Outside Front Cover: Plasma Process. Polym. 3/2022

Self Cite

Plasma-treated water solutions (PTWS) allow the delivery of reactive oxygen and nitrogen species (RONS) to cells and tissues for different purposes. The mechanism of RONS formation has been clearly modelled in simple liquids like water, by assuming a plasma-driven process independent from the liquid. PTWS for biological experiments, however, are often produced from solutions of complex composition, where the formation mechanism of RONS is far from being understood. In this paper, we describe how water, phosphate-buffered saline solution and two cell culture media were plasma-treated in different conditions to demonstrate how the different composition of the liquids affects the formation of stable RONS (H 2 O 2 and NO 2 − ) in the resulting PTWS, especially when aromatic organic molecules are present.

Section: Methodsmentioning

confidence: 99%

“…N. 223-9955; Bio-Rad) with a volume of 1 ml and 10 mm optical length were used. Further information about the validation of each assay in the specific case of cell culture media and PTWS can be found in our reference [48].…”

Section: Electrical Characterization Of the Plasmamentioning

confidence: 99%

The active role of organic molecules in the formation of long‐lived reactive oxygen and nitrogen species in plasma‐treated water solutions

Veronico

Favia

Fracassi

et al. 2021

Self Cite

“…N. 1.14776.001, Merck Millipore) was used for qualitative and quantitative analysis of NO 2 − ions in PTWS: 13 mg of reagents powder of the kit were added to 1 ml of the liquid sample and left to react for 10 min before measuring the absorbance at 525 nm. An optimized protocol based on copper-neocuproine [47] was used to detect N. 223-9955, Bio-Rad) with a semi-micro volume of 1 ml and 10 mm optical length were used. Both assays were specifically validated in the case of PTWS as shown in a previous study.…”

Section: Characterization Of Liquid Samplesmentioning

confidence: 99%

“…Both assays were specifically validated in the case of PTWS as shown in a previous study. [47] Statistical analyses of data were computed using GraphPad Prism software version 6.1.…”

Section: Characterization Of Liquid Samplesmentioning

confidence: 99%

“…[8,29] In the last case, the proportion of the N 2 /O 2 mixture can be adjusted to modulate the relative amount between oxygen (ROS) and nitrogen species (RNS). [41] Depending on the treatment modalities, although, the water vapor from the liquid sample can combine with the feed, contributing to the composition of the discharge. [42][43][44] The presence of humidity in the discharge particularly impacts the generation of H 2 O 2 , which is one of the most bio-relevant species in PTWS and is also present already in cells.…”

mentioning

confidence: 99%

See 1 more Smart Citation

The effect of different cold atmospheric plasma sources and treatment modalities on the generation of reactive oxygen and nitrogen species in water

Armenise

Veronico

Cosmai

et al. 2022

Reactive oxygen and nitrogen species (RONS) can be generated in liquids by different cold plasma processes. These species can influence cell biology and pathological states, and trigger wound healing, disinfection, and cancer treatments. The optimization of plasma sources' configurations and experimental conditions is fundamental to dose-produced RONS in liquids. This research is a case study comparing RONS generation (H 2 O 2 and NO 2 − ) in water, after exposure to two different atmospheric pressure cold dielectric barrier discharge (DBD) plasma sources: a plasma jet-DBD and a planar-DBD operating in contact with the liquid and in remote, respectively. The data demonstrate that the reactor geometry and the treatment modality strongly impact the generation of the active species in water.

Chloramines of amino acids as precursors of bactericidal activity in cell culture media DMEM and RPMI treated by RF‐driven He/O₂ plasma jet

Jirásek

Tarabová

Lukeš

2023

Antibacterial properties and chemical changes of two culture media, Dulbecco's Modified Eagle Medium (DMEM) and Roswell Park Memorial Institute (RPMI), treated by RF‐driven He/O2 plasma jet, were studied using various chemical diagnostics and assessing Escherichia coli inactivation. The antibacterial effect increased with the plasma treatment time and was stronger in RPMI than in DMEM. Formation of organic mono‐ and dichloramines of amino acids in DMEM/RPMI by hypochlorite produced by the reaction of plasma‐generated O atoms with Cl− ions present in culture media were the major causes of the biocidal activity. This behavior was attributed to the action of tertiary chemical products formed by the decay of organic dichloramines. The thiobarbituric acid assay revealed malondialdehyde formation from glucose oxidation as the major component of the media.