Over-Expression of Oct4 in Human Esophageal Squamous Cell Carcinoma

Zhou, Xi; Huang, Guang-Rong; Hu, Pin

doi:10.1007/s10059-011-2315-5

Cited by 36 publications

(23 citation statements)

References 37 publications

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“…A previous study showed that overexpression of OCT4 in ESCC has been consistently associated with cancer proliferation, metastasis, and drug resistance 22. In the present study, based on the analysis of 95 ESCC patient samples with complete follow‐up data, the LEF1 expression level in ESCC was higher than that in adjacent tissue, and the high expression of LEF1 in patients was strongly associated with poor prognosis.…”

Section: Discussionsupporting

confidence: 47%

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Prognostic value of association of OCT4 with LEF1 expression in esophageal squamous cell carcinoma and their impact on epithelial‐mesenchymal transition, invasion, and migration

Zhao

Huang

et al. 2018

Cancer Medicine

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Esophageal squamous cell carcinoma (ESCC) is a malignant disease with poor prognosis. Because of early metastasis prior to diagnosis and therapeutic resistance, ESCC has become one of the leading causes of cancer‐related death. Here, we investigated the clinicopathological significance of the association of octamer‐binding transcription factor 4 (OCT4) with lymphoid enhancer‐binding factor 1 (LEF1) expression and the potential molecular mechanism in the epithelial‐mesenchymal transition (EMT), invasion, and migration of ESCC. The expression of OCT4 and LEF1 was detected via immunohistochemistry analysis. High levels of LEF1 expression were observed in 95 ESCC specimens and were obviously associated with aberrant clinicopathological features and poor patient prognosis. Our previous study showed that OCT4 expression level is elevated in ESCC, and statistical analysis showed that the elevated expression of OCT4 and LEF1 in ESCC was significantly associated with histologic grade, lymph node metastasis, TNM stage, and poor patient prognosis. The specific inhibition of OCT4 expression via a lentivirus encoding OCT4‐shRNA (LV‐shOCT4) in Eca109 cells led to decreased levels of OCT4 and LEF1 in vitro. Additionally, we applied a rescue strategy by infecting LV‐shOCT4 Eca109 cells with a LEF1 overexpression plasmid (p‐LEF1) and detected changes in EMT, migration, and invasion. Unsurprisingly, the p‐LEF1 group exhibited greater EMT, invasion, and migration than did the LV‐shOCT4 and negative control groups. This study demonstrates for the first time the relationship between OCT4 and LEF1 expression. The combination of high expression of OCT4 and LEF1 was associated with clinicopathological features of atypical patients, and this combination might be an ideal prognostic factor in ESCC. OCT4 positively regulated LEF1 expression, and LEF1 mediated the effects of OCT4 in cancer cell EMT, invasion, and migration. The data presented here suggest that the inhibition of OCT4‐LEF1 signaling may be a new therapeutic target for the treatment of ESCC.

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Section: Discussionsupporting

confidence: 47%

“…Previous studies have shown that the expression of OCT4 is increased to a variable extent in tumor specimens from patients with ESCC 22. However, no studies have reported on LEF1 expression and its relationship with clinicopathological features in ESCC.…”

Section: Resultsmentioning

confidence: 96%

Prognostic value of association of OCT4 with LEF1 expression in esophageal squamous cell carcinoma and their impact on epithelial‐mesenchymal transition, invasion, and migration

Zhao

Huang

et al. 2018

Cancer Medicine

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“…Previous studies have found that Sox2 might promote cell proliferation and tumorigenesis of breast cancer [10], and Oct3/4 might associated with the early stage of pancreatic cancer carcinogenesis [11,12], and even correlated with lymph node metastasis in colorectal cancer cells [13]. High expression of OCT-4 has been observed in esophageal cancer [14], suggesting association between OCT-4 and proliferation of esophageal cancer.…”

Section: Introductionmentioning

confidence: 93%

Increased Nanog Expression Promotes Tumor Development and Cisplatin Resistance in Human Esophageal Cancer Cells

