Ovarian gene expression in the absence of FIGLA, an oocyte-specific transcription factor

Joshi, Saurabh; Davies, Holly G.; Sims, Lauren; Levy, Shawn; Dean, Jurrien

doi:10.1186/1471-213x-7-67

Cited by 110 publications

(89 citation statements)

References 77 publications

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“…As shown in Fig. 3A, Sddr transcript was strongly expressed in ovary, which corresponds well with the recent report by Joshi et al (2007). In addition, RT-PCR analysis suggested that Sddr is expressed also in lung (Fig.…”

Section: Expression Of Sddr In Adult Tissuessupporting

confidence: 78%

A stem cell-derived gene (Sddr) negatively regulates differentiation of embryonic stem cells

Miura¹,

Ueda²,

Takao³

et al. 2010

Int. J. Dev. Biol.

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Embryonic stem (ES) cells, derived from the inner cell mass of blastocysts, are pluripotent and continue to self-renew. To better understand the molecular mechanisms underlying self-renewal, we have been searching for a gene(s) which is specifically expressed in selfrenewing ES cells. Here we report the isolation and characterization of a novel gene, Sddr (stem cell-derived differentiation regulator). Sddr was highly expressed in undifferentiated ES cells, and its expression was downregulated upon differentiation. In addition to ES cells, Sddr expression was observed strongly in ovary, and weakly in lung. Immunostaining and cellular fractionation analyses suggested that Sddr is a cytoplasmic protein associated with the cytoskeleton. Sddr-null ES cells showed no remarkable abnormalities in their undifferentiated state. In contrast, in differentiating Sddr-null cells, induction of several differentiation-associated markers was enhanced, and downregulation of self-renewal marker genes was accelerated, as compared with wild-type cells. These results suggest that although it is dispensable for ES cell self-renewal, Sddr is a negative regulator of ES cell differentiation.

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Section: Expression Of Sddr In Adult Tissuessupporting

confidence: 78%

A stem cell-derived gene (Sddr) negatively regulates differentiation of embryonic stem cells

Miura¹,

Ueda²,

Takao³

et al. 2010

Int. J. Dev. Biol.

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“…However, the more systematic transcriptome profiling of growing oocytes has begun to identify new candidate factors (Hamatani et al, 2004a;Evsikov et al, 2006). In particular, the categorization of downstream targets of oocytespecific transcription factors has provided a rich source of additional candidate maternal-effect genes (Joshi et al, 2007). More recently, the repertoire of maternal-effect genes has been further expanded to include multi-protein complexes that have crucial roles in mouse preimplantation development (Li et al, 2008a;Zheng and Dean, 2009).…”

Section: Introductionmentioning

confidence: 99%

Maternal control of early mouse development

2010

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The hiatus between oocyte and embryonic gene transcription dictates a role for stored maternal factors in early mammalian development. Encoded by maternal-effect genes, these factors accumulate during oogenesis and enable the activation of the embryonic genome, the subsequent cleavage stages of embryogenesis and the initial establishment of embryonic cell lineages. Recent studies in mice have yielded new findings on the role of maternally provided proteins and multi-component complexes in preimplantation development. Nevertheless, significant gaps remain in our mechanistic understanding of the networks that regulate early mammalian embryogenesis, which provide an impetus and opportunities for future investigations.

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“…Figla (factor in the germ line, alpha; official name, folliculogenesis-specific basic helix-loop-helix) encodes a germ cell-specific bHLH transcription factor that was initially identified by its ability to coordinate the expression of the oocyte-specific zona pellucida genes (28). Although Figla transcripts are first detected in oocytes at E14.5, few differences are observed between normal and null transcriptomes prior to birth (20), at which time null oocytes do not form primordial follicles, a defining structure in the female gonad, and become sterile. Low levels of FIGLA are detected in adult testes (28), but Figla null males have normal testicular histology and are fertile (46).…”

mentioning

confidence: 99%

FIGLA, a Basic Helix-Loop-Helix Transcription Factor, Balances Sexually Dimorphic Gene Expression in Postnatal Oocytes

Gauthier

Baibakov

et al. 2010

Molecular and Cellular Biology

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Maintenance of sex-specific germ cells requires balanced activation and repression of genetic hierarchies to ensure gender-appropriate development in mammals. Figla (factor in the germ line, alpha) encodes a germ cell-specific basic helix-loop-helix transcription factor first identified as an activator of oocyte genes. In comparing the ovarian proteome of normal and Figla null newborn mice, 18 testis-specific or -enhanced proteins were identified that were more abundant in Figla null ovaries than in normal ovaries. Transgenic mice, ectopically expressing Figla in male germ cells, downregulated a subset of these genes and demonstrated age-related sterility associated with impaired meiosis and germ cell apoptosis. Testis-associated genes, including Tdrd1, Tdrd6, and Tdrd7, were suppressed in the transgenic males with a corresponding disruption of the sperm chromatoid body and mislocalization of MVH and MILI proteins, previously implicated in posttranscriptional processing of RNA. These data demonstrate that physiological expression of Figla plays a critical dual role in activation of oocyte-associated genes and repression of sperm-associated genes during normal postnatal oogenesis.Mouse gestation takes ϳ20 days, and at embryonic day 10.5 (E10.5) the bipotential mammalian gonad commits to becoming a testis or an ovary, depending on the presence of the male-specifying Y chromosome. Subsequent gender-specific genetic hierarchies modulate the transition of the undifferentiated gonad to an ovary or testis, which produces haploid gametes through the reductive divisions of meiosis (5). However, sexual fate is plastic among metazoans (e.g., flies, worms, and mice), and this reversible commitment indicates an ongoing need for genetic controls to maintain one sex with concomitant nonexpression of genes that support the opposite identity (21, 33). Recently, Foxl2, a forkhead transcription factor expressed in somatic cells, has been implicated in maintaining female gonad sexual identify in adult mice; its ablation in postnatal ovaries leads to sex reversal via upregulation of Sox9 in somatic tissue independent of oocytes (51). It seems likely that genetic hierarchies expressed within oocytes complement these somatic signals to maintain appropriate germ cell identity by activating oocyte-associated genes and repressing sperm-associated genes during postnatal oogenesis.Members of the basic helix-loop-helix (bHLH) family of transcription factors are key regulators of cell growth and differentiation that can either activate or repress transcription of target genes (19). Figla (factor in the germ line, alpha; official name, folliculogenesis-specific basic helix-loop-helix) encodes a germ cell-specific bHLH transcription factor that was initially identified by its ability to coordinate the expression of the oocyte-specific zona pellucida genes (28). Although Figla transcripts are first detected in oocytes at E14.5, few differences are observed between normal and null transcriptomes prior to birth (20), at which time null oocytes do...

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Ovarian gene expression in the absence of FIGLA, an oocyte-specific transcription factor

Cited by 110 publications

References 77 publications

A stem cell-derived gene (Sddr) negatively regulates differentiation of embryonic stem cells

A stem cell-derived gene (Sddr) negatively regulates differentiation of embryonic stem cells

Maternal control of early mouse development

FIGLA, a Basic Helix-Loop-Helix Transcription Factor, Balances Sexually Dimorphic Gene Expression in Postnatal Oocytes

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