Ovariectomy induces oxidative stress and impairs bone antioxidant system in adult rats

Muthusami, Sridhar; Ilangovan, R.; Balaganesh, Muthusamy; Gopalakrishnan, V. K.; Venkataraman, P.; Subramaniam, Veni; Arunakaran, J.; Srinivasan, Narasimhan

doi:10.1016/j.cccn.2005.04.014

Cited by 276 publications

(200 citation statements)

References 36 publications

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“…The estrogen deficiency state itself may impair the bone anti-oxidant system and lowered the SOD and GPX activities of ovariectomized rats. 48 Intake of antioxidants such as vitamin E helps the internal antioxidant enzymes to overcome oxidative stress. 49 a-Tocopherol may have supported SOD by providing external anti-oxidant defense and therefore preventing the reduction of the SOD activity as seen in our study.…”

Section: Discussionmentioning

confidence: 99%

Effects of α‐tocopherol on the early phase of osteoporotic fracture healing

Shuid

Mohamad

Muhammad

et al. 2011

Journal Orthopaedic Research

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Fracture healing is a complex process, which is more complicated if the bone is osteoporotic. One of the vitamin E isomers, a-tocopherol, has been found to prevent osteoporosis and improve bone fracture healing but its role in the healing of osteoporotic fractures is still unclear. We carried out a study on the effects of a-tocopherol supplementation on osteoporotic fracture healing using an ovariectomized rat model, whereby we focused on the early phase of fracture healing, that is, the phase with excessive production of free radicals. Twenty-four female Sprague-Dawley rats were divided into three groups: sham-operated (SO), ovariectomized-control (OVC), and ovariectomized þ a-tocopherol supplementation (ATF) groups. The right femora of all the rats were fractured at middiaphysis and K-wires were inserted for internal fixation. After 2 weeks of treatment, the rats were euthanized and the femora were dissected out for measurement of callous volume by CT-scan and radiological staging of callous formation and fracture healing. The oxidative parameters of the fractured femora were also measured. The results showed that the callous volume and callous staging were not different between the groups. However, the fracture healing stage of the OVC group was lower than the SO group, while atocopherol supplementation in the ATF group had improved the healing until it was comparable to the SO group. The activities of the anti-oxidatant enzymes, superoxide dismutase, and glutathione peroxidase in the ATF group were found to be significantly higher than in the OVC group. In conclusion, a-tocopherol improved fracture healing but had no effect on the callous volume and staging. The improvement in fracture healing may be due to the increased activities of the anti-oxidatant enzymes in the bone during the early phase of fracture healing of osteoporotic bone. ß

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Section: Discussionmentioning

confidence: 99%

Effects of α‐tocopherol on the early phase of osteoporotic fracture healing

Shuid

Mohamad

Muhammad

et al. 2011

Journal Orthopaedic Research

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“…The most upstream effects of ovx in the BM are to stimulate the production of reactive oxygen species (ROS) and to impair the generation of antioxidants (27,37,50,51). In response to ovx, ROS are produced by most BM cells including T cells (52).…”

Section: T Cells Mediate the Effects Of Estrogen Deficiency In Bonementioning

confidence: 99%

Osteoimmunology and Its Implications for Transplantation

Pacifici

2013

American Journal of Transplantation

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Osteoimmunology is a field of research dedicated to the study of the interactions between the immune system, the hemopoietic system and bone. Among the cells of the immune system that regulate bone cells and the hemopoietic function are T lymphocytes. These cells secrete inflammatory cytokines that promote bone resorption, as well as Wnt ligands that stimulate bone formation. In addition, T cells regulate bone homeostasis by cross talking with BM stromal cells and osteoblastic cells via CD40 ligand (CD40L) and other costimulatory molecules. This article describes the immune cells relevant to bone and the hemopoietic function, reviews the role of lymphocytes as mediators of the effects of PTH and estrogen in bone and the hemopoietic system and discusses the implication of osteoimmunology for transplant medicine.

