SFRP4+IGFBP5hi NKT cells induced neural-like cell differentiation to contribute to adenomyosis pain

Abstract

BackgroundAdenomyosis is an estrogen-dependent gynecological disease. The pathogenesis of chronic pain, the main clinical symptom of adenomyosis, remains undefined. As a combination lymphocyte with both T-cell and natural killer (NK)–cell properties, NK T (NKT) cells play a role in immune defense against numerous diseases and modulate cell differentiation.MethodThis study analyzed the tissue-cell samples from adenomyosis with or without pain by single-cell sequencing.ResultWe found a specific population of secreted frizzled-related protein 4 (SFRP4)+NKT cells and a large amount of undifferentiated multipotent stem cells in the adenomyosis pain group. We discovered that a high expression of IGFBP5 in SFRP4+NKT cells could promote the differentiation of multipotent stem cells into neural-like cells via the single-cell trajectory. Through verification by the sample, we found that the degree of the expression of the neuronal marker NEFM was correlated with the duration of pain in adenomyosis patients. The expression of IGFBP5 was positively correlated with the pain scores of adenomyosis patients.ConclusionCollectively, these findings suggest that SFRP4+IGFBP5hi NKT cells were capable of converting part of the stem cells into neurogenic cells and inducing adenomyosis pain.

Screening and identification of key biomarkers associated with endometriosis using bioinformatics and next generation sequencing data analysis

“…PCSK9 [48], CNTN4 [49], SEMA3A [50], SFRP4 [51], MFAP5 [52], BMP6 [53], CDH6 [54], PIEZO2 [55] and PKP2 [56] are a diagnostic markers for inflammation. PCSK9 [57], SEMA3A [58] and SFRP4 [59] are associated with pain. PCSK9 [60], CNTN4 [61], SEMA3A [62], PTGIS (prostaglandin I2 synthase) [63], SFRP4 [64], MFAP5 [65], CDH6 [66], GPC6 [67] and PKP2 [68] might serve as genetic markers of ovarian cancer.…”

Killer Cell Immunoglobulin-like Receptor Genotypes and Reproductive Outcomes in a Group of Infertile Women: A Romanian Study

“…The expression of inhibitory receptor CD94/NKG2A and NK cell expression was increased in patients with endometriosis. The presence of inhibitory phenotypic variants of NK cells in the ectopic endometrial tissue in adenomyosis has been equally reported, leading to the assumption that a lower cytotoxic potential of NK cells may facilitate the onset of adenomyosis/endometriosis [30][31][32]. In addition, in RIF patients the central disfunctions target the regulatory activity, expressed as altered angiogenic components (e.g., decreased VEGF and placental growth factor (PLGF)) [30].…”

“…In addition, in RIF patients the central disfunctions target the regulatory activity, expressed as altered angiogenic components (e.g., decreased VEGF and placental growth factor (PLGF)) [30]. In terms of representativity, the results of a meta-analysis clearly showed that the uterine NK cell number did not differ significantly between patients with or without RIF [31], thus underlining the importance of both the numeric representation and the functionality of NK cells [32].…”

Fibroblasts-derived migrasomes promote skin wound healing

“…Specifically, keratinocytes were predominant in clusters #3, #5, #6, #7, #9, and #10, characterized by markers such as Epcam, Krt14, and Krt15 29 . Immune cells were distributed across clusters #0, #1, #2, #8, #11, #12, #13, #14, #15, #16, and #18, with specific markers identifying neutrophils (cluster #0, #1, and #2) 32 , monocyte-macrophage lineage (cluster #8) 33 , dendritic cells (clusters #11 and #15) 34 , plasmacytoid dendritic cells (cluster #12) 35 , T cells and natural killer cells (cluster #13) 36 , natural killer T cells (cluster #14) 37 , and mast cells (cluster #18) 38 . Cluster #19 remained undefined because specific markers were lacking (Fig.…”