Ovarian tissue cryopreservation followed by controlled ovarian stimulation and pick-up of mature oocytes does not impair the number or quality of retrieved oocytes

Purpose The aim of this study was to evaluate long-term embryo cryopreservation, utilization, and success rate in patients subjected to gonadotoxic treatments in the context of cancer. Methods This is a retrospective study on patients (n=54) undergoing ovarian stimulation and IVF for fertility preservation between January 1997 and June 2014. Embryos were slowfrozen and stored until the women were cured and able to undergo embryo transfer. Results Fifty-four women underwent 66 oocyte pick-up procedures in total, and embryos were obtained from 52 of the 54 patients. Four patients died before their frozen embryos could be thawed. Of the remaining 48, 9 women returned to use their embryos, resulting in 6 pregnancies (66 % cumulative pregnancy rate), two of which ended in miscarriage. The live birth rate per patient was thus 44 % (4/9). The true come-back rate, calculated after applicable exclusions, was found to be 23 %. Conclusion IVF followed by embryo freezing is a widely established technique for fertility preservation, but little has been published on the outcomes in cancer patients. While we found the number of good-quality embryos to be lower than in a normal population, the cumulative live birth rate was similar to that achieved with fresh embryos in non-cancer patients. The utilization rate of this fertility preservation method can be considered high.

Section: Number Of Oocytes and Embryossupporting

confidence: 88%

Utilization rates and results of long-term embryo cryopreservation before gonadotoxic treatment

Dolmans

Ouderaen

Demylle

et al. 2015

“…Minimal stimulation with gonadotropins before ovariectomy might stimulate follicles to grow and more competent oocytes could potentially be obtained. COS followed by hCG maturation trigger and oocyte retrieval 34-36 h later, combined with ovarian tissue biopsy after oocyte retrieval has been described [46], however with a negative impact on the quality of ovarian tissue for cryopreservation [47].…”

Section: Discussionmentioning

confidence: 99%

In vitro maturation (IVM) of oocytes recovered from ovariectomy specimens in the laboratory: a promising “ex vivo” method of oocyte cryopreservation resulting in the first report of an ongoing pregnancy in Europe

Segers¹,

Mateizel²,

Moer³

et al. 2015

126

105

“…It was indeed recently demonstrated that the number of oocytes obtained after COS, following immediately OTC, was similar to a control group of the same age [14].…”

Section: Live Birthmentioning

confidence: 99%

Ovarian cortex transplantation: 60 reported live births brings the success and worldwide expansion of the technique towards routine clinical practice

Donnez

Dolmans

2015

Self Cite

302

201