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“…Over the last decade, evidence has accumulated of ROS participation in bone resorption, with a direct contribution of osteoclasts generating high concentrations of superoxide anion (O2-) and hydrogen peroxide (H2O2) (Muthusami et al, 2005;Bai et al, 2006). However, the mechanisms by which ROS participate in accelerating the destruction of calcified tissue and hence bone resorption have not been fully explained (Muthusami et al, 2005;Ding et al, 2006). Moreover, the effect of ROS on osteoblastic function remains unclear (Ding et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

“…In addition, several risk factors for osteoporosis, such as diabetes mellitus (Ding et al, 2006;Léger et al, 2006) and estrogen deficiency (Godsland, 2005;Muthusami et al, 2005) are associated with increased oxidative stress. In diabetes mellitus, persistent hyperglycemia may cause oxidative stress induced by a variety of mechanisms including glucose autoxidation and accelerated glycation reaction, with the formation of glucose-derived advanced glycosylation end products (AGEs), which in turn catalyze lipid peroxidation (Valko et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Relationship between oxidative stress and diabetic osteopenia in premenopausal rats

Duarte

Rodrigues

Rezende

et al. 2010

Braz. J. Pharm. Sci.

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The relationship between lipid peroxidation, antioxidant defense and diabetic osteopenia remains unclear. This study evaluated the relationship among lipid peroxidation index, antioxidant defense parameters and bone metabolism in a premenopausal diabetic model using measures including thiobarbituric acidreactive substances concentration (TBARS) and reduced glutathione (GSH) content in brain homogenates, histomorphometric analysis, biomechanical testing and bone mineral density (BMD). Female Wistar rats with regular estrous cycle were divided into two groups: Group 1: control rats (n = 15) and Group 2: diabetic rats (n = 15). Diabetes was induced by alloxan and confirmed by glycemia ≥250 mg/dL. The lipid peroxidation index, measured by TBARS concentration, showed a significant increase (p<0.05) in diabetic animals in comparison to control animals. However, the antioxidant parameter measured by GSH content, was significantly lower (p<0.05) in diabetic animals. Histomorphometric analysis showed a significant increase (p<0.05) in femoral trabecular separation together with a significant decrease (p<0.05) in trabecular thickness, and reduced trabecular bone volume in diabetic rats. Moreover, biomechanical testing and BMD values were significantly lower (p<0.05) in the diabetic group. Thus, our results demonstrated that increased lipid peroxidation and altered antioxidant defense could be related to the development of oxidative stress and diabetic osteopenia in premenopausal rats.Uniterms: Oxidative stress. Lipid peroxidation. Diabetes mellitus. Diabetic osteopenia/experimental study.A relação entre peroxidação lipídica, defesa antioxidante e osteopenia diabética permanece obscura. Este estudo avaliou a associação entre índice de peroxidação lipídica, parâmetro de defesa antioxidante e metabolismo ósseo em um modelo diabético pré-menopausa através de medidas como a concentração de substâncias reativas ao ácido tiobarbitúrico (SRAT) e conteúdo de glutationa reduzida (GSH) no homogenato cerebral, análises histomorfométricas, teste biomecânico e densidade mineral óssea (DMO). Ratos Wistar fêmeas com ciclo estral regular foram distribuídos em dois grupos: Grupo 1 -ratas controle (n = 15) e Grupo 2 -ratas diabéticas (n = 15). O diabetes foi induzido pela aloxana e confirmado pela glicemia ≥250 mg/dL. O índice de peroxidação lipídica, medido pela concentração de SRAT, demonstrou um aumento significativo (p<0.05) nos animais diabéticos, em relação aos animais controle. Entretanto, o parâmetro de defesa antioxidante, mensurado pelo conteúdo de GSH, foi reduzido significativamente (p<0.05) nos animais diabéticos. As análises histomorfométricas mostraram um aumento significativo (p<0.05) da separação trabecular do fêmur, associado à diminuição significativa da espessura trabecular (p<0.05) e volume ósseo trabecular reduzido nas ratas diabéticas. Além disso, o teste biomecânico, V.M.G. Duarte, A. de S. Rodrigues, L.A. de Rezende, A.Ma. de O. Ramos, R.M. de Souza, F.P.F. Neto, A. da C. Medeiros 540 medido pela força máxima, e val...

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Ovariectomy induces oxidative stress and impairs bone antioxidant system in adult rats

Cited by 276 publications

References 36 publications

Effects of α‐tocopherol on the early phase of osteoporotic fracture healing

Effects of α‐tocopherol on the early phase of osteoporotic fracture healing

Osteoimmunology and Its Implications for Transplantation

Relationship between oxidative stress and diabetic osteopenia in premenopausal rats

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