Li¹,

Zhang²,

Zhang³

et al. 2012

Cell Physiol Biochem

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Background/Aims: Nanog plays a key role in stem cell self-renewal and pluripotency differentiation in embryonic stem cells ( ESCs). Recently, some studies reported that abnormal expression of Nanog could be detected in several tumors, indicating that Nanog might be related to tumor development. However, studies on the correlation between Nanog expression and esophageal cancer are sparse. Methods: In this study, we established two esophageal cancer cell lines 9706-Nanog and 9706-shNanog which stably expressed Nanog and Nanog-short-hairpin RNA (shRNA) genes. Results: We found that Nanog expression could promote the proliferation and invasiveness of the cancer cells, and inhibit the apoptosis. We also treated 9706-Nanog, EC9706 and 9706-shNanog cell lines with cisplatin and evaluated the drug sensitivity of the three cell lines. We found that the sensitivity of cisplatin was decreased with increased expression of Nanog. The expression of MDR-1 was also increased in 9706Nanog cells. Conclusions: Nanog may play an important role in human esophageal cancer development, and could be used as a therapeutic target in esophageal cancer treatment.

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“…18,19 There have been studies that reported that Oct4 is overexpressed in many kinds of cancers, such as, lung cancer, oral squamous cell carcinoma, GC, breast cancer, and esophageal cancer and ectopic expression may play a role in the improvement of disease. [20][21][22][23] Nevertheless, there is little relation between MUC5AC and Oct4 with clinical factors in GC, and contradictory findings have been reported. In addition to clinical outcome, lymph node and distant metastasis has been shown to be important factors for prognostic in GC.…”

Section: In Addition Low Expression Of Muc5ac and High Expression Ofmentioning

confidence: 99%

Differential expression of two genes Oct‐4 and MUC5AC associates with poor outcome in patients with gastric cancer

Javanbakht

Akhavan-Moghadam

Talaei

et al. 2017

Clin Exp Pharma Physio

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Gastric cancer (GC) is the most frequent leading cause of cancer-associated mortality worldwide that is linked to poor prognosis due to the lack of appropriate biomarkers. Our aim was to evaluate the MUC5AC and Oct-4 expression levels in GC and to assess their association with clinical factors. Immunohistochemical analysis (IHC) and qRT-PCR were performed in GC patients to examine the MUC5AC and Oct-4 expression levels. The mRNA level of MUC5AC was significantly decreased in tumour tissues compared with non-cancerous tissues (1.11 ± 0.69 vs 3.7 ± 0.71; P = .024). On the other hand, Oct-4 mRNA level was upregulated in tumour tissues as compared to normal tissues (2. 86 ± 0.78 vs 0.87 ± 0.54; P = .0015). Decreased expression of MUC5AC was detected in 27 patients (67.5%), while high to moderate expression levels were observed in 13 cases (32.5%), but in normal tissues the expression levels of MUC5AC were increased (P = .001). The decreased expression of MUC5AC was associated with aggressive tumour characteristics, such as TNM stage (P = .023), histologic type (P = .012) and lymph node metastasis (P = .001). High expression of Oct-4 was detected in 24 tumour tissues (60%), while 16 cases (40%) showed low expression level. Increased Oct-4 expression was correlated with clinicopathological characteristics such TNM stage (P = .002), histologic type (P = .008) and lymph node metastasis (P = .001). Our results showed that high Oct-4 expression and the reduction of MUC5AC expression may be involved in the progression and an unfavorable prognosis of GC.

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Over-Expression of Oct4 in Human Esophageal Squamous Cell Carcinoma

Cited by 36 publications

References 37 publications

Prognostic value of association of OCT4 with LEF1 expression in esophageal squamous cell carcinoma and their impact on epithelial‐mesenchymal transition, invasion, and migration

Prognostic value of association of OCT4 with LEF1 expression in esophageal squamous cell carcinoma and their impact on epithelial‐mesenchymal transition, invasion, and migration

Increased Nanog Expression Promotes Tumor Development and Cisplatin Resistance in Human Esophageal Cancer Cells

Differential expression of two genes Oct‐4 and MUC5AC associates with poor outcome in patients with gastric cancer

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