Ovariectomy Increases the Participation of Hyperpolarizing Mechanisms in the Relaxation of Rat Aorta

Sagredo, Ana; Campo, Lara del; Martorell, Aina; Navarro, Rocío; Martín, Maria Carmen; Blanco-Rivero, Javier; Ferrer, Mercedes

doi:10.1371/journal.pone.0073474

Cited by 13 publications

(12 citation statements)

References 43 publications

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“…This points toward alterations of the vasodilator effect of cGMP-dependent protein kinase (PKG) signaling cascade in NP 6.5 and Cu 0 rats. The observed results might be explained by the fact that soluble guanylate cyclase (sGC) activity and cGMP- dependent protein kinase expression have been previously shown to be negatively regulated by proinflammatory mediators [33,34].…”

Section: Discussionmentioning

confidence: 99%

Dietary supplementation with copper nanoparticles influences the markers of oxidative stress and modulates vasodilation of thoracic arteries in young Wistar rats

et al. 2020

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We aimed to study the physiological effects of diet supplemented with copper (Cu) nanoparticles (NPs). During the eight weeks of the experiment, young Wistar rats (at seven weeks of age, n = 9) were supplemented with 6.5 mg of Cu either as NPs or carbonate salt (Cu 6.5). A diet that was not supplemented with Cu served as a negative control (Cu 0). The impact of nano Cu supplementation on lipid (reflected as thiobarbituric acid reactive substances-TBARS) and protein peroxidation (thiol and carbonyl groups) in blood plasma as well as the influence on the vasodilatory mechanism(s) of isolated rat thoracic arteries were studied. Supplementation with Cu enhanced lipid peroxidation (TBARS) in NP 6.5 (x2.4) and in Cu 6.5 (x1.9) compared to the negative control. Significant increase in TBARS was also observed in NP 6.5 (x1.3) compared to the Cu 6.5 group. The level of thiol groups increased in NP 6.5 (x1.6) compared to Cu 6.5. Meanwhile, significant (x0.6) decrease was observed in the Cu 6.5 group compared to the negative control. Another marker of protein oxidation, carbonyl groups increased in NP 6.5 (x1.4) and Cu 6.5 (x2.3) compared to the negative control. However significant difference (x0.6) was observed between NP 6.5 and Cu 6.5. Arteries from Cu supplemented rats exhibited an enhanced vasodilation to gasotransmitters: nitric oxide (NO) and carbon monoxide (CO). An enhanced vasodilation to NO was reflected in the increased response to acetylcholine (ACh) and calcium ionophore A23187. The observed responses to ACh and CO releasing molecule (CORM-2) were more pronounced in NP 6.5. The activator of cGMP-dependent protein kinases (8-bromo-cGMP) induced similar vasodilation of thoracic arteries in NP 6.5 and Cu 0 groups, while an increased response was observed in the Cu 6.5 group. Preincubation with the inducible nitric oxide (iNOS) synthase inhibitor-1400W, decreased the ACh-induced vasodilation in NP 6.5 , exclusively. Meanwhile the eicosanoid metabolite of arachidonic acid (20-HETE) synthesis inhibitor-HET0016, enhanced vasodilation of arteries from Cu 0 group. In conclusion, this study demonstrates that supplementation with nano Cu influences oxidative stress, which further has modified the vascular response.

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Section: Discussionmentioning

confidence: 99%

Dietary supplementation with copper nanoparticles influences the markers of oxidative stress and modulates vasodilation of thoracic arteries in young Wistar rats

et al. 2020

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“…The fluorescent probe, hydroethidine, was used to evaluate superoxide anion levels in situ , as previously described [13, 26]. Mesenteric segments from the three groups were cryoprotected with 30% (w/v) sucrose in PBS, frozen and stored at -80°C.…”

Section: Methodsmentioning

confidence: 99%

“…The method used for isometric tension recording has been previously described [26, 27]. In summary, mesenteric artery segments were suspended in an organ bath containing 5 mL of KHS at 37°C, continuously bubbled with 95% O 2 -5% CO 2 mixtures (pH 7.4).…”

