Over Expression of Stem Cell Homing Cytokines in Urogenital Organs Following Vaginal Distention

Woo, Lynn L.; Hijaz, Adonis; Kuang, Mei; Penn, Marc S.; Damaser, Margot S.; Rackley, Raymond R.

doi:10.1016/j.juro.2006.11.047

Cited by 43 publications

(65 citation statements)

References 19 publications

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“…9,15 Our findings confirm our previously reported observations showing increased expression of MCP-3 in the urethra immediately after VD. 4 However, we did not confirm the prior observation of SDF-1 downregulation after VD. This may be due, in part, because we did not include unanesthetized control or sham groups, since Woo et al found that MCP-3 expression was significantly increased 20-fold in both shams and controls.…”

Section: Discussioncontrasting

confidence: 90%

“…This may be due, in part, because we did not include unanesthetized control or sham groups, since Woo et al found that MCP-3 expression was significantly increased 20-fold in both shams and controls. 4 We observed a significant trend in MCP-3 expression in rectal tissue after VD of increasing durations, suggesting that stem cell homing could play a role in rectal healing after delivery, a risk factor for fecal incontinence. 16 These findings could therefore indicate that treatments or preventatives based on facilitating stem cell homing may be applicable to fecal incontinence as well as SUI.…”

Section: Discussionmentioning

confidence: 61%

“…[2][3][4] Previous studies have demonstrated increased tissue damage with increasing duration of VD. 3,5 We have previously demonstrated that one stem cell homing factor, monocyte chemotactic protein-3 (MCP3), is upregulated in vaginal and urethral tissues immediately after VD in virgin rats.…”

Section: Introductionmentioning

confidence: 99%

“…3,5 We have previously demonstrated that one stem cell homing factor, monocyte chemotactic protein-3 (MCP3), is upregulated in vaginal and urethral tissues immediately after VD in virgin rats. 4 However, studies comparing molecular markers of tissue repair after VD of differing durations are lacking.…”

Section: Introductionmentioning

confidence: 99%

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Cytokine Expression After Vaginal Distension of Different Durations in Virgin Sprague Dawley Rats

Wood¹,

Kuang²,

Woo³

et al. 2008

Journal of Urology

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Purpose-To investigate the effect of duration of vaginal distention (VD) on the differential expression of stem cell homing and tissue repair cytokines and cytokine receptors in order to identify factors most important for recovery from injury.Methods-Twenty 10-week-old virgin Sprague-Dawley rats were divided into 4 groups: 1-hour VD, 4-hour VD, 6-hour VD, and anesthetized shams. Vagina, bladder, urethra and rectum were harvested immediately after VD and Real-Time PCR was used to determine relative expression of cytokines and receptors of interest. Mixed models analysis was used to determine associations between expression level and VD duration.Results-Positive associations between VD duration and level of urethral expression were found for one of the receptors of MCP-3 [CCR1 (p=0.0001)], as well as, for MCP-3 (p=0.025), CCR5 (p=0.032), and HIF1-alpha, (p=0.023). A positive relationship between VD duration and MCP-3 expression was also observed in rectal tissue (p=0.035). Urethral expression of CCR2, another receptor for MCP-3, approached significance (p=0.066). An inverse relationship between VD duration and expression of IL8 was found in the bladder (p=0.0008). No association was demonstrated between VD duration and expression of SDF-1, CXCR4, CCR2, CCR3, and VEGF in any pelvic organs.Conclusions-These data support a relationship between VD duration and subsequent expression of MCP-3 and one of its associated receptors (CCR1) in the urethra immediately following VD. The increase in HIF1-alpha expression in the urethra with prolonged VD suggests a limited role of tissue ischemia in the immediate response of pelvic organs to VD.

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Section: Discussioncontrasting

confidence: 90%

Section: Discussionmentioning

confidence: 61%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Cytokine Expression After Vaginal Distension of Different Durations in Virgin Sprague Dawley Rats

Wood¹,

Kuang²,

Woo³

et al. 2008

Journal of Urology

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“…A higher prevalence or incidence of SUI has been observed in women who had at least one vaginal delivery than in women who underwent cesarean delivery [13][14][15][16][17][18]. There is 20-fold over expression of macrophage chemoattractant protein 3 in the urethra immediately following vaginal delivery, which has been postulated to be secondary to a shear effect on the pelvic muscles [19]. Vaginal delivery has been correlated with SUI development, likely via injury of the pelvic floor structures: muscles, nerves, organs, and extracellular matrix (ECM) responsible for continence [20].…”

Section: Neuromuscularmentioning

confidence: 99%

Postpartum stress urinary incontinence, is it related to vaginal delivery?

Wang

Ghoniem

2017

The Journal of Maternal-Fetal & Neonatal Medicine

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Simulated childbirth injuries in an inbred rat strain

Woo

Hijaz

Pan

et al. 2008

Neurourology and Urodynamics

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Vaginal distension (VD) in outbred rats has been shown to decrease urethral resistance, as well as increase the expression of the stem cell-homing chemokine, monocyte chemotactic factor 3 (MCP-3), but not stromal derived factor 1 (SDF-1). The aim of this study was to determine if similar responses are induced by VD in an inbred rat strain. Forty female Lewis rats underwent VD or sham VD followed by leak point pressure (LPP) testing 4 or 10 days later. Ten additional rats served as controls. The urethra and vagina were then dissected for histology. To examine chemokine expression, 8 additional rats underwent VD with organs harvested immediately or 1 day after the procedure for RT-PCR of MCP-3 and SDF-1. Four age-matched rats served as controls. Four days after VD, LPP was significantly lower in VD rats (14.3±1.6 cmH 2 O) than controls (18.7±1.3 cmH 2 O). Ten days after VD, LPP in both VD (19.7±2.6 cmH 2 O) and sham (18.4±1.3 cmH 2 O) groups was not significantly different from controls. Urethral histology demonstrated marked disruption and atrophy of smooth and striated muscle in VD rats compared to shams and controls. RT-PCR yielded a 25-fold significant increase in expression of urethral MCP-3 immediately following VD. SDF-1 was significantly decreased in the urethra and vagina immediately after VD and in the bladder 24 hours after VD. In conclusion, VD in Lewis rats produces functional, histological and molecular results similar to that of outbred rats. This model could be utilized in future studies investigating cellular transplant methods of improving urethral function.

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Over Expression of Stem Cell Homing Cytokines in Urogenital Organs Following Vaginal Distention

Cited by 43 publications

References 19 publications

Cytokine Expression After Vaginal Distension of Different Durations in Virgin Sprague Dawley Rats

Cytokine Expression After Vaginal Distension of Different Durations in Virgin Sprague Dawley Rats

Postpartum stress urinary incontinence, is it related to vaginal delivery?

Simulated childbirth injuries in an inbred rat strain

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