Over-expression of a NAC 67 transcription factor from finger millet (Eleusine coracana L.) confers tolerance against salinity and drought stress in rice

Rahman, Hazir; Ramanathan, V.; Nallathambi, Jagedeeshselvam; Sudhakar, D.; Raveendran, M.

doi:10.1186/s12896-016-0261-1

Cited by 113 publications

(48 citation statements)

References 59 publications

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“…Genes encoding transcription factor(s) and/or those involved in abiotic stress response, including bZIP ( LOC_Os08g36790 ), zinc finger ( LOC_Os08g38460 ), AP2 ( LOC_Os04g57340 ), no apical meristem ( LOC_Os02g38130 ), and homeobox ( LOC_Os01g06560 ), were found to be correlated with hypomethylation and higher gene expression under desiccation stress in Nagina 22. Gain of function of bZIP ( OsbZIP71 ), zinc finger ( OsSAP1 ), a member of APETELLA 2 ( OsAP37 ) and no apical meristem (NAM) transcription factors conferred tolerance to desiccation stress in rice (Mukhopadhyay et al ., 2004; Oh et al ., 2009; Liu et al ., 2014; Rahman et al ., 2016). A gene involved in photochemical quenching and dissipation of excess light, zeaxanthin epoxidase gene ( LOC_Os04g37619 ), showed hypomethylation in CHH context and higher expression.…”

Section: Resultsmentioning

confidence: 99%

Role of DNA methylation dynamics in desiccation and salinity stress responses in rice cultivars

Rajkumar

Shankar

Garg

et al. 2019

Preprint

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Word count: 5338 25 26Highlight: Bisulphite sequencing revealed single base resolution DNA methylation, and 27 cultivar-specific differential methylation patterns and correlation with gene expression that 28 control desiccation and salinity stress response in the rice cultivars. 29 30 2 Abstract 31 DNA methylation is an epigenetic mark that controls gene expression in response to internal and 32 environmental cues. In this study, we sought to understand the role of DNA methylation in 33 response to desiccation and salinity stresses in three rice cultivars (IR64, stress-sensitive; 34Nagina 22, drought-tolerant and Pokkali, salinity-tolerant) via bisulphite sequencing. We 35 identified DNA methylation patterns in different genomic/genic regions and analysed their 36 correlation with gene expression. Methylation in CG context within gene body and methylation 37 in CHH context in distal promoter regions were positively correlated with gene expression. 38However, methylation in other sequence contexts and genic regions was negatively correlated 39 with gene expression. DNA methylation was found to be most dynamic in CHH context under 40 stress condition(s) in the rice cultivars. The expression profiles of genes involved in de-novo 41 methylation were correlated with methylation dynamics. Hypomethylation in Nagina 22 and 42 hypermethylation in Pokkali in response to desiccation and salinity stress, respectively, were 43 correlated with higher expression of abiotic stress response related genes. Our results suggest an 44 important role of DNA methylation in abiotic stress responses in rice in cultivar-specific 45 manner. This study provides useful resource of DNA methylomes that can be integrated with 46 other data to understand abiotic stress response in rice. 47 48 49

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Section: Resultsmentioning

confidence: 99%

Role of DNA methylation dynamics in desiccation and salinity stress responses in rice cultivars

Rajkumar

Shankar

Garg

et al. 2019

Preprint

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“…Arabidopsis ANAC092 (also known as AtNAC2 or ORE1) is associated with the regulation of ethylene and hormone signaling, and overexpression can alter lateral root number, growth, and development [21]. Overexpression of the millet TF OsNAC67 can increase rice tolerance to high salt and drought [22], while overexpression of ZmSNAC1 can enhance the tolerance of maize to drought [23].…”

Section: Introductionmentioning

confidence: 99%

Knockout of the OsNAC006 Transcription Factor Causes Drought and Heat Sensitivity in Rice

Wang

Zhong

Wang

et al. 2020

IJMS

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Rice (Oryza sativa) responds to various abiotic stresses during growth. Plant-specific NAM, ATAF1/2, and CUC2 (NAC) transcription factors (TFs) play an important role in controlling numerous vital growth and developmental processes. To date, 170 NAC TFs have been reported in rice, but their roles remain largely unknown. Herein, we discovered that the TF OsNAC006 is constitutively expressed in rice, and regulated by H2O2, cold, heat, abscisic acid (ABA), indole-3-acetic acid (IAA), gibberellin (GA), NaCl, and polyethylene glycol (PEG) 6000 treatments. Furthermore, knockout of OsNAC006 using the CRISPR-Cas9 system resulted in drought and heat sensitivity. RNA sequencing (RNA-seq) transcriptome analysis revealed that OsNAC006 regulates the expression of genes mainly involved in response to stimuli, oxidoreductase activity, cofactor binding, and membrane-related pathways. Our findings elucidate the important role of OsNAC006 in drought responses, and provide valuable information for genetic manipulation to enhance stress tolerance in future plant breeding programs.

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“…Subgroups 8, 16 and 18 contain mostly unknown NACs, where the function has not been described yet. There are only four genes previously described, ANAC005 involved in xylem formation (45), NTM1 involved in cell division (46) and two genes involved in abiotic stress, NTM2 and NAC67 (47, 48). Quinoa has a high tolerance to salinity and water deficit; it is possible, that the absence of these genes contributes to quinoa’s tolerance.…”

Section: Resultsmentioning

confidence: 99%

Genome wide identification of NAC transcription factors and their role in abiotic stress tolerance in Chenopodium quinoa

Alshareef

Rey

Khoury

et al. 2019

Preprint

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Chenopodium quinoa Willd. (quinoa) is a pseudocereal with high nutritional value and relatively high tolerance to several abiotic stresses, including water deficiency and salt stress, making it a suitable plant for the study of mechanisms of abiotic stress tolerance. NAC (NAM, ATAF and CUC) transcription factors are involved in a range of plant developmental processes and in the response of plants to biotic and abiotic stresses. In the present study, we perform a genome-wide comprehensive analysis of the NAC transcription factor gene family in quinoa. In total, we identified 107 quinoa NAC transcription factor genes, distributed equally between sub-genomes A and B. They are phylogenetically clustered into two major groups and 18 subgroups. Almost 75% of the identified CqNAC genes were duplicated two to seven times and the remaining 25% of the CqNAC genes were found as a single copy. We analysed the transcriptional responses of the identified quinoa NAC TF genes in response to various abiotic stresses. The transcriptomic data revealed 28 stress responsive CqNAC genes, where their expression significantly changed in response to one or more abiotic stresses, including salt, water deficiency, heat and phosphate starvation. Among these stress responsive NACs, some were previously known to be stress responsive in other species, indicating their potentially conserved function in response to abiotic stress across plant species. Six genes were differentially expressed specifically in response to phosphate starvation but not to other stresses, and these genes may play a role in controlling plant responses to phosphate deficiency. These results provide insights into quinoa NACs that could be used in the future for genetic engineering or molecular breeding.

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Over-expression of a NAC 67 transcription factor from finger millet (Eleusine coracana L.) confers tolerance against salinity and drought stress in rice

Cited by 113 publications

References 59 publications

Role of DNA methylation dynamics in desiccation and salinity stress responses in rice cultivars

Role of DNA methylation dynamics in desiccation and salinity stress responses in rice cultivars

Knockout of the OsNAC006 Transcription Factor Causes Drought and Heat Sensitivity in Rice

Genome wide identification of NAC transcription factors and their role in abiotic stress tolerance in Chenopodium quinoa

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