Ovary transcriptome profiling of Coilia nasus during spawning migration stages by Illumina sequencing

Duan, Jinrong; Zhou, Yanfeng; Xu, Donghui; Zhang, Minying; She, Ying; Wei, Qiwei; Fang, Di‐An

doi:10.1016/j.margen.2015.02.005

Cited by 19 publications

(9 citation statements)

References 11 publications

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“…3b). In our previous transcriptome analysis, DnaJ homologs were the most abundant chaperone at the mRNA level in the normal ovary [17]. During C. nasus spawning migration process, fish will encounter lots of stress especially such as water temperature change and rising tide.…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies on ovary transcriptomic analysis in C. nasus have shown that DnaJs were up-regulated significantly during spawning migration [17]. Based on this bias, the full length cDNA of Cn-DnaJa1and DnaJb1 was cloned, tissues depended and temporal mRNA expression patterns in ovaries during different spawning migration phases were investigated; and finally Cn-DnaJa1and DnaJb1 protein during different development phases were clarified.…”

Section: Introductionmentioning

confidence: 99%

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Molecular cloning and characteristics of DnaJa1and DnaJb1 in Coilia nasus: possible function involved in oogenesis during spawning migration

et al. 2019

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Background Coilia nasus oogenesis/spawning migration is a well-defined synchronous arrangement process. DnaJs are indispensable molecular chaperones for oogenesis process. However, how DnaJs involved the anadromous spawning migration mechanism is outstanding and plausible. Results In this regard, two DnaJs (Cn-DnaJa1 and Cn-DnaJb1) are cloned from the Coilia nasus’ s ovary. Their structure both contains J domain, G/F domain and ZF domain. Their mRNA transcripts were found extensively expressed in all the sampled tissues and significantly highly in gonads, which probably mean that DnaJs involved in C. nasus’ s gonad development basal metabolic processes. In the process of spawning migration, Cn-DnaJa1 and Cn-DnaJb1 mRNA transcripts were also expressed with significant differences during oogenesis with highest levels in the development phase, and maintaining high levels during the multiplication, mature and spawning phase. Further study showed that the DnaJa1and DnaJb1protein have high distribution in the onset phase and mainly distributed in the oocyte cytoplasm especially during the migration development phase’s. Conclusions This experiment study demonstrated that DnaJs participate in reproductive regulation during the spawning migration process in C. nasus and possibly play a vital role in the ovary development process. These findings also provided a base knowledge for further molecular mechanism study of spawning migration.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Molecular cloning and characteristics of DnaJa1and DnaJb1 in Coilia nasus: possible function involved in oogenesis during spawning migration

et al. 2019

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“…The Japanese grenadier anchovy Coilia nasus , an ocean-river anadromous fish with spawning migration behavior, lives in the Yangtze River, coastal waters of China and Korean peninsula, and also in the Ariake Bay in Japan (Whitehead et al 1998 ; Zhu et al 2014 ; Du et al 2014 ; Shen et al 2015 ; Duan et al 2015 ; Zhou et al 2015 ). At spawning time every spring, mature C. nasus individuals migrate over long distances from ocean to freshwater lakes to spawn (Whitehead et al 1998 ; Zhu et al 2014 ).…”

Section: Introductionmentioning

confidence: 99%

Identification of olfactory receptor genes in the Japanese grenadier anchovy Coilia nasus

et al. 2017

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Olfaction is essential for fish to detect odorant elements in the environment and plays a critical role in navigating, locating food and detecting predators. Olfactory function is produced by the olfactory transduction pathway and is activated by olfactory receptors (ORs) through the binding of odorant elements. Recently, four types of olfactory receptors have been identified in vertebrate olfactory epithelium, including main odorant receptors (MORs), vomeronasal type receptors (VRs), trace-amine associated receptors (TAARs) and formyl peptide receptors (FPRs). It has been hypothesized that migratory fish, which have the ability to perform spawning migration, use olfactory cues to return to natal rivers. Therefore, obtaining OR genes from migratory fish will provide a resource for the study of molecular mechanisms that underlie fish spawning migration behaviors. Previous studies of OR genes have mainly focused on genomic data, however little information has been gained at the transcript level. In this study, we identified the OR genes of an economically important commercial fish Coilia nasus through searching for olfactory epithelium transcriptomes. A total of 142 candidate MOR, 52 V2R/OlfC, 32 TAAR and two FPR putative genes were identified. In addition, through genomic analysis we identified several MOR genes containing introns, which is unusual for vertebrate MOR genes. The transcriptome-scale mining strategy proved to be fruitful in identifying large sets of OR genes from species whose genome information is unavailable. Our findings lay the foundation for further research into the possible molecular mechanisms underlying the spawning migration behavior in C. nasus.Electronic supplementary materialThe online version of this article (doi:10.1007/s13258-017-0517-8) contains supplementary material, which is available to authorized users.

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“…It lays eggs from March to July, breeding once a year [24]. C. nasus provides a classic case-study for yearly spawning behavior, with migration distances that reach thousands of miles [20, 25]. …”

Section: Introductionmentioning

confidence: 99%

Changes of gonadotropin-releasing hormone receptor 2 during the anadromous spawning migration in Coilia nasus

et al. 2016

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BackgroundAn increase in the activity of the pituitary-gonad axis (PG-axis) and gonad development are essential for the onset of spawning migration in teleosts. In the fish Coilia nasus, gonad development and spawning migration up the Yangtze River occurs by the end of each summer. We hypothesized that gonadotropin releasing hormones receptor 2 (GnRH-R2), which together produce a signal that interacts with the PG-axis, may help to regulate spawning migration processes.ResultsIn this regard, we (1) characterized the gonadosomatic index (GSI) in the anadromous fish C. nasus; (2) analyzed the GnRH-R2 mRNA expression levels in ovary and brain, and concentrations in the serum; and (3) identified the GnRH-R2 protein distribution in the brain and ovaries. We found strong relationships between all of these indices.ConclusionsThe results indicate that GnRH-R2 could act together to promote spawning during the anadromous migration. There is some evidence that the GnRH-R2 gene expression levels and protein distributions change in association with the migratory behavior.

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Ovary transcriptome profiling of Coilia nasus during spawning migration stages by Illumina sequencing

Cited by 19 publications

References 11 publications

Molecular cloning and characteristics of DnaJa1and DnaJb1 in Coilia nasus: possible function involved in oogenesis during spawning migration

Molecular cloning and characteristics of DnaJa1and DnaJb1 in Coilia nasus: possible function involved in oogenesis during spawning migration

Identification of olfactory receptor genes in the Japanese grenadier anchovy Coilia nasus

Changes of gonadotropin-releasing hormone receptor 2 during the anadromous spawning migration in Coilia nasus

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