Overexpressed IGFBP5 promotes cell proliferation and inhibits apoptosis of nucleus pulposus derived from rats with disc degeneration through inactivating the ERK/MAPK axis

Wang, Tao; Wang, Chunju; Shen, Tian; Song, Haibo

doi:10.1002/jcb.29191

Cited by 10 publications

(5 citation statements)

References 44 publications

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“…Gene pathway. The role of the MaPK pathway in the apoptosis of nP cells has previously been reported (30,31), and the MaPK pathway might be involved in the apoptosis observed in the present study. A DEG identified that is known to be involved with the MaPK pathway was cacna2d1, which could also indicate the potential role of cacna2d1 in the apoptosis of nP cells.…”

Section: Lps-stimulated Groups Syra-reversed Groups -----------------supporting

confidence: 71%

Gene expression profile of lipopolysaccharide‑induced apoptosis of nucleus pulposus cells reversed by syringic acid

et al. 2020

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apoptosis of nucleus pulposus (nP) cells has an important role in the process of intervertebral disc degeneration (idd), and the search for novel compounds to prevent apoptosis from occurring is urgently required. in the present study, syringic acid (Syra) was found to exhibit no cytotoxicity on nP cells, and was able to reverse the cytotoxicity, as well as the abnormal expression of Bcl-2 and caspase-3, that were induced by lipopolysaccharide (lPS). The transcriptomes of each group were then analyzed using rna-Seq. a total of 65 differentially expressed genes (deGs) were identified in LPS-stimulated groups (lPS group vs. control group), 819 DEGs were identified in the SyrA-reversed groups (Syra plus lPS group vs. lPS group), and a further 25 deGs were identified in the Syra plus lPS group compared with the control group. reverse transcription-quantitative Pcr validation indicated that the alterations in expression of uroplakin 3B-like 1 (uPK3Bl1), voltage-dependent calcium channel subunit α-2/δ-1 (cacna2d1) and polo-like kinase 4 (PlK4) were consistent with the corresponding results of rna-Seq, and that these genes were involved in both lPS-stimulation and Syra-reversion processes. Kyoto encyclopedia of Genes and Genomes analyses indicated that the deGs in Syra-reversed groups were involved in, amongst other pathways, 'autophagy-other' and 'apoptosis-multiple species'. in conclusion, the addition of Syra to the nP cells co-incubated with lPS appeared to help prevent the abnormal expression of mrnas and apoptosis that had been identified in nP cells incubated with lPS alone. The potential mechanism underlying the reversion of Syra might be attributed to the regulation of cacna2d1 and PlK4.

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Section: Lps-stimulated Groups Syra-reversed Groups -----------------supporting

confidence: 71%

Gene expression profile of lipopolysaccharide‑induced apoptosis of nucleus pulposus cells reversed by syringic acid

et al. 2020

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“…On the other hand, IGFBP5 antibody may inhibit cell proliferation and facilitate apoptosis of MSCs through activating the ERK/MAPK axis, which could be partly responsible for the decreased in ALP and Alizarin Red S staining. 37,38 At the same time, we can also conclude that MSC/POC ECM-based TEBs enhanced osteogenesis cannot be attributed entirely to IGFBP5 and there are other mechanisms, yet to be explored.…”

Section: Discussionmentioning

confidence: 74%

Engineered scaffolds based on mesenchymal stem cells/preosteoclasts extracellular matrix promote bone regeneration

et al. 2020

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Recently, extracellular matrix-based tissue-engineered bone is a promising approach to repairing bone defects, and the seed cells are mostly mesenchymal stem cells. However, bone remodelling is a complex biological process, in which osteoclasts perform bone resorption and osteoblasts dominate bone formation. The interaction and coupling of these two kinds of cells is the key to bone repair. Therefore, the extracellular matrix secreted by the mesenchymal stem cells alone cannot mimic a complex bone regeneration microenvironment, and the addition of extracellular matrix by preosteoclasts may contribute as an effective strategy for bone regeneration. Here, we established the mesenchymal stem cell/preosteoclast extracellular matrix -based tissue-engineered bones and demonstrated that engineered-scaffolds based on mesenchymal stem cell/ preosteoclast extracellular matrix significantly enhanced osteogenesis in a 3 mm rat femur defect model compared with mesenchymal stem cell alone. The bioactive proteins released from the mesenchymal stem cell/ preosteoclast extracellular matrix based tissue-engineered bones also promoted the migration, adhesion, and osteogenic differentiation of mesenchymal stem cells in vitro. As for the mechanisms, the iTRAQ-labeled mass spectrometry was performed, and 608 differentially expressed proteins were found, including the IGFBP5 and CXCL12. Through in vitro studies, we proved that CXCL12 and IGFBP5 proteins, mainly released from the preosteoclasts, contributed to mesenchymal stem cells migration and osteogenic differentiation, respectively. Overall, our research, for the first time, introduce pre-osteoclast into the tissue engineering of bone and optimize the strategy of constructing extracellular matrix–based tissue-engineered bone using different cells to simulate the natural bone regeneration environment, which provides new sight for bone tissue engineering.

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“…According to the previous genetic analysis of cluster 24, it was observed that IGFBP5 was highly expressed in the AM_Pain group. The gene IGFBP5 , which has been linked to neuropathic differentiation ( 35 ). Therefore, we suspected IGFBP5 might play a vital role in the process of multipotent stem cells differentiating into neurogenic cells.…”

Section: Resultsmentioning

confidence: 99%

SFRP4+IGFBP5hi NKT cells induced neural-like cell differentiation to contribute to adenomyosis pain

Chen

Zhu²,

Chen³

et al. 2022

Front. Immunol.

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BackgroundAdenomyosis is an estrogen-dependent gynecological disease. The pathogenesis of chronic pain, the main clinical symptom of adenomyosis, remains undefined. As a combination lymphocyte with both T-cell and natural killer (NK)–cell properties, NK T (NKT) cells play a role in immune defense against numerous diseases and modulate cell differentiation.MethodThis study analyzed the tissue-cell samples from adenomyosis with or without pain by single-cell sequencing.ResultWe found a specific population of secreted frizzled-related protein 4 (SFRP4)+NKT cells and a large amount of undifferentiated multipotent stem cells in the adenomyosis pain group. We discovered that a high expression of IGFBP5 in SFRP4+NKT cells could promote the differentiation of multipotent stem cells into neural-like cells via the single-cell trajectory. Through verification by the sample, we found that the degree of the expression of the neuronal marker NEFM was correlated with the duration of pain in adenomyosis patients. The expression of IGFBP5 was positively correlated with the pain scores of adenomyosis patients.ConclusionCollectively, these findings suggest that SFRP4+IGFBP5hi NKT cells were capable of converting part of the stem cells into neurogenic cells and inducing adenomyosis pain.

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Overexpressed IGFBP5 promotes cell proliferation and inhibits apoptosis of nucleus pulposus derived from rats with disc degeneration through inactivating the ERK/MAPK axis

Cited by 10 publications

References 44 publications

Gene expression profile of lipopolysaccharide‑induced apoptosis of nucleus pulposus cells reversed by syringic acid

Gene expression profile of lipopolysaccharide‑induced apoptosis of nucleus pulposus cells reversed by syringic acid

Engineered scaffolds based on mesenchymal stem cells/preosteoclasts extracellular matrix promote bone regeneration

SFRP4+IGFBP5hi NKT cells induced neural-like cell differentiation to contribute to adenomyosis pain

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