Ovariectomy differential influence on some hemostatic markers of mice and rats

Abstract

Rodent ovariectomy is an experimental method to eliminate the main source of sexual steroids. This work explored for the first time the ovariectomy temporal changes induced in the hemostatic coagulation markers: prothrombin time (PT), activated partial thromboplastin time (aPTT), thrombin time (TT), and fibrinogen concentration (FIB) along with uterine weight on adult female CD1 mice and Wistar rats. Uterine weight (Uw) was assessed before ovariectomy (control), and 1, 3, 5, 7, 9, 16, and 21 days after surgery. PT, aPTT, TT and FIB were estimated the same days, using reported standard techniques. Ovariectomy decreased Uw, since day 1; and from day 10 to 21 reached the lowest values for both species. After day 1, mice hemostatic parameters changed (PT +10%, P<0.05; aPTT +53%, P<0.05; TT −24%, P<0.05; FIB +67%, P<0.05). Rats showed significant changes only in TT and FIB (TT −13%, P<0.001; FIB +65%, P<0.001). Neither mice PT, aPTT and TT, recovered control values after 21 days. In the rats from day 5 to 16 aPTT diminished (18–23%, P<0.05) recovering to control values on day 21, TT after 9 days and PT on day 16. In both species, FIB returned to its control values after 9 days. Ovariectomy differentially altered the PT hemostatic parameter of mice and rats indicating a non-equivalence among both species behaviour for experimental studies of blood coagulation.

The influence of female sex hormones on lung inflammation after brain death ‐ an experimental study

“…In this context, it is important to consider that, unlike in non-OVx rats, the hormonal reduction in OVx group occurred over a period of 10 days until the BD induction. Evidence has shown that after ovariectomy, there is a gradual decrease in uterine weight until the ninth day, indicating an adaption period [15]. In parallel, we also observed a reduction of corticosterone levels after OVx, characterizing an alteration of the hormonal milieu during this time.…”

Unexpected Role of Physiological Estrogen in Acute Stress-Induced Memory Deficits

“…2 E ). Seeking a second, independent biological marker of estradiol levels, we determined uterine weights, which have been shown to fluctuate across the estrous cycle and depend on systemic estrogen levels ( Balmain et al, 1956 ; Galloa et al, 1986 ; Lemini et al, 2015 ). Uterine indices (uterus weight (g)/body weight (g) × 100) of mice entering proestrus were greater than those in estrus ( t (23) = 7.52, p < 0.0001; Fig.…”

Maca extracts and estrogen replacement therapy in ovariectomized rats exposed at high altitude

“…On the contrary, the estradiol replacement countered the decrease in uterine weight in both groups of OVX rats exposed to normoxia and hypoxia. Drop of uterine weight is associated with increase in LH levels and decrease of estrogen in plasma 44 . The treatment with estradiol of OVX rats increases the proliferation in the uterine endometrium or uterotrophic activity, and this could be mediated by an upregulation of estrogen receptors alpha (ERα) than beta (ERβ) numbers 45 .…”