Overexpressed Hsa_circ_0001326 Contributes to the Decreased Cell Viability in SWAN71 Cells by Regulating MiR-186-5p/p27 Kip1 Axis

Tan, Juan; Zhong, Zhaoxia; Xu, Wen; Zhang, Naiyi

doi:10.1248/bpb.b20-00755

Cited by 7 publications

(6 citation statements)

References 21 publications

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“…Tan et al. reported that hsa_circ_0001326 regulates trophoblast cell function via absorbing miR‐186‐5p 19 . In this study, based on the microarray analysis and target prediction, we identified two candidate miRNAs, miR‐630 and miR‐145‐5p.…”

Section: Discussionmentioning

confidence: 90%

“…14,16,26 Tan et al reported that hsa_circ_0001326 regulates trophoblast cell function via absorbing miR-186-5p. 19 In this study, based on the microarray analysis and target prediction, we identified two candidate miRNAs, miR-630 and miR-145-5p. After inhibiting hsa_circ_0001326 in HTR-8/SVneo cells, the expression of miR-145-5p was promoted, while the expression of miR-630 had no significant change.…”

Section: Discussionmentioning

confidence: 99%

“…[14][15][16][17] There is evidence that hsa_circ_0001326 was increased in PE tissue and affected the viability of SWAN71 cells. 18,19 Another study showed that hsa_circ_0001326 curbs HTR-8/Svneo cell proliferation, epithelial-mesenchymal transition (EMT), migration, and invasion. 20 Therefore, hsa_circ_0001326 plays an important role in leading to PE.…”

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confidence: 99%

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Hsa_circ_0001326 inhibited the proliferation, migration, and invasion of trophoblast cells via miR‐145‐5p/TGFB2 axis

Song

Wang

et al. 2023

American J Rep Immunol

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Problem: Preeclampsia (PE) is an obstetric disease involving multiple systems, which account for maternal and fetal complications and increased mortality. Circular RNAs (circRNAs) were recently deemed to associate with the pathogenesis of PE. This study aims to clarify the correlation between circRNA hsa_circ_0001326 and PE and explore its biological function in PE. Method of study:The expression of hsa_circ_0001326 in PE placentas was detected by real-time quantitative PCR (qRT-PCR). After overexpressing or inhibiting hsa_circ_0001326 in trophoblast cells, the cell growth, migration, and invasion were evaluated by Cell Counting Kit-8 (CCK-8) and transwell assays. Western blot assay was applied to detect the epithelial-mesenchymal transition (EMT) proteins, E-cadherin and Vimentin. Furthermore, a dual-luciferase reporter assay was applied to verify the binding sites of hsa_circ_0001326, miR-145-5p, and transforming growth factor beta 2 (TGFB2).Results: Hsa_circ_0001326 was found to be higher expressed in PE placentas than in normal placentas. Furthermore, hsa_circ_0001326 played a negative regulating role in trophoblast cell viability, migration, and invasion. Overexpression of hsa_circ_0001326 inhibited the viability, migration, and invasion of trophoblast cells, while inhibition of hsa_circ_0001326 showed opposite effects. Mechanistically, hsa_circ_0001326 sponged miR-145-5p to elevate TGFB2 expression in trophoblast cells. Conclusion:This study provided evidence that the up-regulated hsa_circ_0001326 in PE restrained trophoblast cells proliferation, migration, and invasion by sponging miR-145-5p to elevate TGFB2 expression. Our results might provide a novel insight into the role of hsa_circ_0001326 in the pathogenesis of PE.

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Section: Discussionmentioning

confidence: 90%

Section: Discussionmentioning

confidence: 99%

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Hsa_circ_0001326 inhibited the proliferation, migration, and invasion of trophoblast cells via miR‐145‐5p/TGFB2 axis

Song

Wang

et al. 2023

American J Rep Immunol

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“…Moreover, as an effective tumor suppressor, p27 is widely involved in the regulation of tumors by non-coding RNAs. For instance, circ_0001326 regulates cell viability by affecting the expressions of p27, cyclin E1, and CDK2 [42]. Circ BCRC-3 acts as a sponge for miR-182-5p to up-regulate p27 expression, resulting in the suppression of bladder cancer cell proliferation [43].…”

Section: Discussionmentioning

confidence: 99%

Hypoxia-inducible CircPFKFB4 Promotes Breast Cancer Progression by Facilitating the CRL4^DDB2 E3 Ubiquitin Ligase-mediated p27 Degradation

Chen¹,

Yang²,

Liu³

et al. 2022

Int. J. Biol. Sci.

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Hypoxic microenvironment and circular RNAs (circRNAs) have shown critical implications in breast cancer (BC) progression. However, the specific functions and underlying mechanisms of circRNAs in BC under hypoxia remain largely unknown. We first screened for differentially expressed circRNAs in normoxic and hypoxic MCF-7 cells using circRNA microarray. A novel hypoxia-induced circRNA, circPFKFB4, was identified. Clinical investigation showed that circPFKFB4 was highly expressed in BC tissues and cell lines, and its overexpression was positively correlated with the advanced clinical stage and poor prognosis of BC patients. Functionally, circPFKFB4 promoted the proliferation of BC cells both in vitro and in vivo. Mechanistically, HIF1α bound to hypoxia response elements in the promoter region of the PFKFB4 gene to facilitate the biogenesis of circPFKFB4 under hypoxia. Hypoxia-induced circPFKFB4 directly bound to both DDB1 and DDB2 and promoted the CRL4 DDB2 E3 ubiquitin ligase assembly, resulting in p27 ubiquitination and BC progression under hypoxia. Our findings revealed a novel interaction between circPFKFB4 and the CRL4 DDB2 E3 ubiquitin ligase, suggesting that circPFKFB4 might serve as a promising biomarker and therapeutic target for BC.

