought-tolerant mechanism of Amorpha fruticosa. Through the changes of the transcriptome of Amorpha fruticosa under drought stress, the mechanism of anti-stress of Amorpha fruticosa could be revealed. Different concentrations of polyethylene glycol-6000 (PEG-6000) was used to simulate drought stress, and transcriptomic analysis was used to reveal the changes of gene expression patterns in Amorpha fruticosa seedlings.Results Results showed that Amorpha fruticosa seedlings were seriously affected by PEG-6000. As for the differently expressed genes (DEGs), most of them were up-regulated. The additional Go and KEGG analysis results showed that DEGs were functionally enriched in cell wall, signal transduction and hormonal regulation related pathways. DEGs like AfSOD, AfHSP, AfTGA, AfbZIP and AfGRX play roles in response to drought stress.Conclusion In conclusion, Amorpha fruticosa seedlings were sensitive to drought, which was different from Amorpha fruticosa tree, and the genes functions in drought stress responses via ABA‐independent pathways. The up-regulation of Salicylic acid signal related DEGs (AfTGA and AfPR-1) indicated that Amorpha fruticosa can resist drought stress through Salicylic acid.

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Overexpression of a Brassica campestris HSP70 in tobacco confers enhanced tolerance to heat stress

Cited by 54 publications

References 56 publications

Heat shock protein 70 (HmHsp70) from Hypsizygus marmoreus confers thermotolerance to tobacco

Heat shock protein 70 (HmHsp70) from Hypsizygus marmoreus confers thermotolerance to tobacco

A High Temperature Environment Regulates the Olive Oil Biosynthesis Network

De novo assembly of Amorpha fruticosa (Amorpha fruticosa L.) transcriptome in response to drought stress provides insight into the tolerance mechanisms

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