Over-expression of OsPT2 under a rice root specific promoter Os03g01700

Li, Yuanya; Li, Caixia; Lizhong, Cheng; Yu, Shuang-Shuang; Shen, Chenjia; Pan, Yue

doi:10.1016/j.plaphy.2019.01.009

Cited by 10 publications

(9 citation statements)

References 32 publications

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“…Ratio greater than 1: gene up-regulated in transgenic plants; Ratio less than 1: gene down-regulated in transgenic plants; Ratio equal to 1: gene was not induced in transgenic plants (abbreviated as PHR1 below) is considered as the strategy of plants to cope with low-phosphorus stress [6,7]. On the other hand, previous studies of phosphorus transporter genes found that the level of phosphorus content in the roots was not the decisive factor to determine the root architectural alteration in response to low-phosphorus stress [22,23]. It is speculated that PHR1 regulates root development through an unknown pathway independently of the plant tissue phosphorus level.…”

Section: Discussionmentioning

confidence: 99%

EoPHR2, a Phosphate Starvation Response Transcription Factor, is Involved in Improving Low-Phosphorus Stress Resistance in Eremochloa ophiuroides

Chen¹,

Liu²,

He³

et al. 2022

Phyton

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As a macronutrient, Phosphorus (P) takes many roles in plant growth and development. It should be significant to explore the molecular mechanism of low-phosphorus stress response of plants. Phosphate starvation response (PHR) transcription factors play important roles in response to phosphorus deficiency stress in plants. In this study, we isolated a gene related to the plant phosphorus signaling system from the acid-soil-resistant centipedegrass (Eremochloa ophiuroides [Munro] Hack.), termed EoPHR2. The subcellular localization of EoPHR2 protein was observed to be nuclear located. The expression patterns of EoPHR2 in different tissues and Al/Pi-stress conditions were analyzed by qRT-PCR, they suggested a potential role in response to the multiple-stress under acid soil adversity. Based on the functional identification through transgenic plants, we found that (1) EoPHR2 is involved in the Pi-signaling pathway, and (2) overexpression of EoPHR2 mimics Pi-starvation signalling resulting on enhanced roots whether under Pi-deficiency stress or not. In conclusion, EoPHR2 transcription factor plays a role in response to the multiple stresses under acid soil conditions, improving the low-phosphorus stress resistance of Eremochloa ophiuroides.

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Section: Discussionmentioning

confidence: 99%

EoPHR2, a Phosphate Starvation Response Transcription Factor, is Involved in Improving Low-Phosphorus Stress Resistance in Eremochloa ophiuroides

Chen¹,

Liu²,

He³

et al. 2022

Phyton

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“…For example, CDR engineering involves the modification of plant form, such as changing root architecture with less nodal root number and more deep roots in maize [176], which requires precise control of gene expression by tissue-specific promoters [177]. Leaf-specific promoters [178] can be used for driving the expression of genes involved in CO 2 fixation; phloem tissuespecific promoters [179] can be used for genes involved in phloem-mediated translocation of sugars; and root-specific promoters [180,181] can be used for genes involved in root growth and development. Besides tissue-specific promoters, cell-type-specific promoters [182,183] can be used for highprecision control of the spatial expression pattern of CDRrelated genes, Also, to optimize the performance of a plant system for CDR, it is necessary to fine-tune the expression of genes involved in different processes (e.g., CO 2 fixation, carbon partitioning and translocation, and carbon storage) to achieve an optimal balance between source and sink activities.…”

Section: Discussionmentioning

confidence: 99%

Biological Parts for Plant Biodesign to Enhance Land-Based Carbon Dioxide Removal

