Ovalbumin and cholera toxin delivery to buccal mucus for immunization using microneedles and comparison of immunological response to transmucosal delivery

Oh, Yujeong; Cha, Hye‐Ran; Hwang, Su Jin; Kim, Dae-Sung; Choi, Yeon Joo; Kim, Yun-Seo; Shin, Yura; Nguyen, Thuy Trang; Choi, Seong-O; Lee, Jae Myun; Park, Jung Hwan

doi:10.1007/s13346-021-00964-z

Cited by 24 publications

(22 citation statements)

References 40 publications

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“…The oral mucosa is an attractive site for vaccination, but a water rich environment can limit accurate dose delivery of vaccines. Ovalbumin dip coated on the tips of microneedle patch was found to deliver vaccine into the epithelium of the mice buccal mucosa in a short period of time compared to flat disk patch coated with ovalbumin substrate without microneedles [ 196 ]. The advent of nanohybrid materials as drug-delivery systems can hold the beneficial properties of their precursors and present additional advantages namely versatile methods for their production, improved mechanical and thermal stability, higher capacity of co-loading multiple drugs and diagnostic agents with diverse characteristics [ 193 ].…”

Section: Future Perspectives and Directionsmentioning

confidence: 99%

An Updated Overview of the Emerging Role of Patch and Film-Based Buccal Delivery Systems

et al. 2021

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Buccal mucosal membrane offers an attractive drug-delivery route to enhance both systemic and local therapy. This review discusses the benefits and drawbacks of buccal drug delivery, anatomical and physiological aspects of oral mucosa, and various in vitro techniques frequently used for examining buccal drug-delivery systems. The role of mucoadhesive polymers, penetration enhancers, and enzyme inhibitors to circumvent the formulation challenges particularly due to salivary renovation cycle, masticatory effect, and limited absorption area are summarized. Biocompatible mucoadhesive films and patches are favored dosage forms for buccal administration because of flexibility, comfort, lightness, acceptability, capacity to withstand mechanical stress, and customized size. Preparation methods, scale-up process and manufacturing of buccal films are briefed. Ongoing and completed clinical trials of buccal film formulations designed for systemic delivery are tabulated. Polymeric or lipid nanocarriers incorporated in buccal film to resolve potential formulation and drug-delivery issues are reviewed. Vaccine-enabled buccal films have the potential ability to produce both antibodies mediated and cell mediated immunity. Advent of novel 3D printing technologies with built-in flexibility would allow multiple drug combinations as well as compartmentalization to separate incompatible drugs. Exploring new functional excipients with potential capacity for permeation enhancement of particularly large-molecular-weight hydrophilic drugs and unstable proteins, oligonucleotides are the need of the hour for rapid advancement in the exciting field of buccal drug delivery.

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Section: Future Perspectives and Directionsmentioning

confidence: 99%

An Updated Overview of the Emerging Role of Patch and Film-Based Buccal Delivery Systems

et al. 2021

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“…Solid microneedles in an array were coated with ovalbumin, and the microneedle array was applied to murine buccal mucosa twice two weeks apart. A high serum IgG titer was subsequently observed, thus validating this approach [ 132 ].…”

Section: Buccal Absorptionmentioning

confidence: 91%

Roles of Lipids in the Permeability Barriers of Skin and Oral Mucosa

Wertz

2021

IJMS

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PubMed searches reveal much literature regarding lipids in barrier function of skin and less literature on lipids in barrier function of the oral mucosa. In terrestrial mammals, birds, and reptiles, the skin’s permeability barrier is provided by ceramides, fatty acids, and cholesterol in the outermost layers of the epidermis, the stratum corneum. This layer consists of about 10–20 layers of cornified cells embedded in a lipid matrix. It effectively prevents loss of water and electrolytes from the underlying tissue, and it limits the penetration of potentially harmful substances from the environment. In the oral cavity, the regions of the gingiva and hard palate are covered by keratinized epithelia that much resemble the epidermis. The oral stratum corneum contains a lipid mixture similar to that in the epidermal stratum corneum but in lower amounts and is accordingly more permeable. The superficial regions of the nonkeratinized oral epithelia also provide a permeability barrier. These epithelial regions do contain ceramides, cholesterol, and free fatty acids, which may underlie barrier function. The oral epithelial permeability barriers primarily protect the underlying tissue by preventing the penetration of potentially toxic substances, including microbial products. Transdermal drug delivery, buccal absorption, and lipid-related disease are discussed.

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“…The oral cavity shows great potential for antigen delivery. Various dosage forms have been developed that use the oral cavity as the site of administration, with tablets [71], mucoadhesive films [5,6,[71][72][73], gels [74], microneedles [75][76][77][78], and MucoJet™ [79] being reported (Table 2). The routes that comprise the oral cavity can be beneficial in many cases, increasing patient compliance because of its ease of administration and allowing a large-scale immunization.…”

Section: Antigen Delivery Systems and Commonly Used Mucosal Adjuvantsmentioning

confidence: 99%

“…Regarding delivery efficiency, it can be mentioned that it is a parameter that is closely related to the nature of the materials, specifically to their hydrophilicity. It has been seen in subsequent studies that for hydrophilic coatings, the delivery efficiency is much higher in both human and mouse skin models compared to coatings of a hydrophobic nature [76,86]. Another parameter to consider is the thickness of the coating, which has been related to a lower delivery efficiency [86].…”

Section: Microneedle Arraymentioning

confidence: 99%

Buccal and Sublingual Vaccines: A Review on Oral Mucosal Immunization and Delivery Systems

2021

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Currently, most vaccines available on the market are for parental use; however, this may not be the best option on several occasions. Mucosal routes of administration such as intranasal, sublingual, and buccal generate great interest due to the benefits they offer. These range from increasing patient compliance to inducing a more effective immune response than that achieved through conventional routes. Due to the activation of the common mucosal immune system, it is possible to generate an effective systemic and local immune response, which is not achieved through parenteral administration. Protection against pathogens that use mucosal entry routes is provided by an effective induction of mucosal immunity. Mucosal delivery systems are being developed, such as films and microneedles, which have proven to be effective, safe, and easy to administer. These systems have multiple advantages over commonly used injections, which are simple to manufacture, stable at room temperature, painless for the patient since they do not require puncture. Therefore, these delivery systems do not require to be administered by medical personnel; in fact, they could be self-administered.

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Ovalbumin and cholera toxin delivery to buccal mucus for immunization using microneedles and comparison of immunological response to transmucosal delivery

Cited by 24 publications

References 40 publications

An Updated Overview of the Emerging Role of Patch and Film-Based Buccal Delivery Systems

An Updated Overview of the Emerging Role of Patch and Film-Based Buccal Delivery Systems

Roles of Lipids in the Permeability Barriers of Skin and Oral Mucosa

Buccal and Sublingual Vaccines: A Review on Oral Mucosal Immunization and Delivery Systems

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