In vitro maturation (IVM) of oocytes recovered from ovariectomy specimens in the laboratory: a promising “ex vivo” method of oocyte cryopreservation resulting in the first report of an ongoing pregnancy in Europe

Segers, Ingrid; Mateizel, Ileana; Moer, Ellen Van; Smitz, Johan; Tournaye, Herman; Verheyen, Greta; Vos, M. De

doi:10.1007/s10815-015-0528-9

Cited by 128 publications

(114 citation statements)

References 49 publications

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“…In one patient all three oocytes collected were degenerated at collection, while in another patient three of five oocytes matured during IVM. A low MII rate was reported in prepubertal girls in previous study [7,10], but the collective experience with IVM of oocytes from prepubertal girls does not allow any conclusions to be drawn and should be expanded on in future studies.…”

Section: Discussionmentioning

confidence: 83%

“…A number of studies have shown that immature oocytes can be aspirated from small antral follicles visible on the surface of the ovary after excision usually with diameters exceeding 3 millimeter [4,5]. Two pregnancies resulting from cryopreserved embryos obtained from in vitro maturation (IVM) oocytes have recently been reported from women who recovered from cancer [6,7].…”

Section: Introductionmentioning

confidence: 99%

“…Small antral follicles collected from surplus medulla tissue are present in relatively large number and the oocytes may represent a potentially valuable source for fertility preservation, providing that they undergo proper IVM. However, the maturation rate of immature oocytes collected at the time of ovarian cortex cryopreservation ranged from 3 % to 100 % [7][8][9][10][11]. Thus, more data are needed to estimate the overall success rate and safety of this method.…”

Section: Introductionmentioning

confidence: 99%

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Vitrification of in vitro matured oocytes collected from surplus ovarian medulla tissue resulting from fertility preservation of ovarian cortex tissue

Yin

Jiang

Kristensen

et al. 2016

J Assist Reprod Genet

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Purpose The aim of the study was to investigate the maturation rate of immature oocytes collected from ovarian medulla tissue normally discarded during preparation of ovarian cortical tissue for fertility preservation. Further we evaluated survival of derived MII oocytes following vitrification and warming. Methods 36 patients aged from 8 to 41 years who had one ovary excised for fertility preservation were included. Oocytes were collected from the medulla tissue and matured in vitro 44-48 h followed by vitrification. Number of oocytes collected, the rates of maturation and post-warming survival were assessed. Results On average, 11 immature oocytes were collected per patient. The overall maturation rate was 29 % irrespective of whether the ovary was transported 4-5 h on ice or obtained immediately after oophorectomy. The maturation rate in patients below 20 years of age (55 %) was significantly higher than that of patients aged 20-30 years (29 %) and above 30 years (26 %). The post-warming survival rate was 64 %. No significant relationship was observed between the number of collected oocytes and the age of patients.Conclusions Approximately three MII oocytes were obtained per patient following in vitro maturation (IVM) of immature oocytes collected from medulla tissue, of which two survived vitrification and warming. This approach represents an add-on method to potentially augment the fertility opportunity for cancer patients, especially in young women with cancer where transplantation of cortical tissue may pose a risk of relapse, but the IVM approach is currently too inefficient to be the only method used for fertility preservation.

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Section: Discussionmentioning

confidence: 83%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Vitrification of in vitro matured oocytes collected from surplus ovarian medulla tissue resulting from fertility preservation of ovarian cortex tissue

Yin

Jiang

Kristensen

et al. 2016

J Assist Reprod Genet

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“…Mature eggs derived from IVM can be cryopreserved or fertilized and then cryopreserved as embryos. In vitro maturation (IVM) is another promising fertility preservation option, and importantly, pregnancies and live births have been obtained using this method (Chian, et al 2013, Guzman, et al 2012, Segers, et al 2015, Uzelac, et al 2015). …”

Section: Effect Of Ovarian Tissue Transport On Oocytes From Antral Fomentioning

confidence: 99%

Ovarian tissue transport to expand access to fertility preservation: from animals to clinical practice

et al. 2016

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Primordial follicles dictate a female’s reproductive lifespan and therefore are central to fertility preservation for both endangered species as well as for individuals with fertility threatening conditions. Ovarian tissue containing primordial follicles can be cryopreserved and later thawed and transplanted back into individuals to restore both endocrine function and fertility. Importantly, increasing numbers of human live births have been reported following ovarian tissue cryopreservation and transplantation. A current limitation of this technology is patient access to sites that are approved or equipped to process and cryopreserve ovarian tissue – especially in larger countries or low resource settings. Here we review empirical evidence from both animal models and human studies that suggest that ovarian tissue can be transported at cold temperatures for several hours while still maintaining the integrity and reproductive potential of the primordial follicles within the tissue. In fact, several human live births have been reported in European countries using tissue that was transported at cold temperatures for up to 20 hours prior to cryopreservation and transplantation. Ovarian tissue transport, if implemented widely in clinical practice, could therefore expand both patient and provider access to emerging fertility preservation options.

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“…There are currently a variety of methods of fertility preservation, including cryopreservation of ovarian tissue (Segers et al, 2015), mature oocytes (Walls et al, 2015), and embryos. For unmarried women with breast cancer or malignant blood disorders, it is not rare to choose the resection and cryopreservation of ovarian tissue.…”

Section: Introductionmentioning

confidence: 99%

In vitro maturation and cryopreservation of oocytes retrieved from intra-operative aspiration during second enucleation for ovarian tumor: A case report

Shirasawa

Kumazawa

Sato

et al. 2017

Gynecologic Oncology Reports

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In vitro maturation (IVM) of oocytes recovered from ovariectomy specimens in the laboratory: a promising “ex vivo” method of oocyte cryopreservation resulting in the first report of an ongoing pregnancy in Europe

Cited by 128 publications

References 49 publications

Vitrification of in vitro matured oocytes collected from surplus ovarian medulla tissue resulting from fertility preservation of ovarian cortex tissue

Vitrification of in vitro matured oocytes collected from surplus ovarian medulla tissue resulting from fertility preservation of ovarian cortex tissue

Ovarian tissue transport to expand access to fertility preservation: from animals to clinical practice

In vitro maturation and cryopreservation of oocytes retrieved from intra-operative aspiration during second enucleation for ovarian tumor: A case report

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