Yang

Liu

et al. 2021

BioDesign Res

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A grand challenge facing society is climate change caused mainly by rising CO2 concentration in Earth’s atmosphere. Terrestrial plants are linchpins in global carbon cycling, with a unique capability of capturing CO2 via photosynthesis and translocating captured carbon to stems, roots, and soils for long-term storage. However, many researchers postulate that existing land plants cannot meet the ambitious requirement for CO2 removal to mitigate climate change in the future due to low photosynthetic efficiency, limited carbon allocation for long-term storage, and low suitability for the bioeconomy. To address these limitations, there is an urgent need for genetic improvement of existing plants or construction of novel plant systems through biosystems design (or biodesign). Here, we summarize validated biological parts (e.g., protein-encoding genes and noncoding RNAs) for biological engineering of carbon dioxide removal (CDR) traits in terrestrial plants to accelerate land-based decarbonization in bioenergy plantations and agricultural settings and promote a vibrant bioeconomy. Specifically, we first summarize the framework of plant-based CDR (e.g., CO2 capture, translocation, storage, and conversion to value-added products). Then, we highlight some representative biological parts, with experimental evidence, in this framework. Finally, we discuss challenges and strategies for the identification and curation of biological parts for CDR engineering in plants.

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“…Tissue-specific promoters are generally a 5′-UTR of genes expressed in specific tissues, organs, or specific development stages, which can effectively avoid negative effects on plant growth and development [ 1 ]. Although some tissue-specific expression promoters have been cloned, mostly including root [ 8 ], leaf sheath [ 9 ], phloem [ 10 ], pollen grain [ 11 ], embryo [ 12 ], endosperm [ 13 ] and green tissue [ 14 , 15 , 16 , 17 ], but only a small part of cis -acting regulatory elements are recognized [ 18 ]. In addition, the cloned green tissue-specific expression promoters are mainly some genes closely related to light induction and photosynthetic system, which participate in plant photosynthesis and realize the function of converting light energy into sugar [ 19 ], such as RBCs [ 20 ], DX1 [ 14 ], D540 [ 21 ].…”

Section: Introductionmentioning

confidence: 99%

Isolation and Functional Characterization of a Green-Tissue Promoter in Japonica Rice (Oryza sativa subsp. Japonica)

Lin

Yan

Ali

et al. 2022

Biology

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Plant promoters play a vital role in the initiation and regulation of gene transcription. In this study, a rice protein/gene of unknown expression, named Os8GSX7, was gained from a rice T-DNA capture line. The semi-quantitative RT-PCR analysis showed that the gene was only expressed in root, glume, and flower, but not in stem, leaf, embryo, and endosperm of japonica rice. The GUS activity analysis of the GSX7R promoter showed that it was a reverse green tissue expression promoter, except in endosperm. The forward promoter of GSX7 cannot normally drive the expression of the foreign GUS gene, while the reverse promoter of GSX7 is a green tissue-specific expression promoter, which can drive the expression of the foreign GUS gene. The region from −2097 to −1543 bp was the key region for controlling the green tissue-specific expression. The regulatory sequences with different lengths from the 2097 bp reverse sequence from the upstream region of the Os8GSX7 were fused with the GUS reporter gene and stably expressed in rice. Furthermore, transgenic rice plants carrying Cry1Ab encoding Bacillus thuringiensis endotoxin, regulated by GSX7R, were resistant to yellow stem borer. The analysis suggested that 10 light responsive elements of tissue-specific expression were found, including ACE, Box4, CAT-box, G-Box, G-box, GATA motif, GC motif, I-box, Sp1, and chs-unit1 M1. In addition, the results of 5′ and 3′ deletions further speculated that ACE and I-box may be the key elements for determining the green tissue-specific expression of GSX7R promoter.

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Over-expression of OsPT2 under a rice root specific promoter Os03g01700

Cited by 10 publications

References 32 publications

EoPHR2, a Phosphate Starvation Response Transcription Factor, is Involved in Improving Low-Phosphorus Stress Resistance in Eremochloa ophiuroides

EoPHR2, a Phosphate Starvation Response Transcription Factor, is Involved in Improving Low-Phosphorus Stress Resistance in Eremochloa ophiuroides

Biological Parts for Plant Biodesign to Enhance Land-Based Carbon Dioxide Removal

Isolation and Functional Characterization of a Green-Tissue Promoter in Japonica Rice (Oryza sativa subsp. Japonica)

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