Over- and Ectopic Expression of Wnt3 Causes Progressive Loss of Ameloblasts in Postnatal Mouse Incisor Teeth

Millar, Sarah E.; Koyama, Eiki; Reddy, Seshamma T.; Andl, Thomas; Gaddapara, T.; Piddington, Ronald; Gibson, Carolyn W.

doi:10.1080/03008200390152205

Cited by 47 publications

(24 citation statements)

References 20 publications

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“…Wnt3 is also expressed in the enamel knots. However, overexpression of Wnt3 by the K14 promoter in the epithelium did not result in significant change of tooth morphology [98]. Since mice deficient in many Wnt genes, including Wnt1, Wnt2, Wnt3, Wnt3a, Wnt4, and Wnt5a, either die too early for tooth phenotype analysis or do not exhibit a tooth phenotype, the detailed function for each Wnt member in tooth development is not clear at present [84,99,100].…”

Section: Wntsmentioning

confidence: 99%

Making a tooth: growth factors, transcription factors, and stem cells

et al. 2005

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Mammalian tooth development is largely dependent on sequential and reciprocal epithelial-mesenchymal interactions. These processes involve a series of inductive and permissive interactions that result in the determination, differentiation, and organization of odontogenic tissues. Multiple signaling molecules, including BMPs, FGFs, Shh, and Wnt proteins, have been implicated in mediating these tissue interactions. Transcription factors participate in epithelial-mesenchymal interactions via linking the signaling loops between tissue layers by responding to inductive signals and regulating the expression of other signaling molecules. Adult stem cells are highly plastic and multipotent. These cells including dental pulp stem cells and bone marrow stromal cells could be reprogrammed into odontogenic fate and participated in tooth formation. Recent progress in the studies of molecular basis of tooth development, adult stem cell biology, and regeneration will provide fundamental knowledge for the realization of human tooth regeneration in the near future.

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Section: Wntsmentioning

confidence: 99%

Making a tooth: growth factors, transcription factors, and stem cells

et al. 2005

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“…There is evidence that Wnt signaling and its modulation by Dkks is involved in bone formation (Kato et al, 2002;Rawadi et al, 2003;Akiyama et al, 2004;MacDonald et al, 2004). In addition, overexpression of Wnt3 in dental epithelium results in the loss of ameloblasts and a reduction of enamel in postnatal incisors, suggesting a role for the Wnt pathway in enamel formation (Millar et al, 2003). Thus, Dkks may be involved in differentiation of the amelo-and odontoblast cell lineages and subsequent dental hard tissue formation.…”

Section: Possible Roles For Dkks In Crown Calcificationmentioning

confidence: 99%

Dynamic expression of Wnt signaling-related Dickkopf1, -2, and -3 mRNAs in the developing mouse tooth

et al. 2005

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Wnt signaling is essential for tooth formation. Members of the Dickkopf (Dkk) family modulate the Wnt signaling pathway by binding to the Wnt receptor complex. Comparison of Dkk1, -2, and -3 mRNA expression during mouse tooth formation revealed that all three genes showed distinct spatiotemporally regulated expression patterns. Dkk1 was prominently expressed in the distal, incisor-bearing mesenchyme area of the mandibular process during the initial stages of tooth formation. During molar morphogenesis Dkk1 was detected in the dental mesenchyme, including the preodontoblasts. Dkk2 was seen in the dental papilla, whereas Dkk3 was specifically expressed in the putative epithelial signaling centers, the primary and secondary enamel knots. Postnatally, Dkk1 was prominently expressed in the preodonto-and odontoblasts, while Dkk3 mRNAs were transiently seen in the preameloblasts before the onset of enamel matrix secretion. These results suggest that modulation of Wnt-signaling by Dkks may serve important functions in patterning of dentition as well as in crown morphogenesis and dental hard-tissue formation. Developmental Dynamics 233:161-166, 2005.

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“…최근 들어 치아형성에 관여하는 이들 세포들의 분화유도인자와 독특한 세포-특이 적 유전자들에 대한 관심이 높아지고 있으나, 법랑모세포의 분화과정이나 법랑질의 형성과정의 분자생물학적 기전에 대하여 명확히 밝혀져 있지 않다 [24][25][26] . 상피기원인 법랑모세포는 법랑질 형성 각 시기의 주요활 성이 반영된 표현형의 변화를 점진적으로 보이는 독특한 생 활주기를 갖는다.…”

Section: Rt-pcrunclassified

A study of APin-protein interactions using protein microarray

Park

Kim

et al. 2007

J Korean Acad Conserv Dent

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Protein microarray or protein chips is potentially powerful tools for analysis of protein-protein interactions. APin cDNA was previously identified and cloned from a rat odontoblast cDNA library. The purpose of this study was to investigate the APin-protein interactions during ameloblast differentiation. Protein microarray was carried with recombinant APin protein and MEF2, Aurora kinase A, BMPR-IB and EFhand calcium binding protein were selected among 74 interacting proteins. Immortalized ameloblast cells (ALCs) were transfected with pCMV-APin construct and U6-APin siRNA construct. After transfection, the expression of the mRNAs for four proteins selected by protein micoarrays were assessed by RT-PCR.The results were as follows: 1. APin expression was increased and decreased markedly after its over-expression and inactivation, respectively. 2. Over-expression of the APin in the ALCs markedly down-regulated the expression of MEF2 and Aurora kinase A, whereas their expression remained unchanged by its inactivation. 3. Expression of BMPR-IB and EF-hand calcium binding protein were markedly increased by the overexpression of the APin in the ALCs, whereas expression of BMPR-IB remained unchanged and expression of EF-hand calcium binding protein was markedly decreased by its inactivation. These results suggest that APin plays an important role in ameloblast differentiation and mineralization by regulating the expression of MEF2, Aurora kinase A, BMPR-IB and EF-hand calcium binding protein.[J Kor Acad Cons Dent 32(5): [459][460][461][462][463][464][465][466][467][468] 2007]

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Over- and Ectopic Expression of Wnt3 Causes Progressive Loss of Ameloblasts in Postnatal Mouse Incisor Teeth

Cited by 47 publications

References 20 publications

Making a tooth: growth factors, transcription factors, and stem cells

Making a tooth: growth factors, transcription factors, and stem cells

Dynamic expression of Wnt signaling-related Dickkopf1, -2, and -3 mRNAs in the developing mouse tooth

A study of APin-protein interactions using protein microarray

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