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“…Circ_0001326 is originated from its parental gene Pleckstrin Homology Like Domain Family B Member 2 (PHLDB2) in chr3: 111632165–111639266, which was identified to be reinforced in placental tissues of PE [13]. Moreover, circ_0001326 up-regulation could induce the arrest of cell proliferation in SWAN71 trophoblast cell lines by elevating p27 Kip1 through miR-186-5p [14]. Besides that, Liu's team also disclosed that circ_0001326 up-regulation restrained HTR-8/Svneo cell dysfunction through miR-558/interleukin-16 axis [15].…”

Section: Introductionmentioning

confidence: 99%

Circ_0001326 suppresses trophoblast cell proliferation, invasion, migration and epithelial–mesenchymal transition progression in preeclampsia by miR-188-3p/HtrA serine peptidase 1 axis

Gao

Zhang

2023

Journal of Hypertension

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Background: It has been reported that the alteration of circular RNAs (circRNAs) during preeclampsia (PE) can be associated with the pathogenesis of this disease. Herein, this work investigated the potential functions and mechanism of circ_0001326 in PE process. Methods:The levels of genes and proteins were evaluated by quantitative real-time PCR (qRT-PCR) and western blotting. The functional experiments were conducted using cell counting kit-8 (CCK-8), 5-ethynyl-2'-deoxyuridine (EdU), flow cytometry, transwell, and wound healing assays, respectively. The binding between miR-188-3p and circ_0001326 or HtrA serine peptidase 1 (HTRA1) was verified by bioinformatics analysis and dual-luciferase reporter assays.Results: Circ_0001326 and HTRA1 expression was increased, while miR-188-3p expression was decreased in the placental tissues of preeclamptic singleton pregnant women compared with the normal pregnant women. Functionally, up-regulation of circ_0001326 or HTRA1, or down-regulation of miR-188-3p led to the arrest of cell growth, invasion, migration and epithelial-mesenchymal transition (EMT) process in trophoblast cells. Mechanistically, circ_0001326 acted as a sponge for miR-188-3p, which directly targeted HTRA1. Moreover, circ_0001326 could regulate HTRA1 through sequestering miR-188-3p. A series of rescue experiments showed that miR-188-3p reversed the inhibitory effects of circ_0001326 knockdown on above behaviors of trophoblast cells. Besides that, HTRA1 silencing attenuated the action of miR-188-3p inhibitor on trophoblast cell phenotype alteration. Conclusion:Our study demonstrated that circ_0001326 could promote trophoblast cell proliferation, invasion, migration and EMT in PE by miR-188-3p/HTRA1 axis, indicating a novel insight into the pathogenesis of PE.

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Overexpressed Hsa_circ_0001326 Contributes to the Decreased Cell Viability in SWAN71 Cells by Regulating MiR-186-5p/p27 Kip1 Axis

Cited by 7 publications

References 21 publications

Hsa_circ_0001326 inhibited the proliferation, migration, and invasion of trophoblast cells via miR‐145‐5p/TGFB2 axis

Hsa_circ_0001326 inhibited the proliferation, migration, and invasion of trophoblast cells via miR‐145‐5p/TGFB2 axis

Hypoxia-inducible CircPFKFB4 Promotes Breast Cancer Progression by Facilitating the CRL4^DDB2 E3 Ubiquitin Ligase-mediated p27 Degradation

Circ_0001326 suppresses trophoblast cell proliferation, invasion, migration and epithelial–mesenchymal transition progression in preeclampsia by miR-188-3p/HtrA serine peptidase 1 axis

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Overexpressed Hsa_circ_0001326 Contributes to the Decreased Cell Viability in SWAN71 Cells by Regulating MiR-186-5p/p27 Kip1 Axis

Cited by 7 publications

References 21 publications

Hsa_circ_0001326 inhibited the proliferation, migration, and invasion of trophoblast cells via miR‐145‐5p/TGFB2 axis

Hsa_circ_0001326 inhibited the proliferation, migration, and invasion of trophoblast cells via miR‐145‐5p/TGFB2 axis

Hypoxia-inducible CircPFKFB4 Promotes Breast Cancer Progression by Facilitating the CRL4DDB2 E3 Ubiquitin Ligase-mediated p27 Degradation

Circ_0001326 suppresses trophoblast cell proliferation, invasion, migration and epithelial–mesenchymal transition progression in preeclampsia by miR-188-3p/HtrA serine peptidase 1 axis

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Hypoxia-inducible CircPFKFB4 Promotes Breast Cancer Progression by Facilitating the CRL4^DDB2 E3 Ubiquitin Ligase-mediated p27 Degradation