Section: Methodsmentioning

confidence: 99%

Docosahexaenoic Acid Supplemented Diet Influences the Orchidectomy-Induced Vascular Dysfunction in Rat Mesenteric Arteries

et al. 2017

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Over the past few decades, the cardiovascular benefits of a high dietary intake of long-chain polyunsaturated fatty acids (PUFAs), like docosahexaenoic acid (DHA), have been extensively studied. However, many of the molecular mechanisms and effects exerted by PUFAs have yet to be well explained. The lack of sex hormones alters vascular tone, and we have described that a DHA-supplemented diet to orchidectomized rats improve vascular function of the aorta. Based on these data and since the mesenteric artery importantly controls the systemic vascular resistance, the objective of this study was to analyze the effect of a DHA-supplemented diet on the mesenteric vascular function from orchidectomized rats. For this purpose mesenteric artery segments obtained from control, orchidectomized or orchidectomized plus DHA-supplemented diet were utilized to analyze: (1) the release of prostanoids, (2) formation of NO and ROS, (3) the vasodilator response to acetylcholine (ACh), as well as the involvement of prostanoids and NO in this response, and (4) the vasoconstrictor response to electrical field stimulation (EFS), analyzing also the effect of exogenous noradrenaline (NA), and the NO donor, sodium nitroprusside (SNP). The results demonstrate beneficial effects of DHA on the vascular function in orchidectomized rats, which include a decrease in the prostanoids release and superoxide formation that were previously augmented by orchidectomy. Additionally, there was an increase in endothelial NO formation and the response to ACh, in which NO involvement and the participation of vasodilator prostanoids were increased. DHA also reversed the decrease in EFS-induced response caused by orchidectomy. All of these findings suggest beneficial effects of DHA on vascular function by reversing the neurogenic response and the endothelial dysfunction caused by orchidectomy.

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“…On the other hand, it is well established that sex hormones regulate vascular function by altering the release and cell signaling pathways of endothelial factors that could lead to vascular dysfunction. In this regard, we have demonstrated that the loss of gonadal function modifies the production of vasoconstrictor prostanoids [ 21 – 24 ], superoxide anion [ 25 – 27 ] and NO [ 28 ]. Since the number of vascular pathologies matching with decreased levels of sex hormones is increasing [ 29 , 30 ], it would be of interest investigate whether a DHA-supplemented diet could prevent vascular changes induced when an impaired gonadal function exist (i.e.…”

Section: Introductionmentioning

confidence: 99%

Effect of Dietary Docosahexaenoic Acid Supplementation on the Participation of Vasodilator Factors in Aorta from Orchidectomized Rats

et al. 2015

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Benefits of n-3 polyunsaturated fatty acids (PUFAs) against cardiovascular diseases have been reported. Vascular tone regulation is largely mediated by endothelial factors whose release is modulated by sex hormones. Since the incidence of cardiovascular pathologies has been correlated with decreased levels of sex hormones, the aim of this study was to analyze whether a diet supplemented with the specific PUFA docosahexaenoic acid (DHA) could prevent vascular changes induced by an impaired gonadal function. For this purpose, control and orchidectomized rats were fed with a standard diet supplemented with 5% (w/w) sunflower oil or with 3% (w/w) sunflower oil plus 2% (w/w) DHA. The lipid profile, the blood pressure, the production of prostanoids and nitric oxide (NO), and the redox status of biological samples from control and orchidectomized rats, fed control or DHA-supplemented diet, were analyzed. The vasodilator response and the contribution of NO, prostanoids and hyperpolarizing mechanisms were also studied. The results showed that orchidectomy negatively affected the lipid profile, increased the production of prostanoids and reactive oxygen species (ROS), and decreased NO production and the antioxidant capacity, as well as the participation of hyperpolarizing mechanisms in the vasodilator responses. The DHA-supplemented diet of the orchidectomized rats decreased the release of prostanoids and ROS, while increasing NO production and the antioxidant capacity, and it also improved the lipid profile. Additionally, it restored the participation of hyperpolarizing mechanisms by activating potassium. Since the modifications induced by the DHA-supplemented diet were observed in the orchidectomized, but not in the healthy group, DHA seems to exert cardioprotective effects in physiopathological situations in which vascular dysfunction exists.

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Ovariectomy Increases the Participation of Hyperpolarizing Mechanisms in the Relaxation of Rat Aorta

Cited by 13 publications

References 43 publications

Dietary supplementation with copper nanoparticles influences the markers of oxidative stress and modulates vasodilation of thoracic arteries in young Wistar rats

Dietary supplementation with copper nanoparticles influences the markers of oxidative stress and modulates vasodilation of thoracic arteries in young Wistar rats

Docosahexaenoic Acid Supplemented Diet Influences the Orchidectomy-Induced Vascular Dysfunction in Rat Mesenteric Arteries

Effect of Dietary Docosahexaenoic Acid Supplementation on the Participation of Vasodilator Factors in Aorta from Orchidectomized Rats

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