Output grating couplers on planar optical waveguides as direct immunosensors

Lukosz, W.; Clerc, D.; Nellen, Philipp M.; Stamm, Ch.; Weiss, P.

doi:10.1016/0956-5663(91)80007-k

Cited by 76 publications

(27 citation statements)

References 4 publications

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“…OWLS is a highly sensitive method (precision $1 ng/cm 2 ) for measuring macromolecular adsorption kinetics at the solid-liquid interface [42][43][44][45]. Detection is based on excitation of guided modes via a polarized laser light beam directed upon a grating coupler at the surface of an optical waveguide.…”

Section: Owlsmentioning

confidence: 99%

Fibronectin terminated multilayer films: Protein adsorption and cell attachment studies

et al. 2007

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Electrostatically driven layer-by-layer (LbL) assembly is a simple and robust method for producing structurally tailored thin film biomaterials, of thickness ca. 10nm, containing biofunctional ligands. We investigate the LbL formation of multilayer films composed of polymers of biological origin (poly(L-lysine) (PLL) and dextran sulfate (DS)), the adsorption of fibronectin (Fn)--a matrix protein known to promote cell adhesion--onto these films, and the subsequent spreading behavior of human umbilical vein endothelial cells (HUVEC). We employ optical waveguide lightmode spectroscopy (OWLS) and quartz crystal microgravimetry with dissipation (QCMD) to characterize multilayer assembly in situ, and find adsorbed Fn mass on PLL-terminated films to exceed that on DS terminated films by 40%, correlating with the positive charge and lower degree of hydration of PLL terminated films. The extent and initial rate of Fn adsorption to both PLL and DS-terminated films exceed those onto the bare substrate, indicating the important role of electrostatic complexation between negatively charged protein and positively charged PLL at or near the film surface. We use phase-contrast optical microscopy to investigate the time-dependent morphological changes of HUVEC as a function of layer number, charge of terminal layer, and the presence of Fn. We observe HUVEC to attach, spread, and lose circularity on all surfaces. Positively charged PLL-terminated films exhibit a greater extent of cell spreading than do (negatively charged) DS-terminated films, and spreading is enhanced while circularity loss is suppressed by the presence of adsorbed Fn. The number of layers plays a significant role only for DS-terminated films with Fn, where spreading on a bilayer greatly exceeds that on a multilayer, and PLL-terminated films without Fn, where initial spreading is significantly higher on a monolayer. We observe initial cell spreading to be followed by retraction (i.e. decreased cell area and circularity with time) for films without Fn, and for DS-terminated films with Fn. Overall, the Fn-coated PLL monolayer and the Fn-coated PLL-terminated multilayer are the best performing films in promoting cell spreading. We conclude the presence of Fn to be an important factor (more so than film charge or layer number) in controlling the interaction between multilayer films and living cells, and thus to represent a promising strategy toward in vivo applications such as tissue engineering.

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Section: Owlsmentioning

confidence: 99%

Fibronectin terminated multilayer films: Protein adsorption and cell attachment studies

et al. 2007

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“…The biomaterial can be cladded on the surface of guide, where the clad acts as an interacting region to control the evanescent mode losses. For biological application, immobilized enzymes (Gouda et al, 2002;Gursel et al, 2003;Danisman et al, 2004;D'Souza and Godbole, 2002), proteins (Tsunoda et al, 1999;Cross et al, 2003) and other biomolecules such as antibodies (Adanyi et al, 2006;Lukosz et al, 1991) and peptides are now extensively used for immobilization.…”

Section: Introductionmentioning

confidence: 99%

Fabrication of sucrose biosensor based on single mode planar optical waveguide using co-immobilized plant invertase and GOD

Bagal

Vijayan

Aiyer

et al. 2007

Biosensors and Bioelectronics

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“…Biosensor systems such as grating couplers [22, 23] or interferometers [24-27] directly measure effective refractive index changes. The considerations above are only valid for nonporous waveguides, i.e.…”

Section: Introductionmentioning

confidence: 99%

Evanescent field Sensors Based on Tantalum Pentoxide Waveguides – A Review

Schmitt

Oehse

Sulz

et al. 2008

Sensors

115

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Evanescent field sensors based on waveguide surfaces play an important role where high sensitivity is required. Particularly tantalum pentoxide (Ta2O5) is a suitable material for thin-film waveguides due to its high refractive index and low attenuation. Many label-free biosensor systems such as grating couplers and interferometric sensors as well as fluorescence-based systems benefit from this waveguide material leading to extremely high sensitivity. Some biosensor systems based on Ta2O5 waveguides already took the step into commercialization. This report reviews the various detection systems in terms of limit of detection, the applications, and the suitable surface chemistry.

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Output grating couplers on planar optical waveguides as direct immunosensors

Cited by 76 publications

References 4 publications

Fibronectin terminated multilayer films: Protein adsorption and cell attachment studies

Fibronectin terminated multilayer films: Protein adsorption and cell attachment studies

Fabrication of sucrose biosensor based on single mode planar optical waveguide using co-immobilized plant invertase and GOD

Evanescent field Sensors Based on Tantalum Pentoxide Waveguides – A Review

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